8an6 - Revision history

OCA at 07:56, 1 May 2024

OCA — Wed, 01 May 2024 07:56:40 GMT

←Older revision		Revision as of 07:56, 1 May 2024
Line 10:		Line 10:
	== Function ==		== Function ==
	[https://www.uniprot.org/uniprot/A0A0N1ABZ3_9HYPH A0A0N1ABZ3_9HYPH]		[https://www.uniprot.org/uniprot/A0A0N1ABZ3_9HYPH A0A0N1ABZ3_9HYPH]
-	<div style="background-color:#fffaf0;">
-	== Publication Abstract from PubMed ==
-	The dimeric avidin family has been expanded in recent years to include many new members. All of them lack the intermonomeric Trp that plays a critical role in biotin-binding. Nevertheless, these new members of the avidins maintain the high affinity towards biotin. Additionally, all of the dimeric avidins share a very unique property: namely, the cylindrical oligomerization in the crystal structure. The newest member described here, agroavidin from the agrobacterium, Rhizobium sp. AAP43, shares their important structural features. However, the affinity of agroavidin towards biotin is lower than all other members of the avidin family, due to the presence of phenylalanine instead of a conserved tyrosine in the biotin-binding site. Mutating this phenylalanine into tyrosine regenerated the high affinity, which emphasizes the importance of this particular tyrosine residue. Another unique feature that distinguishes agroavidin from the other dimeric avidins is that it does not produce oligomers in its crystal structure. In order to understand the factors that promote oligomerization in dimeric avidins, we exchanged the C-terminal region of agroavidin with that of hoefavidin that produced octamers. This exchange resulted in a decamer rather than an octamer. This unusual outcome demonstrates the impact of the C-terminal region on the ability to produce oligomers. The decameric assembly of agroavidin expands the avidin-biotin toolbox even further and could well pave the path into new biotin-based technologies. Moreover, uncovering the factors that induce dimeric avidins into oligomeric assemblies may aid in better understanding the general molecular determinants that promote oligomerization.
-
-	Self-assembly of a dimeric avidin into unique higher-order oligomers.,Bana J, Warwar J, Bayer EA, Livnah O FEBS J. 2023 Feb 28. doi: 10.1111/febs.16764. PMID:36853192<ref>PMID:36853192</ref>
-
-	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	</div>
-	<div class="pdbe-citations 8an6" style="background-color:#fffaf0;"></div>
-	== References ==
-	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>

OCA at 11:10, 2 August 2023

OCA — Wed, 02 Aug 2023 11:10:21 GMT

←Older revision		Revision as of 11:10, 2 August 2023
Line 4:		Line 4:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[8an6]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Rhizobium_sp._AAP43 Rhizobium sp. AAP43]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8AN6 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8AN6 FirstGlance]. <br>		<table><tr><td colspan='2'>[[8an6]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Rhizobium_sp._AAP43 Rhizobium sp. AAP43]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8AN6 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8AN6 FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BTN:BIOTIN'>BTN</scene></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.9Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BTN:BIOTIN'>BTN</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8an6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8an6 OCA], [https://pdbe.org/8an6 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8an6 RCSB], [https://www.ebi.ac.uk/pdbsum/8an6 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8an6 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8an6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8an6 OCA], [https://pdbe.org/8an6 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8an6 RCSB], [https://www.ebi.ac.uk/pdbsum/8an6 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8an6 ProSAT]</span></td></tr>
	</table>		</table>

OCA at 20:29, 12 April 2023

OCA — Wed, 12 Apr 2023 20:29:45 GMT

←Older revision		Revision as of 20:29, 12 April 2023
Line 1:		Line 1:
-	'''Unreleased structure'''

-	The ~~entry~~ 8an6 is ~~ON HOLD until Paper~~ Publication	+	==The Crystal structure of wt apo agroavidin - biotin complex==
		+	<StructureSection load='8an6' size='340' side='right'caption='[[8an6]], [[Resolution\|resolution]] 1.90Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[8an6]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Rhizobium_sp._AAP43 Rhizobium sp. AAP43]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8AN6 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8AN6 FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BTN:BIOTIN'>BTN</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8an6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8an6 OCA], [https://pdbe.org/8an6 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8an6 RCSB], [https://www.ebi.ac.uk/pdbsum/8an6 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8an6 ProSAT]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/A0A0N1ABZ3_9HYPH A0A0N1ABZ3_9HYPH]
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The dimeric avidin family has been expanded in recent years to include many new members. All of them lack the intermonomeric Trp that plays a critical role in biotin-binding. Nevertheless, these new members of the avidins maintain the high affinity towards biotin. Additionally, all of the dimeric avidins share a very unique property: namely, the cylindrical oligomerization in the crystal structure. The newest member described here, agroavidin from the agrobacterium, Rhizobium sp. AAP43, shares their important structural features. However, the affinity of agroavidin towards biotin is lower than all other members of the avidin family, due to the presence of phenylalanine instead of a conserved tyrosine in the biotin-binding site. Mutating this phenylalanine into tyrosine regenerated the high affinity, which emphasizes the importance of this particular tyrosine residue. Another unique feature that distinguishes agroavidin from the other dimeric avidins is that it does not produce oligomers in its crystal structure. In order to understand the factors that promote oligomerization in dimeric avidins, we exchanged the C-terminal region of agroavidin with that of hoefavidin that produced octamers. This exchange resulted in a decamer rather than an octamer. This unusual outcome demonstrates the impact of the C-terminal region on the ability to produce oligomers. The decameric assembly of agroavidin expands the avidin-biotin toolbox even further and could well pave the path into new biotin-based technologies. Moreover, uncovering the factors that induce dimeric avidins into oligomeric assemblies may aid in better understanding the general molecular determinants that promote oligomerization.

-	~~Authors: Livnah, O~~., Bana, J.	+	Self-assembly of a dimeric avidin into unique higher-order oligomers.,Bana J, Warwar J, Bayer EA, Livnah O FEBS J. 2023 Feb 28. doi: 10.1111/febs.16764. PMID:36853192<ref>PMID:36853192</ref>

-	~~Description: The Crystal structure~~ of ~~wt apo agroavidin~~ -~~biotin complex~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased~~ Structures]]	+	</div>
-	[[Category: Bana, J]]	+	<div class="pdbe-citations 8an6" style="background-color:#fffaf0;"></div>
-	[[Category: Livnah, O]]	+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Rhizobium sp. AAP43]]
		+	[[Category: Bana J]]
		+	[[Category: Livnah O]]

OCA at 06:27, 21 September 2022

OCA — Wed, 21 Sep 2022 06:27:06 GMT

←Older revision		Revision as of 06:27, 21 September 2022
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 8an6 is ON HOLD	+	The entry 8an6 is ON HOLD until Paper Publication

	Authors: Livnah, O., Bana, J.		Authors: Livnah, O., Bana, J.

OCA: Protected "8an6" [edit=sysop:move=sysop]

OCA — Wed, 10 Aug 2022 03:58:57 GMT

Protected "8an6" [edit=sysop:move=sysop]

←Older revision

Revision as of 03:58, 10 August 2022

OCA: New page: '''Unreleased structure''' The entry 8an6 is ON HOLD Authors: Livnah, O., Bana, J. Description: The Crystal structure of wt apo agroavidin -biotin complex [[Category: Unreleased Struct...

OCA — Wed, 10 Aug 2022 03:58:56 GMT

New page: '''Unreleased structure''' The entry 8an6 is ON HOLD Authors: Livnah, O., Bana, J. Description: The Crystal structure of wt apo agroavidin -biotin complex [[Category: Unreleased Struct...

New page

'''Unreleased structure'''

The entry 8an6 is ON HOLD

Authors: Livnah, O., Bana, J.

Description: The Crystal structure of wt apo agroavidin -biotin complex
[[Category: Unreleased Structures]]
[[Category: Bana, J]]
[[Category: Livnah, O]]