OCA at 09:51, 15 March 2023

OCA — Wed, 15 Mar 2023 09:51:18 GMT

←Older revision		Revision as of 09:51, 15 March 2023
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-	'''Unreleased structure'''

-	~~The entry~~ 8b01 is ~~ON HOLD until Paper~~ Publication	+	==Cryo-EM structure of the Tripartite ATP-independent Periplasmic (TRAP) transporter SiaQM from Photobacterium profundum in a nanodisc==
		+	<StructureSection load='8b01' size='340' side='right'caption='[[8b01]], [[Resolution\|resolution]] 3.03Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[8b01]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Helicobacter_pylori Helicobacter pylori], [https://en.wikipedia.org/wiki/Photobacterium_profundum_SS9 Photobacterium profundum SS9] and [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8B01 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8B01 FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=D10:DECANE'>D10</scene>, <scene name='pdbligand=HEX:HEXANE'>HEX</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=OCT:N-OCTANE'>OCT</scene>, <scene name='pdbligand=PTY:PHOSPHATIDYLETHANOLAMINE'>PTY</scene>, <scene name='pdbligand=TRD:TRIDECANE'>TRD</scene>, <scene name='pdbligand=TWT:DOCOSANE'>TWT</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8b01 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8b01 OCA], [https://pdbe.org/8b01 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8b01 RCSB], [https://www.ebi.ac.uk/pdbsum/8b01 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8b01 ProSAT]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/Q6LPW0_PHOPR Q6LPW0_PHOPR] Part of the tripartite ATP-independent periplasmic (TRAP) transport system.[RuleBase:RU369079]
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	In bacteria and archaea, tripartite ATP-independent periplasmic (TRAP) transporters uptake essential nutrients. TRAP transporters receive their substrates via a secreted soluble substrate-binding protein. How a sodium ion-driven secondary active transporter is strictly coupled to a substrate-binding protein is poorly understood. Here we report the cryo-EM structure of the sialic acid TRAP transporter SiaQM from Photobacterium profundum at 2.97 A resolution. SiaM comprises a "transport" domain and a "scaffold" domain, with the transport domain consisting of helical hairpins as seen in the sodium ion-coupled elevator transporter VcINDY. The SiaQ protein forms intimate contacts with SiaM to extend the size of the scaffold domain, suggesting that TRAP transporters may operate as monomers, rather than the typically observed oligomers for elevator-type transporters. We identify the Na(+) and sialic acid binding sites in SiaM and demonstrate a strict dependence on the substrate-binding protein SiaP for uptake. We report the SiaP crystal structure that, together with docking studies, suggest the molecular basis for how sialic acid is delivered to the SiaQM transporter complex. We thus propose a model for substrate transport by TRAP proteins, which we describe herein as an 'elevator-with-an-operator' mechanism.

-	~~Authors~~:	+	Structure and mechanism of a tripartite ATP-independent periplasmic TRAP transporter.,Davies JS, Currie MJ, North RA, Scalise M, Wright JD, Copping JM, Remus DM, Gulati A, Morado DR, Jamieson SA, Newton-Vesty MC, Abeysekera GS, Ramaswamy S, Friemann R, Wakatsuki S, Allison JR, Indiveri C, Drew D, Mace PD, Dobson RCJ Nat Commun. 2023 Feb 27;14(1):1120. doi: 10.1038/s41467-023-36590-1. PMID:36849793<ref>PMID:36849793</ref>

-	~~Description~~:	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased~~ Structures]]	+	</div>
		+	<div class="pdbe-citations 8b01" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Helicobacter pylori]]
		+	[[Category: Large Structures]]
		+	[[Category: Photobacterium profundum SS9]]
		+	[[Category: Synthetic construct]]
		+	[[Category: Davies JS]]
		+	[[Category: Dobson RCJ]]
		+	[[Category: North RA]]

OCA at 16:43, 19 October 2022

OCA — Wed, 19 Oct 2022 16:43:27 GMT

←Older revision		Revision as of 16:43, 19 October 2022
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 8b01 is ON HOLD	+	The entry 8b01 is ON HOLD until Paper Publication

	Authors:		Authors:

OCA: Protected "8b01" [edit=sysop:move=sysop]

OCA — Wed, 14 Sep 2022 05:41:06 GMT

Protected "8b01" [edit=sysop:move=sysop]

←Older revision

Revision as of 05:41, 14 September 2022

OCA: New page: '''Unreleased structure''' The entry 8b01 is ON HOLD Authors: Description: Category: Unreleased Structures

OCA — Wed, 14 Sep 2022 05:41:05 GMT

New page: '''Unreleased structure''' The entry 8b01 is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 8b01 is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]

8b01 - Revision history

OCA at 09:51, 15 March 2023

OCA at 16:43, 19 October 2022

OCA: Protected "8b01" [edit=sysop:move=sysop]

OCA: New page: '''Unreleased structure''' The entry 8b01 is ON HOLD Authors: Description: Category: Unreleased Structures