8b48 - Revision history

OCA at 08:16, 7 February 2024

OCA — Wed, 07 Feb 2024 08:16:13 GMT

←Older revision		Revision as of 08:16, 7 February 2024
Line 4:		Line 4:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[8b48]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Lentithecium_fluviatile Lentithecium fluviatile]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8B48 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8B48 FirstGlance]. <br>		<table><tr><td colspan='2'>[[8b48]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Lentithecium_fluviatile Lentithecium fluviatile]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8B48 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8B48 FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BMA:BETA-D-MANNOSE'>BMA</scene>, <scene name='pdbligand=FMT:FORMIC+ACID'>FMT</scene>, <scene name='pdbligand=MAN:ALPHA-D-MANNOSE'>MAN</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.65Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BMA:BETA-D-MANNOSE'>BMA</scene>, <scene name='pdbligand=FMT:FORMIC+ACID'>FMT</scene>, <scene name='pdbligand=MAN:ALPHA-D-MANNOSE'>MAN</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8b48 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8b48 OCA], [https://pdbe.org/8b48 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8b48 RCSB], [https://www.ebi.ac.uk/pdbsum/8b48 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8b48 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8b48 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8b48 OCA], [https://pdbe.org/8b48 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8b48 RCSB], [https://www.ebi.ac.uk/pdbsum/8b48 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8b48 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
	[https://www.uniprot.org/uniprot/A0A6G1IIU9_9PLEO A0A6G1IIU9_9PLEO]		[https://www.uniprot.org/uniprot/A0A6G1IIU9_9PLEO A0A6G1IIU9_9PLEO]
-	<div style="background-color:#fffaf0;">
-	== Publication Abstract from PubMed ==
-	In plant cell walls, covalent bonds between polysaccharides and lignin increase recalcitrance to degradation. Ester bonds are known to exist between glucuronic acid moieties on glucuronoxylan and lignin, and these can be cleaved by glucuronoyl esterases (GEs) from carbohydrate esterase family 15 (CE15). GEs are found in both bacteria and fungi, and some microorganisms also encode multiple GEs, although the reason for this is still not fully clear. The fungus Lentithecium fluviatile encodes three CE15 enzymes, of which two have previously been heterologously produced, although neither was active on the tested model substrate. Here, one of these, LfCE15C, has been investigated in detail using a range of model and natural substrates and its structure has been solved using X-ray crystallography. No activity could be verified on any tested substrate, but biophysical assays indicate an ability to bind to complex carbohydrate ligands. The structure further suggests that this enzyme, which possesses an intact catalytic triad, might be able to bind and act on more extensively decorated xylan chains than has been reported for other CE15 members. It is speculated that rare glucuronoxylans decorated at the glucuronic acid moiety may be the true targets of LfCE15C and other CE15 family members with similar sequence characteristics.
-
-	Structural and functional investigation of a fungal member of carbohydrate esterase family 15 with potential specificity for rare xylans.,Mazurkewich S, Scholzen KC, Brusch RH, Poulsen JCN, Theibich Y, Huttner S, Olsson L, Larsbrink J, Lo Leggio L Acta Crystallogr D Struct Biol. 2023 Jun 1;79(Pt 6):545-555. doi: , 10.1107/S205979832300325X. Epub 2023 May 25. PMID:37227091<ref>PMID:37227091</ref>
-
-	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	</div>
-	<div class="pdbe-citations 8b48" style="background-color:#fffaf0;"></div>
-	== References ==
-	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>

OCA at 05:37, 7 June 2023

OCA — Wed, 07 Jun 2023 05:37:11 GMT

←Older revision		Revision as of 05:37, 7 June 2023
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 8b48 is ~~ON HOLD until Paper~~ Publication	+	==Structure of Lentithecium fluviatile carbohydrate esterase from the CE15 family (LfCE15C)==
		+	<StructureSection load='8b48' size='340' side='right'caption='[[8b48]], [[Resolution\|resolution]] 2.65Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[8b48]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Lentithecium_fluviatile Lentithecium fluviatile]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8B48 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8B48 FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BMA:BETA-D-MANNOSE'>BMA</scene>, <scene name='pdbligand=FMT:FORMIC+ACID'>FMT</scene>, <scene name='pdbligand=MAN:ALPHA-D-MANNOSE'>MAN</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8b48 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8b48 OCA], [https://pdbe.org/8b48 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8b48 RCSB], [https://www.ebi.ac.uk/pdbsum/8b48 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8b48 ProSAT]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/A0A6G1IIU9_9PLEO A0A6G1IIU9_9PLEO]
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	In plant cell walls, covalent bonds between polysaccharides and lignin increase recalcitrance to degradation. Ester bonds are known to exist between glucuronic acid moieties on glucuronoxylan and lignin, and these can be cleaved by glucuronoyl esterases (GEs) from carbohydrate esterase family 15 (CE15). GEs are found in both bacteria and fungi, and some microorganisms also encode multiple GEs, although the reason for this is still not fully clear. The fungus Lentithecium fluviatile encodes three CE15 enzymes, of which two have previously been heterologously produced, although neither was active on the tested model substrate. Here, one of these, LfCE15C, has been investigated in detail using a range of model and natural substrates and its structure has been solved using X-ray crystallography. No activity could be verified on any tested substrate, but biophysical assays indicate an ability to bind to complex carbohydrate ligands. The structure further suggests that this enzyme, which possesses an intact catalytic triad, might be able to bind and act on more extensively decorated xylan chains than has been reported for other CE15 members. It is speculated that rare glucuronoxylans decorated at the glucuronic acid moiety may be the true targets of LfCE15C and other CE15 family members with similar sequence characteristics.

-	~~Authors~~:	+	Structural and functional investigation of a fungal member of carbohydrate esterase family 15 with potential specificity for rare xylans.,Mazurkewich S, Scholzen KC, Brusch RH, Poulsen JCN, Theibich Y, Huttner S, Olsson L, Larsbrink J, Lo Leggio L Acta Crystallogr D Struct Biol. 2023 Jun 1;79(Pt 6):545-555. doi: , 10.1107/S205979832300325X. Epub 2023 May 25. PMID:37227091<ref>PMID:37227091</ref>

-	~~Description~~:	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased~~ Structures]]	+	</div>
		+	<div class="pdbe-citations 8b48" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Lentithecium fluviatile]]
		+	[[Category: Larsbrink J]]
		+	[[Category: Lo Leggio L]]
		+	[[Category: Mazurkewich S]]
		+	[[Category: Poulsen JCN]]
		+	[[Category: Scholzen K]]

OCA at 05:06, 3 November 2022

OCA — Thu, 03 Nov 2022 05:06:47 GMT

←Older revision		Revision as of 05:06, 3 November 2022
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 8b48 is ON HOLD	+	The entry 8b48 is ON HOLD until Paper Publication

	Authors:		Authors:

OCA: Protected "8b48" [edit=sysop:move=sysop]

OCA — Wed, 28 Sep 2022 04:36:30 GMT

Protected "8b48" [edit=sysop:move=sysop]

←Older revision

Revision as of 04:36, 28 September 2022

OCA: New page: '''Unreleased structure''' The entry 8b48 is ON HOLD Authors: Description: Category: Unreleased Structures

OCA — Wed, 28 Sep 2022 04:36:29 GMT

New page: '''Unreleased structure''' The entry 8b48 is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 8b48 is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]