OCA at 05:52, 23 August 2023

OCA — Wed, 23 Aug 2023 05:52:59 GMT

←Older revision		Revision as of 05:52, 23 August 2023
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 8bb1 is ~~ON HOLD until Paper~~ Publication	+	==T3 SAM lyase in complex with S-adenosylmethionine synthase==
		+	<StructureSection load='8bb1' size='340' side='right'caption='[[8bb1]], [[Resolution\|resolution]] 2.80Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[8bb1]] is a 8 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] and [https://en.wikipedia.org/wiki/Teetrevirus Teetrevirus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8BB1 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8BB1 FirstGlance]. <br>
		+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution\|Resolution]] 2.8Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8bb1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8bb1 OCA], [https://pdbe.org/8bb1 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8bb1 RCSB], [https://www.ebi.ac.uk/pdbsum/8bb1 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8bb1 ProSAT]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/METK_ECOLI METK_ECOLI] Catalyzes the formation of S-adenosylmethionine from methionine and ATP. The overall synthetic reaction is composed of two sequential steps, AdoMet formation and the subsequent tripolyphosphate hydrolysis which occurs prior to release of AdoMet from the enzyme. Is essential for growth.[HAMAP-Rule:MF_00086]
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Bacteriophage T3 encodes a SAMase that, through cleavage of S-adenosyl methionine (SAM), circumvents the SAM-dependent type I restriction-modification (R-M) defense. We show that SAMase also allows T3 to evade the BREX defense. Although SAM depletion weakly affects BREX methylation, it completely inhibits the defensive function of BREX, suggesting that SAM could be a co-factor for BREX-mediated exclusion of phage DNA, similar to its anti-defense role in type I R-M. The anti-BREX activity of T3 SAMase is mediated not just by enzymatic degradation of SAM but also by direct inhibition of MetK, the host SAM synthase. We present a 2.8 A cryoelectron microscopy (cryo-EM) structure of the eight-subunit T3 SAMase-MetK complex. Structure-guided mutagenesis reveals that this interaction stabilizes T3 SAMase in vivo, further stimulating its anti-BREX activity. This work provides insights in the versatility of bacteriophage counterdefense mechanisms and highlights the role of SAM as a co-factor of diverse bacterial immunity systems.

-	~~Authors: Triguis~~, S., ~~Selmer~~, M.	+	Phage T3 overcomes the BREX defense through SAM cleavage and inhibition of SAM synthesis by SAM lyase.,Andriianov A, Triguis S, Drobiazko A, Sierro N, Ivanov NV, Selmer M, Severinov K, Isaev A Cell Rep. 2023 Aug 13;42(8):112972. doi: 10.1016/j.celrep.2023.112972. PMID:37578860<ref>PMID:37578860</ref>

-	~~Description: T3 SAM lyase in complex with~~ S-~~adenosylmethionine synthase~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased~~ Structures]]	+	</div>
-	[[Category: Selmer, M]]	+	<div class="pdbe-citations 8bb1" style="background-color:#fffaf0;"></div>
-	[[Category: Triguis, S]]	+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Escherichia coli]]
		+	[[Category: Large Structures]]
		+	[[Category: Teetrevirus]]
		+	[[Category: Selmer M]]
		+	[[Category: Triguis S]]

OCA at 04:48, 9 November 2022

OCA — Wed, 09 Nov 2022 04:48:25 GMT

←Older revision		Revision as of 04:48, 9 November 2022
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 8bb1 is ON HOLD	+	The entry 8bb1 is ON HOLD until Paper Publication

	Authors: Triguis, S., Selmer, M.		Authors: Triguis, S., Selmer, M.

OCA: Protected "8bb1" [edit=sysop:move=sysop]

OCA — Wed, 19 Oct 2022 17:03:14 GMT

Protected "8bb1" [edit=sysop:move=sysop]

←Older revision

Revision as of 17:03, 19 October 2022

OCA: New page: '''Unreleased structure''' The entry 8bb1 is ON HOLD Authors: Triguis, S., Selmer, M. Description: T3 SAM lyase in complex with S-adenosylmethionine synthase [[Category: Unreleased Str...

OCA — Wed, 19 Oct 2022 17:03:12 GMT

New page: '''Unreleased structure''' The entry 8bb1 is ON HOLD Authors: Triguis, S., Selmer, M. Description: T3 SAM lyase in complex with S-adenosylmethionine synthase [[Category: Unreleased Str...

New page

'''Unreleased structure'''

The entry 8bb1 is ON HOLD

Authors: Triguis, S., Selmer, M.

Description: T3 SAM lyase in complex with S-adenosylmethionine synthase
[[Category: Unreleased Structures]]
[[Category: Selmer, M]]
[[Category: Triguis, S]]

8bb1 - Revision history

OCA at 05:52, 23 August 2023

OCA at 04:48, 9 November 2022

OCA: Protected "8bb1" [edit=sysop:move=sysop]

OCA: New page: '''Unreleased structure''' The entry 8bb1 is ON HOLD Authors: Triguis, S., Selmer, M. Description: T3 SAM lyase in complex with S-adenosylmethionine synthase [[Category: Unreleased Str...