8bet - Revision history

OCA at 13:39, 1 February 2024

OCA — Thu, 01 Feb 2024 13:39:35 GMT

←Older revision		Revision as of 13:39, 1 February 2024
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	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[8bet]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Rhodotorula_dairenensis Rhodotorula dairenensis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8BET OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8BET FirstGlance]. <br>		<table><tr><td colspan='2'>[[8bet]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Rhodotorula_dairenensis Rhodotorula dairenensis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8BET OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8BET FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BMA:BETA-D-MANNOSE'>BMA</scene>, <scene name='pdbligand=FRU:FRUCTOSE'>FRU</scene>, <scene name='pdbligand=GLC:ALPHA-D-GLUCOSE'>GLC</scene>, <scene name='pdbligand=MAN:ALPHA-D-MANNOSE'>MAN</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=PRD_900003:sucrose'>PRD_900003</scene></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.38Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BMA:BETA-D-MANNOSE'>BMA</scene>, <scene name='pdbligand=FRU:FRUCTOSE'>FRU</scene>, <scene name='pdbligand=GLC:ALPHA-D-GLUCOSE'>GLC</scene>, <scene name='pdbligand=MAN:ALPHA-D-MANNOSE'>MAN</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=PRD_900003:sucrose'>PRD_900003</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8bet FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8bet OCA], [https://pdbe.org/8bet PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8bet RCSB], [https://www.ebi.ac.uk/pdbsum/8bet PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8bet ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8bet FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8bet OCA], [https://pdbe.org/8bet PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8bet RCSB], [https://www.ebi.ac.uk/pdbsum/8bet PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8bet ProSAT]</span></td></tr>
	</table>		</table>

OCA at 09:08, 28 December 2022

OCA — Wed, 28 Dec 2022 09:08:04 GMT

←Older revision		Revision as of 09:08, 28 December 2022
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 8bet is ~~ON HOLD until Paper~~ Publication	+	==Structure of D188A-fructofuranosidase from Rhodotorula dairenesis in complex with sucrose==
		+	<StructureSection load='8bet' size='340' side='right'caption='[[8bet]], [[Resolution\|resolution]] 2.38Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[8bet]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Rhodotorula_dairenensis Rhodotorula dairenensis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8BET OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8BET FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BMA:BETA-D-MANNOSE'>BMA</scene>, <scene name='pdbligand=FRU:FRUCTOSE'>FRU</scene>, <scene name='pdbligand=GLC:ALPHA-D-GLUCOSE'>GLC</scene>, <scene name='pdbligand=MAN:ALPHA-D-MANNOSE'>MAN</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=PRD_900003:sucrose'>PRD_900003</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8bet FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8bet OCA], [https://pdbe.org/8bet PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8bet RCSB], [https://www.ebi.ac.uk/pdbsum/8bet PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8bet ProSAT]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/A0A856TAI5_9BASI A0A856TAI5_9BASI]
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Rhodotorula dairenensis beta-fructofuranosidase is a highly glycosylated enzyme with broad substrate specificity that catalyzes the synthesis of 6-kestose and a mixture of the three series of fructooligosaccharides (FOS), fructosylating a variety of carbohydrates and other molecules as alditols. We report here its three-dimensional structure, showing the expected bimodular arrangement and also a unique long elongation at its N-terminus containing extensive O-glycosylation sites that form a peculiar arrangement with a protruding loop within the dimer. This region is not required for activity but could provide a molecular tool to target the dimeric protein to its receptor cellular compartment in the yeast. A truncated inactivated form was used to obtain complexes with fructose, sucrose and raffinose, and a Bis-Tris molecule was trapped, mimicking a putative acceptor substrate. The crystal structure of the complexes reveals the major traits of the active site, with Asn387 controlling the substrate binding mode. Relevant residues were selected for mutagenesis, the variants being biochemically characterized through their hydrolytic and transfructosylating activity. All changes decrease the hydrolytic efficiency against sucrose, proving their key role in the activity. Moreover, some of the generated variants exhibit redesigned transfructosylating specificity, which may be used for biotechnological purposes to produce novel fructosyl-derivatives.

-	~~Authors~~:	+	Insights into the Structure of the Highly Glycosylated Ffase from Rhodotorula dairenensis Enhance Its Biotechnological Potential.,Jimenez-Ortega E, Narmontaite E, Gonzalez-Perez B, Plou FJ, Fernandez-Lobato M, Sanz-Aparicio J Int J Mol Sci. 2022 Nov 29;23(23):14981. doi: 10.3390/ijms232314981. PMID:36499311<ref>PMID:36499311</ref>

-	~~Description~~:	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased~~ Structures]]	+	</div>
		+	<div class="pdbe-citations 8bet" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Rhodotorula dairenensis]]
		+	[[Category: Jimenez-Ortega E]]
		+	[[Category: Sanz-Aparicio J]]

OCA at 07:49, 24 November 2022

OCA — Thu, 24 Nov 2022 07:49:33 GMT

←Older revision		Revision as of 07:49, 24 November 2022
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 8bet is ON HOLD	+	The entry 8bet is ON HOLD until Paper Publication

	Authors:		Authors:

OCA: Protected "8bet" [edit=sysop:move=sysop]

OCA — Thu, 03 Nov 2022 05:10:16 GMT

Protected "8bet" [edit=sysop:move=sysop]

←Older revision

Revision as of 05:10, 3 November 2022

OCA: New page: '''Unreleased structure''' The entry 8bet is ON HOLD Authors: Description: Category: Unreleased Structures

OCA — Thu, 03 Nov 2022 05:10:15 GMT

New page: '''Unreleased structure''' The entry 8bet is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 8bet is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]