OCA at 08:20, 16 August 2023

OCA — Wed, 16 Aug 2023 08:20:27 GMT

←Older revision		Revision as of 08:20, 16 August 2023
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-	'''Unreleased structure'''

-	~~The entry~~ 8blw is ~~ON HOLD until Paper~~ Publication	+	==Vitamin B12 transporter BtuB1 with lipoprotein BtuG1 from B. theta==
		+	<StructureSection load='8blw' size='340' side='right'caption='[[8blw]], [[Resolution\|resolution]] 3.22Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[8blw]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Bacteroides_thetaiotaomicron_VPI-5482 Bacteroides thetaiotaomicron VPI-5482]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8BLW OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8BLW FirstGlance]. <br>
		+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution\|Resolution]] 3.22Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GAL:BETA-D-GALACTOSE'>GAL</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8blw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8blw OCA], [https://pdbe.org/8blw PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8blw RCSB], [https://www.ebi.ac.uk/pdbsum/8blw PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8blw ProSAT]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/Q8A7N5_BACTN Q8A7N5_BACTN]
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Vitamin B(12) (cobalamin) is required for most human gut microbes, many of which are dependent on scavenging to obtain this vitamin. Since bacterial densities in the gut are extremely high, competition for this keystone micronutrient is severe. Contrasting with Enterobacteria, members of the dominant genus Bacteroides often encode several BtuB vitamin B(12) outer membrane transporters together with a conserved array of surface-exposed B(12)-binding lipoproteins. Here we show that the BtuB transporters from Bacteroides thetaiotaomicron form stable, pedal bin-like complexes with surface-exposed BtuG lipoprotein lids, which bind B(12) with high affinities. Closing of the BtuG lid following B(12) capture causes destabilisation of the bound B(12) by a conserved BtuB extracellular loop, causing translocation of the vitamin to BtuB and subsequent transport. We propose that TonB-dependent, lipoprotein-assisted small molecule uptake is a general feature of Bacteroides spp. that is important for the success of this genus in colonising the human gut.

-	~~Authors~~:	+	BtuB TonB-dependent transporters and BtuG surface lipoproteins form stable complexes for vitamin B(12) uptake in gut Bacteroides.,Abellon-Ruiz J, Jana K, Silale A, Frey AM, Basle A, Trost M, Kleinekathofer U, van den Berg B Nat Commun. 2023 Aug 5;14(1):4714. doi: 10.1038/s41467-023-40427-2. PMID:37543597<ref>PMID:37543597</ref>

-	~~Description~~:	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased~~ Structures]]	+	</div>
		+	<div class="pdbe-citations 8blw" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Bacteroides thetaiotaomicron VPI-5482]]
		+	[[Category: Large Structures]]
		+	[[Category: Abellon-Ruiz J]]
		+	[[Category: Silale A]]
		+	[[Category: Van den Berg B]]

OCA: Protected "8blw" [edit=sysop:move=sysop]

OCA — Wed, 16 Nov 2022 18:00:55 GMT

Protected "8blw" [edit=sysop:move=sysop]

←Older revision

Revision as of 18:00, 16 November 2022

OCA: New page: '''Unreleased structure''' The entry 8blw is ON HOLD until Paper Publication Authors: Description: Category: Unreleased Structures

OCA — Wed, 16 Nov 2022 18:00:52 GMT

New page: '''Unreleased structure''' The entry 8blw is ON HOLD until Paper Publication Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 8blw is ON HOLD until Paper Publication

Authors:

Description:
[[Category: Unreleased Structures]]

8blw - Revision history

OCA at 08:20, 16 August 2023

OCA: Protected "8blw" [edit=sysop:move=sysop]

OCA: New page: '''Unreleased structure''' The entry 8blw is ON HOLD until Paper Publication Authors: Description: Category: Unreleased Structures