OCA at 09:09, 28 December 2022

OCA — Wed, 28 Dec 2022 09:09:29 GMT

←Older revision		Revision as of 09:09, 28 December 2022
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-	'''Unreleased structure'''

-	~~The entry~~ 8bqe is ~~ON HOLD~~	+	==In situ structure of the Caulobacter crescentus S-layer==
		+	<StructureSection load='8bqe' size='340' side='right'caption='[[8bqe]], [[Resolution\|resolution]] 3.50Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[8bqe]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Caulobacter_vibrioides_NA1000 Caulobacter vibrioides NA1000]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8BQE OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8BQE FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BMA:BETA-D-MANNOSE'>BMA</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=MRH:4-acetamido-4,6-dideoxy-alpha-D-mannopyranose'>MRH</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8bqe FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8bqe OCA], [https://pdbe.org/8bqe PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8bqe RCSB], [https://www.ebi.ac.uk/pdbsum/8bqe PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8bqe ProSAT]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/A0A0H3C8J1_CAUVN A0A0H3C8J1_CAUVN]
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	We present a new approach for macromolecular structure determination from multiple particles in electron cryo-tomography (cryo-ET) data sets. Whereas existing subtomogram averaging approaches are based on 3D data models, we propose to optimise a regularised likelihood target that approximates a function of the 2D experimental images. In addition, analogous to Bayesian polishing and contrast transfer function (CTF) refinement in single-particle analysis, we describe approaches that exploit the increased signal-to-noise ratio in the averaged structure to optimise tilt series alignments, beam-induced motions of the particles throughout the tilt series acquisition, defoci of the individual particles, as well as higher-order optical aberrations of the microscope. Implementation of our approaches in the open-source software package RELION aims to facilitate their general use, in particular for those researchers who are already familiar with its single-particle analysis tools. We illustrate for three applications that our approaches allow structure determination from cryo-ET data to resolutions sufficient for de novo atomic modelling.

-	~~Authors: von Kuegelgen,~~ A., Bharat, T.	+	A Bayesian approach to single-particle electron cryo-tomography in RELION-4.0.,Zivanov J, Oton J, Ke Z, von Kugelgen A, Pyle E, Qu K, Morado D, Castano-Diez D, Zanetti G, Bharat TAM, Briggs JAG, Scheres SHW Elife. 2022 Dec 5;11:e83724. doi: 10.7554/eLife.83724. PMID:36468689<ref>PMID:36468689</ref>

-	~~Description: In situ structure~~ of the ~~Caulobacter crescentus~~ S-~~layer~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased~~ Structures]]	+	</div>
-	[[Category: Bharat, T]]	+	<div class="pdbe-citations 8bqe" style="background-color:#fffaf0;"></div>
-	[[Category: Von Kuegelgen, A]]	+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Caulobacter vibrioides NA1000]]
		+	[[Category: Large Structures]]
		+	[[Category: Bharat T]]
		+	[[Category: Von Kuegelgen A]]

OCA: Protected "8bqe" [edit=sysop:move=sysop]

OCA — Wed, 30 Nov 2022 08:23:58 GMT

Protected "8bqe" [edit=sysop:move=sysop]

←Older revision

Revision as of 08:23, 30 November 2022

OCA: New page: '''Unreleased structure''' The entry 8bqe is ON HOLD Authors: von Kuegelgen, A., Bharat, T. Description: In situ structure of the Caulobacter crescentus S-layer [[Category: Unreleased ...

OCA — Wed, 30 Nov 2022 08:23:55 GMT

New page: '''Unreleased structure''' The entry 8bqe is ON HOLD Authors: von Kuegelgen, A., Bharat, T. Description: In situ structure of the Caulobacter crescentus S-layer [[Category: Unreleased ...

New page

'''Unreleased structure'''

The entry 8bqe is ON HOLD

Authors: von Kuegelgen, A., Bharat, T.

Description: In situ structure of the Caulobacter crescentus S-layer
[[Category: Unreleased Structures]]
[[Category: Bharat, T]]
[[Category: Von Kuegelgen, A]]

8bqe - Revision history

OCA at 09:09, 28 December 2022

OCA: Protected "8bqe" [edit=sysop:move=sysop]

OCA: New page: '''Unreleased structure''' The entry 8bqe is ON HOLD Authors: von Kuegelgen, A., Bharat, T. Description: In situ structure of the Caulobacter crescentus S-layer [[Category: Unreleased ...