OCA at 06:02, 2 March 2023

OCA — Thu, 02 Mar 2023 06:02:52 GMT

←Older revision		Revision as of 06:02, 2 March 2023
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 8brp is ~~ON HOLD until Paper~~ Publication	+	==Peptide Arginase OspR from the cyanobacterium Kamptonema sp.==
		+	<StructureSection load='8brp' size='340' side='right'caption='[[8brp]], [[Resolution\|resolution]] 2.60Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[8brp]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Kamptonema Kamptonema]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8BRP OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8BRP FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene>, <scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8brp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8brp OCA], [https://pdbe.org/8brp PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8brp RCSB], [https://www.ebi.ac.uk/pdbsum/8brp PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8brp ProSAT]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/D8G6F7_9CYAN D8G6F7_9CYAN]
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Landornamide A is a ribosomally synthesized and post-translationally modified peptide (RiPP) natural product with antiviral activity. Its biosynthetic gene cluster encodes horizontal line among other maturases horizontal line the peptide arginase OspR, which converts arginine to ornithine units in an unusual post-translational modification. Peptide arginases are a recently discovered RiPP maturase family with few characterized representatives. They show little sequence similarity to conventional arginases, a well-characterized enzyme family catalyzing the hydrolysis of free arginine to ornithine and urea. Peptide arginases are highly promiscuous and accept a variety of substrate sequences. The molecular basis for binding the large peptide substrate and for the high promiscuity of peptide arginases remains unclear. Here, we report the first crystal structure of a peptide arginase at a resolution of 2.6 A. The three-dimensional structure reveals common features and differences between conventional arginases and the peptide arginase: the binuclear metal cluster and the active-site environment strongly resemble each other, while the quaternary structures diverge. Kinetic analyses of OspR with various substrates provide new insights into the order of biosynthetic reactions during the post-translational maturation of landornamide A. These results provide the basis for pathway engineering to generate derivatives of landornamide A and for the general application of peptide arginases as biosynthetic tools for peptide engineering.

-	~~Authors: Mordhorst~~, S., Badmann, T., ~~Boesch, N.M.~~, Morinaka, ~~B.I., Rach,~~ H., Piel, J., Groll, M~~., Vagstadt~~, A.L.	+	Structural and Biochemical Insights into Post-Translational Arginine-to-Ornithine Peptide Modifications by an Atypical Arginase.,Mordhorst S, Badmann T, Bosch NM, Morinaka BI, Rauch H, Piel J, Groll M, Vagstad AL ACS Chem Biol. 2023 Feb 15. doi: 10.1021/acschembio.2c00879. PMID:36791048<ref>PMID:36791048</ref>

-	~~Description: Peptide Arginase OspR from~~ the ~~cyanobacterium Kamptonema sp~~.	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased~~ Structures]]	+	</div>
-	[[Category: Badmann, T]]	+	<div class="pdbe-citations 8brp" style="background-color:#fffaf0;"></div>
-	[[Category: ~~Rach, H~~]]	+	== References ==
-	[[Category: ~~Morinaka, B.I~~]]	+	<references/>
-	[[Category: ~~Vagstadt, A.L~~]]	+	__TOC__
-	[[Category: ~~Piel, J~~]]	+	</StructureSection>
-	[[Category: ~~Groll, M~~]]	+	[[Category: Kamptonema]]
-	[[Category: ~~Mordhorst, S~~]]	+	[[Category: Large Structures]]
-	[[Category: ~~Boesch, N.M~~]]	+	[[Category: Badmann T]]
		+	[[Category: Boesch NM]]
		+	[[Category: Groll M]]
		+	[[Category: Mordhorst S]]
		+	[[Category: Morinaka BI]]
		+	[[Category: Piel J]]
		+	[[Category: Rach H]]
		+	[[Category: Vagstadt AL]]

OCA at 05:28, 28 December 2022

OCA — Wed, 28 Dec 2022 05:28:41 GMT

←Older revision		Revision as of 05:28, 28 December 2022
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 8brp is ON HOLD	+	The entry 8brp is ON HOLD until Paper Publication

	Authors: Mordhorst, S., Badmann, T., Boesch, N.M., Morinaka, B.I., Rach, H., Piel, J., Groll, M., Vagstadt, A.L.		Authors: Mordhorst, S., Badmann, T., Boesch, N.M., Morinaka, B.I., Rach, H., Piel, J., Groll, M., Vagstadt, A.L.

OCA: Protected "8brp" [edit=sysop:move=sysop]

OCA — Wed, 30 Nov 2022 08:28:19 GMT

Protected "8brp" [edit=sysop:move=sysop]

←Older revision

Revision as of 08:28, 30 November 2022

OCA: New page: '''Unreleased structure''' The entry 8brp is ON HOLD Authors: Mordhorst, S., Badmann, T., Boesch, N.M., Morinaka, B.I., Rach, H., Piel, J., Groll, M., Vagstadt, A.L. Description: Pepti...

OCA — Wed, 30 Nov 2022 08:28:15 GMT

New page: '''Unreleased structure''' The entry 8brp is ON HOLD Authors: Mordhorst, S., Badmann, T., Boesch, N.M., Morinaka, B.I., Rach, H., Piel, J., Groll, M., Vagstadt, A.L. Description: Pepti...

New page

'''Unreleased structure'''

The entry 8brp is ON HOLD

Authors: Mordhorst, S., Badmann, T., Boesch, N.M., Morinaka, B.I., Rach, H., Piel, J., Groll, M., Vagstadt, A.L.

Description: Peptide Arginase OspR from the cyanobacterium Kamptonema sp.
[[Category: Unreleased Structures]]
[[Category: Badmann, T]]
[[Category: Rach, H]]
[[Category: Morinaka, B.I]]
[[Category: Vagstadt, A.L]]
[[Category: Piel, J]]
[[Category: Groll, M]]
[[Category: Mordhorst, S]]
[[Category: Boesch, N.M]]

8brp - Revision history

OCA at 06:02, 2 March 2023

OCA at 05:28, 28 December 2022

OCA: Protected "8brp" [edit=sysop:move=sysop]

OCA: New page: '''Unreleased structure''' The entry 8brp is ON HOLD Authors: Mordhorst, S., Badmann, T., Boesch, N.M., Morinaka, B.I., Rach, H., Piel, J., Groll, M., Vagstadt, A.L. Description: Pepti...