OCA at 07:17, 12 July 2023

OCA — Wed, 12 Jul 2023 07:17:08 GMT

←Older revision		Revision as of 07:17, 12 July 2023
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-	'''Unreleased structure'''

-	~~The entry~~ 8bxd is ~~ON HOLD until Paper~~ Publication	+	==Alvinella pompejana nicotinic acetylcholine receptor Alpo4 in apo state (Alpo4_LMNG_Serotonin dataset 4)==
		+	<StructureSection load='8bxd' size='340' side='right'caption='[[8bxd]], [[Resolution\|resolution]] 6.20Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[8bxd]] is a 5 chain structure with sequence from [https://en.wikipedia.org/wiki/Alvinella_pompejana Alvinella pompejana]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8BXD OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8BXD FirstGlance]. <br>
		+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution\|Resolution]] 6.2Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8bxd FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8bxd OCA], [https://pdbe.org/8bxd PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8bxd RCSB], [https://www.ebi.ac.uk/pdbsum/8bxd PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8bxd ProSAT]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Cys-loop receptors or pentameric ligand-gated ion channels are mediators of electrochemical signaling throughout the animal kingdom. Because of their critical function in neurotransmission and high potential as drug targets, Cys-loop receptors from humans and closely related organisms have been thoroughly investigated, whereas molecular mechanisms of neurotransmission in invertebrates are less understood. When compared with vertebrates, the invertebrate genomes underwent a drastic expansion in the number of the nACh-like genes associated with receptors of unknown function. Understanding this diversity contributes to better insight into the evolution and possible functional divergence of these receptors. In this work, we studied orphan receptor Alpo4 from an extreme thermophile worm Alvinella pompejana. Sequence analysis points towards its remote relation to characterized nACh receptors. We solved the cryo-EM structure of the lophotrochozoan nACh-like receptor in which a CHAPS molecule is tightly bound to the orthosteric site. We show that the binding of CHAPS leads to extending of the loop C at the orthosteric site and a quaternary twist between extracellular and transmembrane domains. Both the ligand binding site and the channel pore reveal unique features. These include a conserved Trp residue in loop B of the ligand binding site which is flipped into an apparent self-liganded state in the apo structure. The ion pore of Alpo4 is tightly constricted by a ring of methionines near the extracellular entryway of the channel pore. Our data provide a structural basis for a functional understanding of Alpo4 and hints towards new strategies for designing specific channel modulators.

-	~~Authors:~~ De Gieter, S., ~~Efremov~~, ~~R.G.~~, ~~Ulens~~, C.	+	Sterol derivative binding to the orthosteric site causes conformational changes in an invertebrate Cys-loop receptor.,De Gieter S, Gallagher C, Wijckmans E, Pasini D, Ulens C, Efremov RG Elife. 2023 Jul 3;12:e86029. doi: 10.7554/eLife.86029. PMID:37395731<ref>PMID:37395731</ref>

-	~~Description~~: Alvinella pompejana ~~nicotinic acetylcholine receptor Alpo4 in apo state (Alpo4_LMNG_Serotonin dataset 4)~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased~~ Structures]]	+	</div>
-	[[Category: De Gieter, S]]	+	<div class="pdbe-citations 8bxd" style="background-color:#fffaf0;"></div>
-	[[Category: ~~Ulens, C~~]]	+	== References ==
-	[[Category: ~~Efremov, R.G~~]]	+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Alvinella pompejana]]
		+	[[Category: Large Structures]]
		+	[[Category: De Gieter S]]
		+	[[Category: Efremov RG]]
		+	[[Category: Ulens C]]

OCA at 04:54, 8 February 2023

OCA — Wed, 08 Feb 2023 04:54:28 GMT

←Older revision		Revision as of 04:54, 8 February 2023
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 8bxd is ON HOLD	+	The entry 8bxd is ON HOLD until Paper Publication

	Authors: De Gieter, S., Efremov, R.G., Ulens, C.		Authors: De Gieter, S., Efremov, R.G., Ulens, C.

OCA at 05:38, 11 January 2023

OCA — Wed, 11 Jan 2023 05:38:34 GMT

←Older revision		Revision as of 05:38, 11 January 2023
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 8bxd is ON HOLD ~~until sometime in the future~~	+	The entry 8bxd is ON HOLD

	Authors: De Gieter, S., Efremov, R.G., Ulens, C.		Authors: De Gieter, S., Efremov, R.G., Ulens, C.

OCA at 05:30, 28 December 2022

OCA — Wed, 28 Dec 2022 05:30:15 GMT

←Older revision		Revision as of 05:30, 28 December 2022
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 8bxd is ON HOLD	+	The entry 8bxd is ON HOLD until sometime in the future

	Authors: De Gieter, S., Efremov, R.G., Ulens, C.		Authors: De Gieter, S., Efremov, R.G., Ulens, C.

OCA: Protected "8bxd" [edit=sysop:move=sysop]

OCA — Wed, 14 Dec 2022 07:30:18 GMT

Protected "8bxd" [edit=sysop:move=sysop]

←Older revision

Revision as of 07:30, 14 December 2022

OCA: New page: '''Unreleased structure''' The entry 8bxd is ON HOLD Authors: De Gieter, S., Efremov, R.G., Ulens, C. Description: Alvinella pompejana nicotinic acetylcholine receptor Alpo4 in apo sta...

OCA — Wed, 14 Dec 2022 07:30:17 GMT

New page: '''Unreleased structure''' The entry 8bxd is ON HOLD Authors: De Gieter, S., Efremov, R.G., Ulens, C. Description: Alvinella pompejana nicotinic acetylcholine receptor Alpo4 in apo sta...

New page