OCA at 08:15, 14 June 2023

OCA — Wed, 14 Jun 2023 08:15:46 GMT

←Older revision		Revision as of 08:15, 14 June 2023
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-	'''Unreleased structure'''

-	~~The entry~~ 8c0j is ~~ON HOLD until Paper~~ Publication	+	==Structure of AmiB enzymatic domain bound to the EnvC LytM domain==
		+	<StructureSection load='8c0j' size='340' side='right'caption='[[8c0j]], [[Resolution\|resolution]] 3.38Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[8c0j]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Citrobacter_rodentium Citrobacter rodentium]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8C0J OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8C0J FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8c0j FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8c0j OCA], [https://pdbe.org/8c0j PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8c0j RCSB], [https://www.ebi.ac.uk/pdbsum/8c0j PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8c0j ProSAT]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/A0A482PQR2_CITRO A0A482PQR2_CITRO]
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	AmiA and AmiB are peptidoglycan-hydrolyzing enzymes from Escherichia coli that are required to break the peptidoglycan layer during bacterial cell division and maintain integrity of the cell envelope. In vivo, the activity of AmiA and AmiB is tightly controlled through their interactions with the membrane-bound FtsEX-EnvC complex. Activation of AmiA and AmiB requires access to a groove in the amidase-activating LytM domain of EnvC which is gated by ATP-driven conformational changes in FtsEX-EnvC complex. Here, we present a high-resolution structure of the isolated AmiA protein, confirming that it is autoinhibited in the same manner as AmiB and AmiC, and a complex of the AmiB enzymatic domain bound to the activating EnvC LytM domain. In isolation, the active site of AmiA is blocked by an autoinhibitory helix that binds directly to the catalytic zinc and fills the volume expected to accommodate peptidoglycan binding. In the complex, binding of the EnvC LytM domain induces a conformational change that displaces the amidase autoinhibitory helix and reorganizes the active site for activity. Our structures, together with complementary mutagenesis work, defines the conformational changes required to activate AmiA and/or AmiB through their interaction with their cognate activator EnvC.

-	~~Authors~~:	+	Activator-induced conformational changes regulate division-associated peptidoglycan amidases.,Cook J, Baverstock TC, McAndrew MBL, Roper DI, Stansfeld PJ, Crow A Proc Natl Acad Sci U S A. 2023 Jun 13;120(24):e2302580120. doi: , 10.1073/pnas.2302580120. Epub 2023 Jun 5. PMID:37276423<ref>PMID:37276423</ref>

-	~~Description~~:	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased~~ Structures]]	+	</div>
		+	<div class="pdbe-citations 8c0j" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Citrobacter rodentium]]
		+	[[Category: Large Structures]]
		+	[[Category: Crow A]]

OCA: Protected "8c0j" [edit=sysop:move=sysop]

OCA — Wed, 28 Dec 2022 05:32:24 GMT

Protected "8c0j" [edit=sysop:move=sysop]

←Older revision

Revision as of 05:32, 28 December 2022

OCA: New page: '''Unreleased structure''' The entry 8c0j is ON HOLD until Paper Publication Authors: Description: Category: Unreleased Structures

OCA — Wed, 28 Dec 2022 05:32:23 GMT

New page: '''Unreleased structure''' The entry 8c0j is ON HOLD until Paper Publication Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 8c0j is ON HOLD until Paper Publication

Authors:

Description:
[[Category: Unreleased Structures]]

8c0j - Revision history

OCA at 08:15, 14 June 2023

OCA: Protected "8c0j" [edit=sysop:move=sysop]

OCA: New page: '''Unreleased structure''' The entry 8c0j is ON HOLD until Paper Publication Authors: Description: Category: Unreleased Structures