8c0t - Revision history

OCA: Replacing page with ''''Unreleased structure''' The entry 8c0t is ON HOLD Authors: Basquin, J., Griesbeck, O., Valiente-Gabioud, A. Description: NRS 1.2: Fluorescent Sensors for Imaging Interst...'

OCA — Wed, 22 Nov 2023 05:27:21 GMT

Replacing page with ''''Unreleased structure''' The entry 8c0t is ON HOLD Authors: Basquin, J., Griesbeck, O., Valiente-Gabioud, A. Description: NRS 1.2: Fluorescent Sensors for Imaging Interst...'

←Older revision		Revision as of 05:27, 22 November 2023
Line 1:		Line 1:
		+	'''Unreleased structure'''

-	~~==NRS 1.2: Fluorescent Sensors for Imaging Interstitial Calcium==~~	+	The entry 8c0t is ON HOLD
-	~~<StructureSection load='~~8c0t~~' size='340' side='right'caption='[[8c0t]], [[Resolution\|resolution]] 1.28Å' scene=''>~~	+
-	~~== Structural highlights ==~~	+
-	~~<table><tr><td colspan='2'>[[8c0t]]~~ is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Branchiostoma_lanceolatum Branchiostoma lanceolatum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8C0T OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8C0T FirstGlance]. <br>	+
-	~~</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.28Å</td></tr>~~	+
-	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=CR2:{(4Z)-2-(AMINOMETHYL)-4-[(4-HYDROXYPHENYL)METHYLIDENE]-5-OXO-4,5-DIHYDRO-1H-IMIDAZOL-1-YL}ACETIC+ACID'>CR2</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>	+
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8c0t FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8c0t OCA], [https://pdbe.org/8c0t PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8c0t RCSB], [https://www.ebi.ac.uk/pdbsum/8c0t PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8c0t ProSAT]</span></td></tr>	+
-	~~</table>~~	+
-	~~<div style="background-color:#fffaf0;">~~	+
-	~~== Publication Abstract from PubMed ==~~	+
-	Calcium in interstitial fluids is central to systemic physiology and a crucial ion pool for entry into cells through numerous plasma membrane channels. Its study has been limited by the scarcity of methods that allow monitoring in tight inter-cell spaces of living tissues. Here we present high performance ultra-low affinity genetically encoded calcium biosensors named GreenT-ECs. GreenT-ECs combine large fluorescence changes upon calcium binding and binding affinities (Kds) ranging from 0.8 mM to 2.9 mM, making them tuned to calcium concentrations in extracellular organismal fluids. We validated GreenT-ECs in rodent hippocampal neurons and transgenic zebrafish in vivo, where the sensors enabled monitoring homeostatic regulation of tissue interstitial calcium. GreenT-ECs may become useful for recording very large calcium transients and for imaging calcium homeostasis in inter-cell structures in live tissues and organisms.	+

-	~~Fluorescent sensors for imaging of interstitial calcium.,Valiente-Gabioud AA, Garteizgogeascoa Suner I, Idziak A, Fabritius A,~~ Basquin J, ~~Angibaud~~ J, ~~Nagerl UV~~, ~~Singh SP, Griesbeck~~ O ~~Nat Commun~~. ~~2023 Oct 5;14(1):6220. doi: 10.1038/s41467~~-~~023-41928-w~~. ~~PMID:37798285<ref>PMID:37798285</ref>~~	+	Authors: Basquin, J., Griesbeck, O., Valiente-Gabioud, A.

-	~~From MEDLINE®/PubMed®, a database of the U~~.~~S. National Library of Medicine.<br>~~	+	Description: NRS 1.2: Fluorescent Sensors for Imaging Interstitial Calcium
-	~~</div>~~	+	[[Category: Unreleased Structures]]
-	~~<div class="pdbe-citations 8c0t" style="background-color~~:~~#fffaf0;"></div>~~	+	[[Category: Valiente-Gabioud, A]]
-	~~== References ==~~	+	[[Category: Griesbeck, O]]
-	~~<references/>~~	+	[[Category: Basquin, J]]
-	~~__TOC__~~	+
-	~~</StructureSection>~~	+
-	[[Category: ~~Branchiostoma lanceolatum]]~~	+
-	~~[[Category: Large~~ Structures]]	+
-	[[Category: ~~Basquin J~~]]	+
-	[[Category: Griesbeck O]]	+
-	[[Category: ~~Valiente-Gabioud A~~]]	+

OCA at 07:31, 15 November 2023

OCA — Wed, 15 Nov 2023 07:31:24 GMT

←Older revision		Revision as of 07:31, 15 November 2023
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 8c0t is ~~ON HOLD~~	+	==NRS 1.2: Fluorescent Sensors for Imaging Interstitial Calcium==
		+	<StructureSection load='8c0t' size='340' side='right'caption='[[8c0t]], [[Resolution\|resolution]] 1.28Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[8c0t]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Branchiostoma_lanceolatum Branchiostoma lanceolatum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8C0T OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8C0T FirstGlance]. <br>
		+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.28Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=CR2:{(4Z)-2-(AMINOMETHYL)-4-[(4-HYDROXYPHENYL)METHYLIDENE]-5-OXO-4,5-DIHYDRO-1H-IMIDAZOL-1-YL}ACETIC+ACID'>CR2</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8c0t FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8c0t OCA], [https://pdbe.org/8c0t PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8c0t RCSB], [https://www.ebi.ac.uk/pdbsum/8c0t PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8c0t ProSAT]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Calcium in interstitial fluids is central to systemic physiology and a crucial ion pool for entry into cells through numerous plasma membrane channels. Its study has been limited by the scarcity of methods that allow monitoring in tight inter-cell spaces of living tissues. Here we present high performance ultra-low affinity genetically encoded calcium biosensors named GreenT-ECs. GreenT-ECs combine large fluorescence changes upon calcium binding and binding affinities (Kds) ranging from 0.8 mM to 2.9 mM, making them tuned to calcium concentrations in extracellular organismal fluids. We validated GreenT-ECs in rodent hippocampal neurons and transgenic zebrafish in vivo, where the sensors enabled monitoring homeostatic regulation of tissue interstitial calcium. GreenT-ECs may become useful for recording very large calcium transients and for imaging calcium homeostasis in inter-cell structures in live tissues and organisms.

-	~~Authors:~~ Basquin, J., ~~Griesbeck~~, O.~~, Valiente~~-~~Gabioud, A~~.	+	Fluorescent sensors for imaging of interstitial calcium.,Valiente-Gabioud AA, Garteizgogeascoa Suner I, Idziak A, Fabritius A, Basquin J, Angibaud J, Nagerl UV, Singh SP, Griesbeck O Nat Commun. 2023 Oct 5;14(1):6220. doi: 10.1038/s41467-023-41928-w. PMID:37798285<ref>PMID:37798285</ref>

-	~~Description: NRS 1~~.2: ~~Fluorescent Sensors for Imaging Interstitial Calcium~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased~~ Structures]]	+	</div>
-	[[Category: Basquin, J]]	+	<div class="pdbe-citations 8c0t" style="background-color:#fffaf0;"></div>
-	[[Category: ~~Valiente-Gabioud, A~~]]	+	== References ==
-	[[Category: ~~Griesbeck, O~~]]	+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Branchiostoma lanceolatum]]
		+	[[Category: Large Structures]]
		+	[[Category: Basquin J]]
		+	[[Category: Griesbeck O]]
		+	[[Category: Valiente-Gabioud A]]

OCA at 12:01, 18 October 2023

OCA — Wed, 18 Oct 2023 12:01:21 GMT

←Older revision		Revision as of 12:01, 18 October 2023
Line 5:		Line 5:
	Authors: Basquin, J., Griesbeck, O., Valiente-Gabioud, A.		Authors: Basquin, J., Griesbeck, O., Valiente-Gabioud, A.

-	Description: ~~GreenT-ECs~~ 1.2: Fluorescent Sensors for Imaging Interstitial Calcium	+	Description: NRS 1.2: Fluorescent Sensors for Imaging Interstitial Calcium
	[[Category: Unreleased Structures]]		[[Category: Unreleased Structures]]
		+	[[Category: Basquin, J]]
	[[Category: Valiente-Gabioud, A]]		[[Category: Valiente-Gabioud, A]]
	[[Category: Griesbeck, O]]		[[Category: Griesbeck, O]]
-	[[Category: Basquin, J]]

OCA at 09:06, 1 February 2023

OCA — Wed, 01 Feb 2023 09:06:13 GMT

←Older revision		Revision as of 09:06, 1 February 2023
Line 5:		Line 5:
	Authors: Basquin, J., Griesbeck, O., Valiente-Gabioud, A.		Authors: Basquin, J., Griesbeck, O., Valiente-Gabioud, A.

-	Description: GreenT-ECs: Fluorescent Sensors for Imaging Interstitial Calcium	+	Description: GreenT-ECs 1.2: Fluorescent Sensors for Imaging Interstitial Calcium
	[[Category: Unreleased Structures]]		[[Category: Unreleased Structures]]
	[[Category: Valiente-Gabioud, A]]		[[Category: Valiente-Gabioud, A]]
	[[Category: Griesbeck, O]]		[[Category: Griesbeck, O]]
	[[Category: Basquin, J]]		[[Category: Basquin, J]]

OCA at 05:39, 11 January 2023

OCA — Wed, 11 Jan 2023 05:39:33 GMT

←Older revision		Revision as of 05:39, 11 January 2023
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 8c0t is ON HOLD ~~until sometime in the future~~	+	The entry 8c0t is ON HOLD

	Authors: Basquin, J., Griesbeck, O., Valiente-Gabioud, A.		Authors: Basquin, J., Griesbeck, O., Valiente-Gabioud, A.
Line 7:		Line 7:
	Description: GreenT-ECs: Fluorescent Sensors for Imaging Interstitial Calcium		Description: GreenT-ECs: Fluorescent Sensors for Imaging Interstitial Calcium
	[[Category: Unreleased Structures]]		[[Category: Unreleased Structures]]
-	[[Category: Basquin, J]]
	[[Category: Valiente-Gabioud, A]]		[[Category: Valiente-Gabioud, A]]
	[[Category: Griesbeck, O]]		[[Category: Griesbeck, O]]
		+	[[Category: Basquin, J]]

OCA: Protected "8c0t" [edit=sysop:move=sysop]

OCA — Wed, 28 Dec 2022 05:32:50 GMT

Protected "8c0t" [edit=sysop:move=sysop]

←Older revision

Revision as of 05:32, 28 December 2022

OCA: New page: '''Unreleased structure''' The entry 8c0t is ON HOLD until sometime in the future Authors: Basquin, J., Griesbeck, O., Valiente-Gabioud, A. Description: GreenT-ECs: Fluorescent Sensors...

OCA — Wed, 28 Dec 2022 05:32:49 GMT

New page: '''Unreleased structure''' The entry 8c0t is ON HOLD until sometime in the future Authors: Basquin, J., Griesbeck, O., Valiente-Gabioud, A. Description: GreenT-ECs: Fluorescent Sensors...

New page

'''Unreleased structure'''

The entry 8c0t is ON HOLD until sometime in the future

Authors: Basquin, J., Griesbeck, O., Valiente-Gabioud, A.

Description: GreenT-ECs: Fluorescent Sensors for Imaging Interstitial Calcium
[[Category: Unreleased Structures]]
[[Category: Basquin, J]]
[[Category: Valiente-Gabioud, A]]
[[Category: Griesbeck, O]]