OCA at 11:38, 13 December 2023

OCA — Wed, 13 Dec 2023 11:38:26 GMT

←Older revision		Revision as of 11:38, 13 December 2023
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 8c4d is ~~ON HOLD until Paper~~ Publication	+	==N-acetylmuramoyl-L-alanine amidase from Enterococcus faecium prophage genome==
		+	<StructureSection load='8c4d' size='340' side='right'caption='[[8c4d]], [[Resolution\|resolution]] 1.97Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[8c4d]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Enterococcus_faecium Enterococcus faecium]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8C4D OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8C4D FirstGlance]. <br>
		+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.97Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=FLC:CITRATE+ANION'>FLC</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8c4d FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8c4d OCA], [https://pdbe.org/8c4d PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8c4d RCSB], [https://www.ebi.ac.uk/pdbsum/8c4d PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8c4d ProSAT]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Multidrug-resistant (MDR) bacteria have become a growing threat to public health. The gram-positive Enterococcus faecium is classified by WHO as a high-priority pathogen among the global priority list of antibiotic-resistant bacteria. Peptidoglycan-degrading enzymes (PDEs), also known as enzybiotics, are useful bactericidal agents in the fight against resistant bacteria. In this work, a genome-based screening approach of the genome of E. faecium allowed the identification of a putative PDE gene with predictive amidase activity (EfAmi1; EC 3.5.1.28) in a prophage-integrated sequence. EfAmi1 is composed by two domains: a N-terminal Zn(2+)-dependent N-acetylmuramoyl-L-alanine amidase-2 (NALAA-2) domain and a C-terminal domain with unknown structure and function. The full-length gene of EfAmi1 was cloned and expressed as a 6xHis-tagged protein in E. coli. EfAmi1 was produced as a soluble protein, purified, and its lytic and antimicrobial activities were investigated using turbidity reduction and Kirby-Bauer disk-diffusion assays against clinically isolated bacterial pathogens. The crystal structure of the N-terminal amidase-2 domain was determined using X-ray crystallography at 1.97 A resolution. It adopts a globular fold with several alpha-helices surrounding a central five-stranded beta-sheet. Sequence comparison revealed a cluster of conserved amino acids that defines a putative binding site for a buried zinc ion. The results of the present study suggest that EfAmi1 displays high lytic and antimicrobial activity and may represent a promising new antimicrobial in the post-antibiotic era.

-	~~Authors: Papageorgiou, A.C~~., Premetis, ~~G.E.~~, ~~Labrou~~, N.E.	+	Structural and functional features of a broad-spectrum prophage-encoded enzybiotic from Enterococcus faecium.,Premetis GE, Stathi A, Papageorgiou AC, Labrou NE Sci Rep. 2023 May 8;13(1):7450. doi: 10.1038/s41598-023-34309-2. PMID:37156923<ref>PMID:37156923</ref>

-	~~Description: N~~-~~acetylmuramoyl~~-~~L-alanine amidase from~~ Enterococcus faecium ~~prophage genome~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased~~ Structures]]	+	</div>
-	[[Category: ~~Papageorgiou, A.C~~]]	+	<div class="pdbe-citations 8c4d" style="background-color:#fffaf0;"></div>
-	[[Category: ~~Premetis, G.E~~]]	+	== References ==
-	[[Category: ~~Labrou, N.E~~]]	+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Enterococcus faecium]]
		+	[[Category: Large Structures]]
		+	[[Category: Labrou NE]]
		+	[[Category: Papageorgiou AC]]
		+	[[Category: Premetis GE]]

OCA at 09:08, 1 February 2023

OCA — Wed, 01 Feb 2023 09:08:04 GMT

←Older revision		Revision as of 09:08, 1 February 2023
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 8c4d is ON HOLD	+	The entry 8c4d is ON HOLD until Paper Publication

	Authors: Papageorgiou, A.C., Premetis, G.E., Labrou, N.E.		Authors: Papageorgiou, A.C., Premetis, G.E., Labrou, N.E.
Line 8:		Line 8:
	[[Category: Unreleased Structures]]		[[Category: Unreleased Structures]]
	[[Category: Papageorgiou, A.C]]		[[Category: Papageorgiou, A.C]]
-	[[Category: Labrou, N.E]]
	[[Category: Premetis, G.E]]		[[Category: Premetis, G.E]]
		+	[[Category: Labrou, N.E]]

OCA: Protected "8c4d" [edit=sysop:move=sysop]

OCA — Wed, 11 Jan 2023 05:43:51 GMT

Protected "8c4d" [edit=sysop:move=sysop]

←Older revision

Revision as of 05:43, 11 January 2023

OCA: New page: '''Unreleased structure''' The entry 8c4d is ON HOLD Authors: Papageorgiou, A.C., Premetis, G.E., Labrou, N.E. Description: N-acetylmuramoyl-L-alanine amidase from Enterococcus faecium...

OCA — Wed, 11 Jan 2023 05:43:50 GMT

New page: '''Unreleased structure''' The entry 8c4d is ON HOLD Authors: Papageorgiou, A.C., Premetis, G.E., Labrou, N.E. Description: N-acetylmuramoyl-L-alanine amidase from Enterococcus faecium...

New page

'''Unreleased structure'''

The entry 8c4d is ON HOLD

Authors: Papageorgiou, A.C., Premetis, G.E., Labrou, N.E.

Description: N-acetylmuramoyl-L-alanine amidase from Enterococcus faecium prophage genome
[[Category: Unreleased Structures]]
[[Category: Papageorgiou, A.C]]
[[Category: Labrou, N.E]]
[[Category: Premetis, G.E]]

8c4d - Revision history

OCA at 11:38, 13 December 2023

OCA at 09:08, 1 February 2023

OCA: Protected "8c4d" [edit=sysop:move=sysop]

OCA: New page: '''Unreleased structure''' The entry 8c4d is ON HOLD Authors: Papageorgiou, A.C., Premetis, G.E., Labrou, N.E. Description: N-acetylmuramoyl-L-alanine amidase from Enterococcus faecium...