OCA at 06:52, 10 May 2023

OCA — Wed, 10 May 2023 06:52:03 GMT

←Older revision		Revision as of 06:52, 10 May 2023
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 8c5z is ~~ON HOLD~~	+	==RPA tetrameric supercomplex with AROD-OB-1==
		+	<StructureSection load='8c5z' size='340' side='right'caption='[[8c5z]], [[Resolution\|resolution]] 3.80Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[8c5z]] is a 12 chain structure with sequence from [https://en.wikipedia.org/wiki/Pyrococcus_abyssi Pyrococcus abyssi]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8C5Z OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8C5Z FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8c5z FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8c5z OCA], [https://pdbe.org/8c5z PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8c5z RCSB], [https://www.ebi.ac.uk/pdbsum/8c5z PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8c5z ProSAT]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/G8ZHS0_PYRAB G8ZHS0_PYRAB]
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Replication Protein A (RPA) is a heterotrimeric single stranded DNA-binding protein with essential roles in DNA replication, recombination and repair. Little is known about the structure of RPA in Archaea, the third domain of life. By using an integrative structural, biochemical and biophysical approach, we extensively characterize RPA from Pyrococcus abyssi in the presence and absence of DNA. The obtained X-ray and cryo-EM structures reveal that the trimerization core and interactions promoting RPA clustering on ssDNA are shared between archaea and eukaryotes. However, we also identified a helical domain named AROD (Acidic Rpa1 OB-binding Domain), and showed that, in Archaea, RPA forms an unanticipated tetrameric supercomplex in the absence of DNA. The four RPA molecules clustered within the tetramer could efficiently coat and protect stretches of ssDNA created by the advancing replisome. Finally, our results provide insights into the evolution of this primordial replication factor in eukaryotes.

-	~~Authors~~:	+	DNA-binding mechanism and evolution of replication protein A.,Madru C, Martinez-Carranza M, Laurent S, Alberti AC, Chevreuil M, Raynal B, Haouz A, Le Meur RA, Delarue M, Henneke G, Flament D, Krupovic M, Legrand P, Sauguet L Nat Commun. 2023 Apr 22;14(1):2326. doi: 10.1038/s41467-023-38048-w. PMID:37087464<ref>PMID:37087464</ref>

-	~~Description~~:	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased~~ Structures]]	+	</div>
		+	<div class="pdbe-citations 8c5z" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Pyrococcus abyssi]]
		+	[[Category: Legrand P]]
		+	[[Category: Madru C]]
		+	[[Category: Martinez-Carranza M]]
		+	[[Category: Sauguet L]]

OCA: Protected "8c5z" [edit=sysop:move=sysop]

OCA — Wed, 18 Jan 2023 04:56:36 GMT

Protected "8c5z" [edit=sysop:move=sysop]

←Older revision

Revision as of 04:56, 18 January 2023

OCA: New page: '''Unreleased structure''' The entry 8c5z is ON HOLD Authors: Description: Category: Unreleased Structures

OCA — Wed, 18 Jan 2023 04:56:35 GMT

New page: '''Unreleased structure''' The entry 8c5z is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 8c5z is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]

8c5z - Revision history

OCA at 06:52, 10 May 2023

OCA: Protected "8c5z" [edit=sysop:move=sysop]

OCA: New page: '''Unreleased structure''' The entry 8c5z is ON HOLD Authors: Description: Category: Unreleased Structures