OCA at 06:03, 2 March 2023

OCA — Thu, 02 Mar 2023 06:03:01 GMT

←Older revision		Revision as of 06:03, 2 March 2023
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-	'''Unreleased structure'''

-	~~The entry~~ 8c8q is ~~ON HOLD until Paper~~ Publication	+	==Cytochrome c oxidase from Schizosaccharomyces pombe==
		+	<StructureSection load='8c8q' size='340' side='right'caption='[[8c8q]], [[Resolution\|resolution]] 3.36Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[8c8q]] is a 10 chain structure with sequence from [https://en.wikipedia.org/wiki/Schizosaccharomyces_pombe Schizosaccharomyces pombe]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8C8Q OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8C8Q FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=CU:COPPER+(II)+ION'>CU</scene>, <scene name='pdbligand=CUA:DINUCLEAR+COPPER+ION'>CUA</scene>, <scene name='pdbligand=HEA:HEME-A'>HEA</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=PEF:DI-PALMITOYL-3-SN-PHOSPHATIDYLETHANOLAMINE'>PEF</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8c8q FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8c8q OCA], [https://pdbe.org/8c8q PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8c8q RCSB], [https://www.ebi.ac.uk/pdbsum/8c8q PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8c8q ProSAT]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/COX1_SCHPO COX1_SCHPO] Component of the cytochrome c oxidase, the last enzyme in the mitochondrial electron transport chain which drives oxidative phosphorylation. The respiratory chain contains 3 multisubunit complexes succinate dehydrogenase (complex II, CII), ubiquinol-cytochrome c oxidoreductase (cytochrome b-c1 complex, complex III, CIII) and cytochrome c oxidase (complex IV, CIV), that cooperate to transfer electrons derived from NADH and succinate to molecular oxygen, creating an electrochemical gradient over the inner membrane that drives transmembrane transport and the ATP synthase. Cytochrome c oxidase is the component of the respiratory chain that catalyzes the reduction of oxygen to water. Electrons originating from reduced cytochrome c in the intermembrane space (IMS) are transferred via the dinuclear copper A center (CU(A)) of subunit 2 and heme A of subunit 1 to the active site in subunit 1, a binuclear center (BNC) formed by heme A3 and copper B (CU(B)). The BNC reduces molecular oxygen to 2 water molecules using 4 electrons from cytochrome c in the IMS and 4 protons from the mitochondrial matrix.[UniProtKB:P00401]
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Fission yeast Schizosaccharomyces pombe serves as model organism for studying higher eukaryotes. We combined the use of cryo-EM and spectroscopy to investigate the structure and function of affinity purified respiratory complex IV (CIV) from S. pombe. The reaction sequence of the reduced enzyme with O(2) proceeds over a time scale of micros-ms, similar to that of the mammalian CIV. The cryo-EM structure of CIV revealed eleven subunits as well as a bound hypoxia-induced gene 1 (Hig1) domain of respiratory supercomplex factor 2 (Rcf2). These results suggest that binding of Rcf2 does not require the presence of a CIII-CIV supercomplex, i.e. Rcf2 is a component of CIV. An AlphaFold-Multimer model suggests that the Hig1 domains of both Rcf1 and Rcf2 bind at the same site of CIV suggesting that their binding is mutually exclusive. Furthermore, the differential functional effect of Rcf1 or Rcf2 is presumably caused by interactions of CIV with their different non-Hig1 domain parts.

-	~~Authors: Moe~~, A., Adelroth, P., Brzezinski, P., Nasvik Ojemyr, L.	+	Cryo-EM structure and function of S. pombe complex IV with bound respiratory supercomplex factor.,Moe A, Adelroth P, Brzezinski P, Nasvik Ojemyr L Commun Chem. 2023 Feb 16;6(1):32. doi: 10.1038/s42004-023-00827-3. PMID:36797353<ref>PMID:36797353</ref>

-	~~Description~~: ~~Cytochrome c oxidase from Schizosaccharomyces pombe~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased~~ Structures]]	+	</div>
-	[[Category: ~~Moe, A~~]]	+	<div class="pdbe-citations 8c8q" style="background-color:#fffaf0;"></div>
-	[[Category: ~~Nasvik Ojemyr, L~~]]	+	== References ==
-	[[Category: Brzezinski, P]]	+	<references/>
-	[[Category: ~~Adelroth, P~~]]	+	__TOC__
		+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Schizosaccharomyces pombe]]
		+	[[Category: Adelroth P]]
		+	[[Category: Brzezinski P]]
		+	[[Category: Moe A]]
		+	[[Category: Nasvik Ojemyr L]]

OCA at 04:57, 8 February 2023

OCA — Wed, 08 Feb 2023 04:57:11 GMT

←Older revision		Revision as of 04:57, 8 February 2023
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 8c8q is ON HOLD	+	The entry 8c8q is ON HOLD until Paper Publication

	Authors: Moe, A., Adelroth, P., Brzezinski, P., Nasvik Ojemyr, L.		Authors: Moe, A., Adelroth, P., Brzezinski, P., Nasvik Ojemyr, L.

OCA: Protected "8c8q" [edit=sysop:move=sysop]

OCA — Wed, 01 Feb 2023 09:09:45 GMT

Protected "8c8q" [edit=sysop:move=sysop]

←Older revision

Revision as of 09:09, 1 February 2023

OCA: New page: '''Unreleased structure''' The entry 8c8q is ON HOLD Authors: Moe, A., Adelroth, P., Brzezinski, P., Nasvik Ojemyr, L. Description: Cytochrome c oxidase from Schizosaccharomyces pombe ...

OCA — Wed, 01 Feb 2023 09:09:42 GMT

New page: '''Unreleased structure''' The entry 8c8q is ON HOLD Authors: Moe, A., Adelroth, P., Brzezinski, P., Nasvik Ojemyr, L. Description: Cytochrome c oxidase from Schizosaccharomyces pombe ...

New page

'''Unreleased structure'''

The entry 8c8q is ON HOLD

Authors: Moe, A., Adelroth, P., Brzezinski, P., Nasvik Ojemyr, L.

Description: Cytochrome c oxidase from Schizosaccharomyces pombe
[[Category: Unreleased Structures]]
[[Category: Moe, A]]
[[Category: Nasvik Ojemyr, L]]
[[Category: Brzezinski, P]]
[[Category: Adelroth, P]]

8c8q - Revision history

OCA at 06:03, 2 March 2023

OCA at 04:57, 8 February 2023

OCA: Protected "8c8q" [edit=sysop:move=sysop]

OCA: New page: '''Unreleased structure''' The entry 8c8q is ON HOLD Authors: Moe, A., Adelroth, P., Brzezinski, P., Nasvik Ojemyr, L. Description: Cytochrome c oxidase from Schizosaccharomyces pombe ...