OCA at 17:05, 26 April 2023

OCA — Wed, 26 Apr 2023 17:05:02 GMT

←Older revision		Revision as of 17:05, 26 April 2023
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 8cky is ~~ON HOLD until Paper~~ Publication	+	==HIV-1 mature capsid hexamer from CA-IP6 CLPs, bound to Nup153 peptide==
		+	<StructureSection load='8cky' size='340' side='right'caption='[[8cky]], [[Resolution\|resolution]] 2.60Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[8cky]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [https://en.wikipedia.org/wiki/Human_immunodeficiency_virus_1 Human immunodeficiency virus 1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8CKY OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8CKY FirstGlance]. <br>
		+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8cky FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8cky OCA], [https://pdbe.org/8cky PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8cky RCSB], [https://www.ebi.ac.uk/pdbsum/8cky PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8cky ProSAT]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/B6DRA0_9HIV1 B6DRA0_9HIV1]
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The mature HIV-1 capsid protects the viral genome and interacts with host proteins to travel from the cell periphery into the nucleus. To achieve this, the capsid protein, CA, constructs conical capsids from a lattice of hexamers and pentamers, and engages in and then relinquishes multiple interactions with cellular proteins in an orchestrated fashion. Cellular host factors including Nup153, CPSF6, and Sec24C engage the same pocket within CA hexamers. How CA assembles pentamers and hexamers of different curvatures, how CA oligomerization states or curvature might modulate host-protein interactions, and how binding of multiple cofactors to a single site is coordinated, all remain to be elucidated. Here, using single-particle cryoEM, we have determined the structure of the mature HIV-1 CA pentamer and hexamer from conical CA-IP(6) polyhedra to ~3 A resolution. We also determined structures of hexamers in the context of multiple lattice curvatures and number of pentamer contacts. Comparison of these structures, bound or not to host protein peptides, revealed two structural switches within HIV-1 CA that modulate peptide binding according to CA lattice curvature and whether CA is hexameric or pentameric. These observations suggest that the conical HIV-1 capsid has different host-protein binding properties at different positions on its surface, which may facilitate cell entry and represent an evolutionary advantage of conical morphology.

-	~~Authors:~~ Stacey, J.~~C.V~~., ~~Briggs, J~~.A.G.	+	Two structural switches in HIV-1 capsid regulate capsid curvature and host factor binding.,Stacey JCV, Tan A, Lu JM, James LC, Dick RA, Briggs JAG Proc Natl Acad Sci U S A. 2023 Apr 18;120(16):e2220557120. doi: , 10.1073/pnas.2220557120. Epub 2023 Apr 11. PMID:37040417<ref>PMID:37040417</ref>

-	~~Description: HIV~~-~~1 mature capsid hexamer from CA~~-~~IP6 CLPs, bound to Nup153 peptide~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased~~ Structures]]	+	</div>
-	[[Category: Briggs~~, J.A.G~~]]	+	<div class="pdbe-citations 8cky" style="background-color:#fffaf0;"></div>
-	[[Category: Stacey~~, J.C.V~~]]	+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Homo sapiens]]
		+	[[Category: Human immunodeficiency virus 1]]
		+	[[Category: Large Structures]]
		+	[[Category: Briggs JAG]]
		+	[[Category: Stacey JCV]]

OCA at 18:35, 12 April 2023

OCA — Wed, 12 Apr 2023 18:35:28 GMT

←Older revision		Revision as of 18:35, 12 April 2023
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 8cky is ON HOLD	+	The entry 8cky is ON HOLD until Paper Publication

	Authors: Stacey, J.C.V., Briggs, J.A.G.		Authors: Stacey, J.C.V., Briggs, J.A.G.

OCA: Protected "8cky" [edit=sysop:move=sysop]

OCA — Wed, 22 Feb 2023 09:51:06 GMT

Protected "8cky" [edit=sysop:move=sysop]

←Older revision

Revision as of 09:51, 22 February 2023

OCA: New page: '''Unreleased structure''' The entry 8cky is ON HOLD Authors: Stacey, J.C.V., Briggs, J.A.G. Description: HIV-1 mature capsid hexamer from CA-IP6 CLPs, bound to Nup153 peptide [[Catego...

OCA — Wed, 22 Feb 2023 09:51:02 GMT

New page: '''Unreleased structure''' The entry 8cky is ON HOLD Authors: Stacey, J.C.V., Briggs, J.A.G. Description: HIV-1 mature capsid hexamer from CA-IP6 CLPs, bound to Nup153 peptide [[Catego...

New page

'''Unreleased structure'''

The entry 8cky is ON HOLD

Authors: Stacey, J.C.V., Briggs, J.A.G.

Description: HIV-1 mature capsid hexamer from CA-IP6 CLPs, bound to Nup153 peptide
[[Category: Unreleased Structures]]
[[Category: Briggs, J.A.G]]
[[Category: Stacey, J.C.V]]

8cky - Revision history

OCA at 17:05, 26 April 2023

OCA at 18:35, 12 April 2023

OCA: Protected "8cky" [edit=sysop:move=sysop]

OCA: New page: '''Unreleased structure''' The entry 8cky is ON HOLD Authors: Stacey, J.C.V., Briggs, J.A.G. Description: HIV-1 mature capsid hexamer from CA-IP6 CLPs, bound to Nup153 peptide [[Catego...