OCA at 09:07, 30 August 2023

OCA — Wed, 30 Aug 2023 09:07:19 GMT

←Older revision		Revision as of 09:07, 30 August 2023
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 8cq6 is ~~ON HOLD until Paper~~ Publication	+	==Bifunctional cyclohexadienyl dehydratase/chorismate mutase from Duganella sacchari==
		+	<StructureSection load='8cq6' size='340' side='right'caption='[[8cq6]], [[Resolution\|resolution]] 2.44Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[8cq6]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Duganella_sacchari Duganella sacchari]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8CQ6 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8CQ6 FirstGlance]. <br>
		+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.44Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8cq6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8cq6 OCA], [https://pdbe.org/8cq6 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8cq6 RCSB], [https://www.ebi.ac.uk/pdbsum/8cq6 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8cq6 ProSAT]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/A0A1M7QNQ8_9BURK A0A1M7QNQ8_9BURK]
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Chorismate mutase (CM) and cyclohexadienyl dehydratase (CDT) catalyze two subsequent reactions in the intracellular biosynthesis of l-phenylalanine (Phe). Here, we report the discovery of novel and extremely rare bifunctional fusion enzymes, consisting of fused CM and CDT domains, which are exported from the cytoplasm. Such enzymes were found in only nine bacterial species belonging to non-pathogenic gamma- or beta-Proteobacteria. In gamma-proteobacterial fusion enzymes, the CM domain is N-terminal to the CDT domain, whereas in beta-Proteobacteria the order is inverted. The CM domains share 15-20% sequence identity with the AroQ(gamma) class CM holotype of Mycobacterium tuberculosis (MtCM), and the CDT domains 40-60% identity with the exported monofunctional enzyme of Pseudomonas aeruginosa (PheC). In vitro kinetics revealed a K(m) <7 muM, much lower than for MtCM, whereas kinetic parameters are similar for CDT domains and PheC. There is no feedback inhibition of CM or CDT by the pathway's end product Phe, and no catalytic benefit of the domain fusion compared to engineered single-domain constructs. The fusion enzymes of Aequoribacter fuscus, Janthinobacterium sp. HH01, and Duganella sacchari were crystallized and their structures refined to 1.6, 1.7, and 2.4 A resolution, respectively. Neither the crystal structures nor size-exclusion chromatography show evidence for substrate channeling or higher oligomeric structure that could account for cooperation of CM and CDT active sites. The genetic neighborhood with genes encoding transporter and substrate binding proteins suggests that these exported bifunctional fusion enzymes may participate in signaling systems rather than in the biosynthesis of Phe.

-	~~Authors~~: ~~Khatanbaatar~~, T., ~~Cordara~~, G., Krengel, U.	+	Novel exported fusion enzymes with chorismate mutase and cyclohexadienyl dehydratase activity: shikimate pathway enzymes teamed up in no man's land.,Stocker C, Khatanbaatar T, Bressan L, Wurth-Roderer K, Cordara G, Krengel U, Kast P J Biol Chem. 2023 Aug 14:105161. doi: 10.1016/j.jbc.2023.105161. PMID:37586588<ref>PMID:37586588</ref>

-	~~Description~~: ~~Bifunctional cyclohexadienyl dehydratase~~/~~chorismate mutase from Duganella sacchari~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased Structures~~]]	+	</div>
-	[[Category: ~~Krengel, U~~]]	+	<div class="pdbe-citations 8cq6" style="background-color:#fffaf0;"></div>
-	[[Category: Cordara, G]]	+	== References ==
-	[[Category: Khatanbaatar, T]]	+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Duganella sacchari]]
		+	[[Category: Large Structures]]
		+	[[Category: Cordara G]]
		+	[[Category: Khatanbaatar T]]
		+	[[Category: Krengel U]]

OCA at 04:51, 7 April 2023

OCA — Fri, 07 Apr 2023 04:51:01 GMT

←Older revision		Revision as of 04:51, 7 April 2023
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 8cq6 is ON HOLD	+	The entry 8cq6 is ON HOLD until Paper Publication

	Authors: Khatanbaatar, T., Cordara, G., Krengel, U.		Authors: Khatanbaatar, T., Cordara, G., Krengel, U.
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	Description: Bifunctional cyclohexadienyl dehydratase/chorismate mutase from Duganella sacchari		Description: Bifunctional cyclohexadienyl dehydratase/chorismate mutase from Duganella sacchari
	[[Category: Unreleased Structures]]		[[Category: Unreleased Structures]]
-	[[Category: Cordara, G]]
	[[Category: Krengel, U]]		[[Category: Krengel, U]]
		+	[[Category: Cordara, G]]
	[[Category: Khatanbaatar, T]]		[[Category: Khatanbaatar, T]]

OCA: Protected "8cq6" [edit=sysop:move=sysop]

OCA — Wed, 15 Mar 2023 07:32:36 GMT

Protected "8cq6" [edit=sysop:move=sysop]

←Older revision

Revision as of 07:32, 15 March 2023

OCA: New page: '''Unreleased structure''' The entry 8cq6 is ON HOLD Authors: Khatanbaatar, T., Cordara, G., Krengel, U. Description: Bifunctional cyclohexadienyl dehydratase/chorismate mutase from Du...

OCA — Wed, 15 Mar 2023 07:32:35 GMT

New page: '''Unreleased structure''' The entry 8cq6 is ON HOLD Authors: Khatanbaatar, T., Cordara, G., Krengel, U. Description: Bifunctional cyclohexadienyl dehydratase/chorismate mutase from Du...

New page

'''Unreleased structure'''

The entry 8cq6 is ON HOLD

Authors: Khatanbaatar, T., Cordara, G., Krengel, U.

Description: Bifunctional cyclohexadienyl dehydratase/chorismate mutase from Duganella sacchari
[[Category: Unreleased Structures]]
[[Category: Cordara, G]]
[[Category: Krengel, U]]
[[Category: Khatanbaatar, T]]

8cq6 - Revision history

OCA at 09:07, 30 August 2023

OCA at 04:51, 7 April 2023

OCA: Protected "8cq6" [edit=sysop:move=sysop]

OCA: New page: '''Unreleased structure''' The entry 8cq6 is ON HOLD Authors: Khatanbaatar, T., Cordara, G., Krengel, U. Description: Bifunctional cyclohexadienyl dehydratase/chorismate mutase from Du...