OCA at 07:27, 22 November 2023

OCA — Wed, 22 Nov 2023 07:27:25 GMT

←Older revision		Revision as of 07:27, 22 November 2023
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 8crb is ~~ON HOLD until 2025~~-03-08	+	==Cryo-EM structure of PcrV/Fab(11-E5)==
		+	<StructureSection load='8crb' size='340' side='right'caption='[[8crb]], [[Resolution\|resolution]] 4.60Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[8crb]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [https://en.wikipedia.org/wiki/Pseudomonas_aeruginosa Pseudomonas aeruginosa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8CRB OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8CRB FirstGlance]. <br>
		+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution\|Resolution]] 4.6Å</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8crb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8crb OCA], [https://pdbe.org/8crb PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8crb RCSB], [https://www.ebi.ac.uk/pdbsum/8crb PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8crb ProSAT]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/MALE_ECOLI MALE_ECOLI] Involved in the high-affinity maltose membrane transport system MalEFGK. Initial receptor for the active transport of and chemotaxis toward maltooligosaccharides.[https://www.uniprot.org/uniprot/G3XD49_PSEAE G3XD49_PSEAE]
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Drug-resistant Pseudomonas aeruginosa (PA) poses an emerging threat to human health with urgent need for alternative therapeutic approaches. Here, we deciphered the B cell and antibody response to the virulence-associated type III secretion system (T3SS) in a cohort of patients chronically infected with PA. Single-cell analytics revealed a diverse B cell receptor repertoire directed against the T3SS needle-tip protein PcrV, enabling the production of monoclonal antibodies (mAbs) abrogating T3SS-mediated cytotoxicity. Mechanistic studies involving cryoelectron microscopy identified a surface-exposed C-terminal PcrV epitope as the target of highly neutralizing mAbs with broad activity against drug-resistant PA isolates. These anti-PcrV mAbs were as effective as treatment with conventional antibiotics in vivo. Our study reveals that chronically infected patients represent a source of neutralizing antibodies, which can be exploited as therapeutics against PA.

-	~~Authors: Yuan, B~~., Simonis, A., Marlovits, T.C.	+	Discovery of highly neutralizing human antibodies targeting Pseudomonas aeruginosa.,Simonis A, Kreer C, Albus A, Rox K, Yuan B, Holzmann D, Wilms JA, Zuber S, Kottege L, Winter S, Meyer M, Schmitt K, Gruell H, Theobald SJ, Hellmann AM, Meyer C, Ercanoglu MS, Cramer N, Munder A, Hallek M, Fatkenheuer G, Koch M, Seifert H, Rietschel E, Marlovits TC, van Koningsbruggen-Rietschel S, Klein F, Rybniker J Cell. 2023 Nov 9;186(23):5098-5113.e19. doi: 10.1016/j.cell.2023.10.002. Epub , 2023 Nov 1. PMID:37918395<ref>PMID:37918395</ref>

-	~~Description: Cryo-EM structure~~ of ~~PcrV~~/~~Fab(11~~-~~E5)~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased~~ Structures]]	+	</div>
-	[[Category: ~~Yuan, B~~]]	+	<div class="pdbe-citations 8crb" style="background-color:#fffaf0;"></div>
-	[[Category: Simonis, A]]	+	== References ==
-	[[Category: ~~Marlovits, T.C~~]]	+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Homo sapiens]]
		+	[[Category: Large Structures]]
		+	[[Category: Pseudomonas aeruginosa]]
		+	[[Category: Marlovits TC]]
		+	[[Category: Simonis A]]
		+	[[Category: Yuan B]]

OCA at 18:36, 12 April 2023

OCA — Wed, 12 Apr 2023 18:36:17 GMT

←Older revision		Revision as of 18:36, 12 April 2023
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 8crb is ON HOLD	+	The entry 8crb is ON HOLD until 2025-03-08

	Authors: Yuan, B., Simonis, A., Marlovits, T.C.		Authors: Yuan, B., Simonis, A., Marlovits, T.C.

OCA: Protected "8crb" [edit=sysop:move=sysop]

OCA — Wed, 15 Mar 2023 07:37:41 GMT

Protected "8crb" [edit=sysop:move=sysop]

←Older revision

Revision as of 07:37, 15 March 2023

OCA: New page: '''Unreleased structure''' The entry 8crb is ON HOLD Authors: Yuan, B., Simonis, A., Marlovits, T.C. Description: Cryo-EM structure of PcrV/Fab(11-E5) [[Category: Unreleased Structures...

OCA — Wed, 15 Mar 2023 07:37:40 GMT

New page: '''Unreleased structure''' The entry 8crb is ON HOLD Authors: Yuan, B., Simonis, A., Marlovits, T.C. Description: Cryo-EM structure of PcrV/Fab(11-E5) [[Category: Unreleased Structures...

New page

'''Unreleased structure'''

The entry 8crb is ON HOLD

Authors: Yuan, B., Simonis, A., Marlovits, T.C.

Description: Cryo-EM structure of PcrV/Fab(11-E5)
[[Category: Unreleased Structures]]
[[Category: Yuan, B]]
[[Category: Simonis, A]]
[[Category: Marlovits, T.C]]

8crb - Revision history

OCA at 07:27, 22 November 2023

OCA at 18:36, 12 April 2023

OCA: Protected "8crb" [edit=sysop:move=sysop]

OCA: New page: '''Unreleased structure''' The entry 8crb is ON HOLD Authors: Yuan, B., Simonis, A., Marlovits, T.C. Description: Cryo-EM structure of PcrV/Fab(11-E5) [[Category: Unreleased Structures...