OCA at 09:52, 15 March 2023

OCA — Wed, 15 Mar 2023 09:52:17 GMT

←Older revision		Revision as of 09:52, 15 March 2023
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 8d3t is ~~ON HOLD until Paper~~ Publication	+	==Crystal structure of GalS1 from Populus trichocarpas==
		+	<StructureSection load='8d3t' size='340' side='right'caption='[[8d3t]], [[Resolution\|resolution]] 2.37Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[8d3t]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Populus_trichocarpa Populus trichocarpa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8D3T OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8D3T FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BMA:BETA-D-MANNOSE'>BMA</scene>, <scene name='pdbligand=MAN:ALPHA-D-MANNOSE'>MAN</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8d3t FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8d3t OCA], [https://pdbe.org/8d3t PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8d3t RCSB], [https://www.ebi.ac.uk/pdbsum/8d3t PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8d3t ProSAT]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/A0A2K2AMS7_POPTR A0A2K2AMS7_POPTR]
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Rhamnogalacturonan I (RGI) is a structurally complex pectic polysaccharide with a backbone of alternating rhamnose and galacturonic acid residues substituted with arabinan and galactan side chains. Galactan synthase 1 (GalS1) transfers galactose and arabinose to either extend or cap the beta-1,4-galactan side chains of RGI, respectively. Here we report the structure of GalS1 from Populus trichocarpa, showing a modular protein consisting of an N-terminal domain that represents the founding member of a new family of carbohydrate-binding module, CBM95, and a C-terminal glycosyltransferase family 92 (GT92) catalytic domain that adopts a GT-A fold. GalS1 exists as a dimer in vitro, with stem domains interacting across the chains in a 'handshake' orientation that is essential for maintaining stability and activity. In addition to understanding the enzymatic mechanism of GalS1, we gained insight into the donor and acceptor substrate binding sites using deep evolutionary analysis, molecular simulations and biochemical studies. Combining all the results, a mechanism for GalS1 catalysis and a new model for pectic galactan side-chain addition are proposed.

-	~~Authors: Pereira~~, ~~J.H~~., Prabhakar, ~~P.K.~~, ~~Urbanowicz, B.~~R., Adams, P.D.	+	Structural and biochemical insight into a modular beta-1,4-galactan synthase in plants.,Prabhakar PK, Pereira JH, Taujale R, Shao W, Bharadwaj VS, Chapla D, Yang JY, Bomble YJ, Moremen KW, Kannan N, Hammel M, Adams PD, Scheller HV, Urbanowicz BR Nat Plants. 2023 Feb 27. doi: 10.1038/s41477-023-01358-4. PMID:36849618<ref>PMID:36849618</ref>

-	~~Description: Crystal structure~~ of ~~GalS1 from Populus trichocarpas~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased~~ Structures]]	+	</div>
-	[[Category: ~~Pereira, J.H~~]]	+	<div class="pdbe-citations 8d3t" style="background-color:#fffaf0;"></div>
-	[[Category: Adams~~, P.D~~]]	+	== References ==
-	[[Category: ~~Urbanowicz, B.R~~]]	+	<references/>
-	[[Category: Prabhakar~~, P.K~~]]	+	__TOC__
		+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Populus trichocarpa]]
		+	[[Category: Adams PD]]
		+	[[Category: Pereira JH]]
		+	[[Category: Prabhakar PK]]
		+	[[Category: Urbanowicz BR]]

OCA at 15:09, 6 July 2022

OCA — Wed, 06 Jul 2022 15:09:36 GMT

←Older revision		Revision as of 15:09, 6 July 2022
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 8d3t is ON HOLD	+	The entry 8d3t is ON HOLD until Paper Publication

	Authors: Pereira, J.H., Prabhakar, P.K., Urbanowicz, B.R., Adams, P.D.		Authors: Pereira, J.H., Prabhakar, P.K., Urbanowicz, B.R., Adams, P.D.
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	Description: Crystal structure of GalS1 from Populus trichocarpas		Description: Crystal structure of GalS1 from Populus trichocarpas
	[[Category: Unreleased Structures]]		[[Category: Unreleased Structures]]
-	[[Category: Adams, P.D]]
	[[Category: Pereira, J.H]]		[[Category: Pereira, J.H]]
		+	[[Category: Adams, P.D]]
	[[Category: Urbanowicz, B.R]]		[[Category: Urbanowicz, B.R]]
	[[Category: Prabhakar, P.K]]		[[Category: Prabhakar, P.K]]

OCA: Protected "8d3t" [edit=sysop:move=sysop]

OCA — Wed, 08 Jun 2022 04:35:48 GMT

Protected "8d3t" [edit=sysop:move=sysop]

←Older revision

Revision as of 04:35, 8 June 2022

OCA: New page: '''Unreleased structure''' The entry 8d3t is ON HOLD Authors: Pereira, J.H., Prabhakar, P.K., Urbanowicz, B.R., Adams, P.D. Description: Crystal structure of GalS1 from Populus trichoc...

OCA — Wed, 08 Jun 2022 04:35:47 GMT

New page: '''Unreleased structure''' The entry 8d3t is ON HOLD Authors: Pereira, J.H., Prabhakar, P.K., Urbanowicz, B.R., Adams, P.D. Description: Crystal structure of GalS1 from Populus trichoc...

New page

'''Unreleased structure'''

The entry 8d3t is ON HOLD

Authors: Pereira, J.H., Prabhakar, P.K., Urbanowicz, B.R., Adams, P.D.

Description: Crystal structure of GalS1 from Populus trichocarpas
[[Category: Unreleased Structures]]
[[Category: Adams, P.D]]
[[Category: Pereira, J.H]]
[[Category: Urbanowicz, B.R]]
[[Category: Prabhakar, P.K]]

8d3t - Revision history

OCA at 09:52, 15 March 2023

OCA at 15:09, 6 July 2022

OCA: Protected "8d3t" [edit=sysop:move=sysop]

OCA: New page: '''Unreleased structure''' The entry 8d3t is ON HOLD Authors: Pereira, J.H., Prabhakar, P.K., Urbanowicz, B.R., Adams, P.D. Description: Crystal structure of GalS1 from Populus trichoc...