OCA at 08:16, 14 June 2023

OCA — Wed, 14 Jun 2023 08:16:59 GMT

←Older revision		Revision as of 08:16, 14 June 2023
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-	'''Unreleased structure'''

-	~~The entry~~ 8d4c is ~~ON HOLD until Paper~~ Publication	+	==beta-Arf1 mediated dimeric assembly of AP-1, Arf1, Nef complex within lattice on MHC-I lipopeptide incorporated narrow membrane tubes==
		+	<StructureSection load='8d4c' size='340' side='right'caption='[[8d4c]], [[Resolution\|resolution]] 9.30Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[8d4c]] is a 18 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens], [https://en.wikipedia.org/wiki/Human_immunodeficiency_virus_1 Human immunodeficiency virus 1] and [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8D4C OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8D4C FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GTP:GUANOSINE-5-TRIPHOSPHATE'>GTP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8d4c FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8d4c OCA], [https://pdbe.org/8d4c PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8d4c RCSB], [https://www.ebi.ac.uk/pdbsum/8d4c PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8d4c ProSAT]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/ARF1_HUMAN ARF1_HUMAN] GTP-binding protein that functions as an allosteric activator of the cholera toxin catalytic subunit, an ADP-ribosyltransferase. Involved in protein trafficking among different compartments. Modulates vesicle budding and uncoating within the Golgi complex. Deactivation induces the redistribution of the entire Golgi complex to the endoplasmic reticulum, suggesting a crucial role in protein trafficking. In its GTP-bound form, its triggers the association with coat proteins with the Golgi membrane. The hydrolysis of ARF1-bound GTP, which is mediated by ARFGAPs proteins, is required for dissociation of coat proteins from Golgi membranes and vesicles.
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The adaptor protein (AP) complexes not only form the inner layer of clathrin coats but also have clathrin-independent roles in membrane traffic whose mechanisms are unknown. HIV-1 Nef hijacks AP-1 to sequester major histocompatibility complex class I (MHC-I), evading immune detection. We found that AP-1:Arf1:Nef:MHC-I forms a coat on tubulated membranes without clathrin and determined its structure. The coat assembles via Arf1 dimer interfaces. AP-1-positive tubules are enriched in cells upon clathrin knockdown. Nef localizes preferentially to AP-1 tubules in cells, explaining how Nef sequesters MHC-I. Coat contact residues are conserved across Arf isoforms and the Arf-dependent AP complexes AP-1, AP-3, and AP-4. Thus, AP complexes can self-assemble with Arf1 into tubular coats without clathrin or other scaffolding factors. The AP-1:Arf1 coat defines the structural basis of a broader class of tubulovesicular membrane coats as an intermediate in clathrin vesicle formation from internal membranes and as an MHC-I sequestration mechanism in HIV-1 infection.

-	~~Authors~~:	+	Self-assembly and structure of a clathrin-independent AP-1:Arf1 tubular membrane coat.,Hooy RM, Iwamoto Y, Tudorica DA, Ren X, Hurley JH Sci Adv. 2022 Oct 21;8(42):eadd3914. doi: 10.1126/sciadv.add3914. Epub 2022 Oct, 21. PMID:36269825<ref>PMID:36269825</ref>

-	~~Description~~:	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased~~ Structures]]	+	</div>
		+	<div class="pdbe-citations 8d4c" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Homo sapiens]]
		+	[[Category: Human immunodeficiency virus 1]]
		+	[[Category: Large Structures]]
		+	[[Category: Mus musculus]]
		+	[[Category: Hooy RM]]
		+	[[Category: Hurley JH]]

OCA at 17:04, 19 October 2022

OCA — Wed, 19 Oct 2022 17:04:28 GMT

←Older revision		Revision as of 17:04, 19 October 2022
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 8d4c is ON HOLD	+	The entry 8d4c is ON HOLD until Paper Publication

	Authors:		Authors:

OCA: Protected "8d4c" [edit=sysop:move=sysop]

OCA — Wed, 08 Jun 2022 04:36:40 GMT

Protected "8d4c" [edit=sysop:move=sysop]

←Older revision

Revision as of 04:36, 8 June 2022

OCA: New page: '''Unreleased structure''' The entry 8d4c is ON HOLD Authors: Description: Category: Unreleased Structures

OCA — Wed, 08 Jun 2022 04:36:39 GMT

New page: '''Unreleased structure''' The entry 8d4c is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 8d4c is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]

8d4c - Revision history

OCA at 08:16, 14 June 2023

OCA at 17:04, 19 October 2022

OCA: Protected "8d4c" [edit=sysop:move=sysop]

OCA: New page: '''Unreleased structure''' The entry 8d4c is ON HOLD Authors: Description: Category: Unreleased Structures