8d6x - Revision history

OCA at 07:50, 9 November 2022

OCA — Wed, 09 Nov 2022 07:50:15 GMT

←Older revision		Revision as of 07:50, 9 November 2022
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	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/A0A045JPX7_MYCTX A0A045JPX7_MYCTX]]	+	[https://www.uniprot.org/uniprot/A0A045JPX7_MYCTX A0A045JPX7_MYCTX]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==

OCA at 19:02, 7 September 2022

OCA — Wed, 07 Sep 2022 19:02:32 GMT

←Older revision		Revision as of 19:02, 7 September 2022
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	== Function ==		== Function ==
	[[https://www.uniprot.org/uniprot/A0A045JPX7_MYCTX A0A045JPX7_MYCTX]]		[[https://www.uniprot.org/uniprot/A0A045JPX7_MYCTX A0A045JPX7_MYCTX]]
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Mycobacterium tuberculosis possesses a Pup-proteasome system analogous to the eukaryotic ubiquitin-proteasome pathway. We have previously shown that the hexameric mycobacterial proteasome ATPase (Mpa) recruits pupylated protein substrates via interactions between amino-terminal coiled-coils in Mpa monomers and the degradation tag Pup. However, it is unclear how Mpa rings interact with a proteasome due to the presence of a carboxyl-terminal beta-grasp domain unique to Mpa homologues that makes the interaction highly unstable. Here, we describe newly identified critical interactions between Mpa and 20S core proteasomes. Interestingly, the Mpa C-terminal GQYL motif binds the 20S core particle activation pocket differently than the same motif of the ATP-independent proteasome accessory factor PafE. We further found that the beta-hairpin of the Mpa beta-grasp domain interacts variably with the H0 helix on top of the 20S core particle via a series of ionic and hydrogen-bond interactions. Individually mutating several involved residues reduced Mpa-mediated protein degradation both in vitro and in vivo. IMPORTANCE The Pup-proteasome system in Mycobacterium tuberculosis is critical for this species to cause lethal infections in mice. Investigating the molecular mechanism of how the Mpa ATPase recruits and unfolds pupylated substrates to the 20S proteasomal core particle for degradation will be essential to fully understand how degradation is regulated, and the structural information we report may be useful for the development of new tuberculosis chemotherapies.
		+
		+	The beta-Grasp Domain of Proteasomal ATPase Mpa Makes Critical Contacts with the Mycobacterium tuberculosis 20S Core Particle to Facilitate Degradation.,Xiao X, Feng X, Yoo JH, Kovach A, Darwin KH, Li H mSphere. 2022 Aug 22:e0027422. doi: 10.1128/msphere.00274-22. PMID:35993699<ref>PMID:35993699</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 8d6x" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>

OCA at 16:51, 7 September 2022

OCA — Wed, 07 Sep 2022 16:51:52 GMT

←Older revision		Revision as of 16:51, 7 September 2022
Line 1:		Line 1:

-	====	+	==Structure of the Mycobacterium tuberculosis 20S proteasome bound to the ATP-bound Mpa ATPase==
-	<StructureSection load='8d6x' size='340' side='right'caption='[[8d6x]]' scene=''>	+	<StructureSection load='8d6x' size='340' side='right'caption='[[8d6x]], [[Resolution\|resolution]] 3.20Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id= OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol= FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[8d6x]] is a 41 chain structure with sequence from [https://en.wikipedia.org/wiki/Mycobacterium_tuberculosis Mycobacterium tuberculosis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8D6X OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8D6X FirstGlance]. <br>
	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8d6x FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8d6x OCA], [https://pdbe.org/8d6x PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8d6x RCSB], [https://www.ebi.ac.uk/pdbsum/8d6x PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8d6x ProSAT]</span></td></tr>		</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8d6x FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8d6x OCA], [https://pdbe.org/8d6x PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8d6x RCSB], [https://www.ebi.ac.uk/pdbsum/8d6x PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8d6x ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[[https://www.uniprot.org/uniprot/A0A045JPX7_MYCTX A0A045JPX7_MYCTX]]
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Z-disk~~]]	+	[[Category: Mycobacterium tuberculosis]]
		+	[[Category: Li H]]
		+	[[Category: Xiao X]]

OCA at 04:56, 3 August 2022

OCA — Wed, 03 Aug 2022 04:56:10 GMT

←Older revision		Revision as of 04:56, 3 August 2022
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-	'''Unreleased structure'''

-	~~The entry~~ 8d6x ~~is ON HOLD~~	+	====
-		+	<StructureSection load='8d6x' size='340' side='right'caption='[[8d6x]]' scene=''>
-	~~Authors~~: ~~Xiao, X~~.~~, Li, H~~.	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id= OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol= FirstGlance]. <br>
-	~~Description~~: ~~Structure of the Mycobacterium tuberculosis 20S proteasome bound to the ATP~~-~~bound Mpa ATPase~~	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8d6x FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8d6x OCA], [https://pdbe.org/8d6x PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8d6x RCSB], [https://www.ebi.ac.uk/pdbsum/8d6x PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8d6x ProSAT]</span></td></tr>
-	[[~~Category~~: ~~Unreleased Structures~~]]	+	</table>
-	[[Category: ~~Li, H~~]]	+	__TOC__
-	[[Category: ~~Xiao, X~~]]	+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Z-disk]]

OCA at 15:10, 6 July 2022

OCA — Wed, 06 Jul 2022 15:10:41 GMT

←Older revision		Revision as of 15:10, 6 July 2022
Line 5:		Line 5:
	Authors: Xiao, X., Li, H.		Authors: Xiao, X., Li, H.

-	Description: ~~The beta grasp domain~~ of ~~proteasomal ATPase Mpa makes critical contacts with~~ the Mycobacterium tuberculosis 20S proteasome to ~~facilitate degradation~~	+	Description: Structure of the Mycobacterium tuberculosis 20S proteasome bound to the ATP-bound Mpa ATPase
	[[Category: Unreleased Structures]]		[[Category: Unreleased Structures]]
	[[Category: Li, H]]		[[Category: Li, H]]
	[[Category: Xiao, X]]		[[Category: Xiao, X]]

OCA: Protected "8d6x" [edit=sysop:move=sysop]

OCA — Wed, 15 Jun 2022 04:13:11 GMT

Protected "8d6x" [edit=sysop:move=sysop]

←Older revision

Revision as of 04:13, 15 June 2022

OCA: New page: '''Unreleased structure''' The entry 8d6x is ON HOLD Authors: Xiao, X., Li, H. Description: The beta grasp domain of proteasomal ATPase Mpa makes critical contacts with the Mycobacteri...

OCA — Wed, 15 Jun 2022 04:13:10 GMT

New page: '''Unreleased structure''' The entry 8d6x is ON HOLD Authors: Xiao, X., Li, H. Description: The beta grasp domain of proteasomal ATPase Mpa makes critical contacts with the Mycobacteri...

New page

'''Unreleased structure'''

The entry 8d6x is ON HOLD

Authors: Xiao, X., Li, H.

Description: The beta grasp domain of proteasomal ATPase Mpa makes critical contacts with the Mycobacterium tuberculosis 20S proteasome to facilitate degradation
[[Category: Unreleased Structures]]
[[Category: Li, H]]
[[Category: Xiao, X]]