OCA at 07:41, 11 January 2023

OCA — Wed, 11 Jan 2023 07:41:25 GMT

←Older revision		Revision as of 07:41, 11 January 2023
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-	'''Unreleased structure'''

-	~~The entry~~ 8d9k is ~~ON HOLD~~	+	==CryoEM structure of human METTL1-WDR4 in complex with Lys-tRNA==
		+	<StructureSection load='8d9k' size='340' side='right'caption='[[8d9k]], [[Resolution\|resolution]] 3.72Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[8d9k]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8D9K OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8D9K FirstGlance]. <br>
		+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8d9k FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8d9k OCA], [https://pdbe.org/8d9k PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8d9k RCSB], [https://www.ebi.ac.uk/pdbsum/8d9k PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8d9k ProSAT]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/TRMB_HUMAN TRMB_HUMAN] Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA.<ref>PMID:12403464</ref>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Specific, regulated modification of RNAs is important for proper gene expression(1,2). tRNAs are rich with various chemical modifications that affect their stability and function(3,4). 7-Methylguanosine (m(7)G) at tRNA position 46 is a conserved modification that modulates steady-state tRNA levels to affect cell growth(5,6). The METTL1-WDR4 complex generates m(7)G46 in humans, and dysregulation of METTL1-WDR4 has been linked to brain malformation and multiple cancers(7-22). Here we show how METTL1 and WDR4 cooperate to recognize RNA substrates and catalyse methylation. A crystal structure of METTL1-WDR4 and cryo-electron microscopy structures of METTL1-WDR4-tRNA show that the composite protein surface recognizes the tRNA elbow through shape complementarity. The cryo-electron microscopy structures of METTL1-WDR4-tRNA with S-adenosylmethionine or S-adenosylhomocysteine along with METTL1 crystal structures provide additional insights into the catalytic mechanism by revealing the active site in multiple states. The METTL1 N terminus couples cofactor binding with conformational changes in the tRNA, the catalytic loop and the WDR4 C terminus, acting as the switch to activate m(7)G methylation. Thus, our structural models explain how post-translational modifications of the METTL1 N terminus can regulate methylation. Together, our work elucidates the core and regulatory mechanisms underlying m(7)G modification by METTL1, providing the framework to understand its contribution to biology and disease.

-	~~Authors~~:	+	Structures and mechanisms of tRNA methylation by METTL1-WDR4.,Ruiz-Arroyo VM, Raj R, Babu K, Onolbaatar O, Roberts PH, Nam Y Nature. 2023 Jan 4. doi: 10.1038/s41586-022-05565-5. PMID:36599982<ref>PMID:36599982</ref>

-	~~Description~~:	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased~~ Structures]]	+	</div>
		+	<div class="pdbe-citations 8d9k" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Homo sapiens]]
		+	[[Category: Large Structures]]
		+	[[Category: Nam Y]]
		+	[[Category: Ruiz-Arroyo VM]]

OCA at 15:29, 7 September 2022

OCA — Wed, 07 Sep 2022 15:29:04 GMT

←Older revision		Revision as of 15:29, 7 September 2022
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 8d9k is ON HOLD ~~until Paper Publication~~	+	The entry 8d9k is ON HOLD

	Authors:		Authors:

OCA at 03:59, 10 August 2022

OCA — Wed, 10 Aug 2022 03:59:41 GMT

←Older revision		Revision as of 03:59, 10 August 2022
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 8d9k is ON HOLD	+	The entry 8d9k is ON HOLD until Paper Publication

	Authors:		Authors:

OCA: Protected "8d9k" [edit=sysop:move=sysop]

OCA — Wed, 22 Jun 2022 07:26:53 GMT

Protected "8d9k" [edit=sysop:move=sysop]

←Older revision

Revision as of 07:26, 22 June 2022

OCA: New page: '''Unreleased structure''' The entry 8d9k is ON HOLD Authors: Description: Category: Unreleased Structures

OCA — Wed, 22 Jun 2022 07:26:52 GMT

New page: '''Unreleased structure''' The entry 8d9k is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 8d9k is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]

8d9k - Revision history

OCA at 07:41, 11 January 2023

OCA at 15:29, 7 September 2022

OCA at 03:59, 10 August 2022

OCA: Protected "8d9k" [edit=sysop:move=sysop]

OCA: New page: '''Unreleased structure''' The entry 8d9k is ON HOLD Authors: Description: Category: Unreleased Structures