OCA at 06:36, 25 January 2023

OCA — Wed, 25 Jan 2023 06:36:08 GMT

←Older revision		Revision as of 06:36, 25 January 2023
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-	'''Unreleased structure'''

-	~~The entry~~ 8dbw is ~~ON HOLD until Paper~~ Publication	+	==E. coli ATP synthase imaged in 10mM MgATP State3 "down" Fo classified==
		+	<StructureSection load='8dbw' size='340' side='right'caption='[[8dbw]], [[Resolution\|resolution]] 4.10Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[8dbw]] is a 22 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8DBW OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8DBW FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene>, <scene name='pdbligand=ATP:ADENOSINE-5-TRIPHOSPHATE'>ATP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8dbw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8dbw OCA], [https://pdbe.org/8dbw PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8dbw RCSB], [https://www.ebi.ac.uk/pdbsum/8dbw PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8dbw ProSAT]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/A0A7U9G3U3_ECOLX A0A7U9G3U3_ECOLX] Produces ATP from ADP in the presence of a proton gradient across the membrane. The alpha chain is a regulatory subunit.[ARBA:ARBA00003784][HAMAP-Rule:MF_01346]
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	F(1)F(o) ATP synthase functions as a biological generator and makes a major contribution to cellular energy production. Proton flow generates rotation in the F(o) motor that is transferred to the F(1) motor to catalyze ATP production, with flexible F(1)/F(o) coupling required for efficient catalysis. F(1)F(o) ATP synthase can also operate in reverse, hydrolyzing ATP and pumping protons, and in bacteria this function can be regulated by an inhibitory epsilon subunit. Here we present cryo-EM data showing E. coli F(1)F(o) ATP synthase in different rotational and inhibited sub-states, observed following incubation with 10 mM MgATP. Our structures demonstrate how structural transitions within the inhibitory epsilon subunit induce torsional movement in the central stalk, thereby enabling its rotation within the F(omicron) motor. This highlights the importance of the central rotor for flexible coupling of the F(1) and F(o) motors and provides further insight into the regulatory mechanism mediated by subunit epsilon.

-	~~Authors: Sobti~~, M., ~~Stewart~~, A.G.	+	Changes within the central stalk of E. coli F(1)F(o) ATP synthase observed after addition of ATP.,Sobti M, Zeng YC, Walshe JL, Brown SHJ, Ishmukhametov R, Stewart AG Commun Biol. 2023 Jan 11;6(1):26. doi: 10.1038/s42003-023-04414-z. PMID:36631659<ref>PMID:36631659</ref>

-	~~Description: E~~. ~~coli ATP synthase imaged in 10mM MgATP State3~~ ""~~down~~"" ~~Fo classified~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased~~ Structures]]	+	</div>
-	[[Category: Sobti, M]]	+	<div class="pdbe-citations 8dbw" style="background-color:#fffaf0;"></div>
-	[[Category: Stewart~~, A.G~~]]	+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Escherichia coli]]
		+	[[Category: Large Structures]]
		+	[[Category: Sobti M]]
		+	[[Category: Stewart AG]]

OCA at 04:25, 20 July 2022

OCA — Wed, 20 Jul 2022 04:25:46 GMT

←Older revision		Revision as of 04:25, 20 July 2022
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 8dbw is ON HOLD	+	The entry 8dbw is ON HOLD until Paper Publication

	Authors: Sobti, M., Stewart, A.G.		Authors: Sobti, M., Stewart, A.G.

OCA: Protected "8dbw" [edit=sysop:move=sysop]

OCA — Wed, 22 Jun 2022 07:30:13 GMT

Protected "8dbw" [edit=sysop:move=sysop]

←Older revision

Revision as of 07:30, 22 June 2022

OCA: New page: '''Unreleased structure''' The entry 8dbw is ON HOLD Authors: Sobti, M., Stewart, A.G. Description: E. coli ATP synthase imaged in 10mM MgATP State3 ""down"" Fo classified [[Category: ...

OCA — Wed, 22 Jun 2022 07:30:12 GMT

New page: '''Unreleased structure''' The entry 8dbw is ON HOLD Authors: Sobti, M., Stewart, A.G. Description: E. coli ATP synthase imaged in 10mM MgATP State3 ""down"" Fo classified [[Category: ...

New page

'''Unreleased structure'''

The entry 8dbw is ON HOLD

Authors: Sobti, M., Stewart, A.G.

Description: E. coli ATP synthase imaged in 10mM MgATP State3 ""down"" Fo classified
[[Category: Unreleased Structures]]
[[Category: Sobti, M]]
[[Category: Stewart, A.G]]

8dbw - Revision history

OCA at 06:36, 25 January 2023

OCA at 04:25, 20 July 2022

OCA: Protected "8dbw" [edit=sysop:move=sysop]

OCA: New page: '''Unreleased structure''' The entry 8dbw is ON HOLD Authors: Sobti, M., Stewart, A.G. Description: E. coli ATP synthase imaged in 10mM MgATP State3 ""down"" Fo classified [[Category: ...