OCA at 06:46, 8 February 2023

OCA — Wed, 08 Feb 2023 06:46:43 GMT

←Older revision		Revision as of 06:46, 8 February 2023
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-	'''Unreleased structure'''

-	~~The entry~~ 8dm9 is ~~ON HOLD until Paper~~ Publication	+	==Cryo-EM structure of SARS-CoV-2 Omicron BA.1 spike protein in complex with mouse ACE2==
		+	<StructureSection load='8dm9' size='340' side='right'caption='[[8dm9]], [[Resolution\|resolution]] 2.56Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[8dm9]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus] and [https://en.wikipedia.org/wiki/Severe_acute_respiratory_syndrome_coronavirus_2 Severe acute respiratory syndrome coronavirus 2]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8DM9 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8DM9 FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8dm9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8dm9 OCA], [https://pdbe.org/8dm9 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8dm9 RCSB], [https://www.ebi.ac.uk/pdbsum/8dm9 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8dm9 ProSAT]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/ACE2_MOUSE ACE2_MOUSE] Essential counter-regulatory carboxypeptidase of the renin-angiotensin hormone system that is a critical regulator of blood volume, systemic vascular resistance, and thus cardiovascular homeostasis. Converts angiotensin I to angiotensin 1-9, a nine-amino acid peptide with anti-hypertrophic effects in cardiomyocytes, and angiotensin II to angiotensin 1-7, which then acts as a beneficial vasodilator and anti-proliferation agent, counterbalancing the actions of the vasoconstrictor angiotensin II. Also removes the C-terminal residue from three other vasoactive peptides, neurotensin, kinetensin, and des-Arg bradykinin, but is not active on bradykinin. Also cleaves other biological peptides, such as apelins, casomorphins and dynorphin A (By similarity). By cleavage of angiotensin II, may be an important regulator of heart function (PubMed:12075344, PubMed:12967627). By cleavage of angiotensin II, may also have a protective role in acute lung injury (PubMed:16001071). Plays an important role in amino acid transport by acting as binding partner of amino acid transporter SLC6A19, regulating its trafficking on the cell surface and its activity (PubMed:19185582, PubMed:18424768, PubMed:22677001).[UniProtKB:Q9BYF1]<ref>PMID:12075344</ref> <ref>PMID:12967627</ref> <ref>PMID:16001071</ref> <ref>PMID:18424768</ref> <ref>PMID:19185582</ref> <ref>PMID:22677001</ref>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The BA.2 sub-lineage of the Omicron (B.1.1.529) severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) variant rapidly supplanted the original BA.1 sub-lineage in early 2022. Both lineages threatened the efficacy of vaccine-elicited antibodies and acquired increased binding to several mammalian ACE2 receptors. Cryoelectron microscopy (cryo-EM) analysis of the BA.2 spike (S) glycoprotein in complex with mouse ACE2 (mACE2) identifies BA.1- and BA.2-mutated residues Q493R, N501Y, and Y505H as complementing non-conserved residues between human and mouse ACE2, rationalizing the enhanced S protein-mACE2 interaction for Omicron variants. Cryo-EM structures of the BA.2 S-human ACE2 complex and of the extensively mutated BA.2 amino-terminal domain (NTD) reveal a dramatic reorganization of the highly antigenic N1 loop into a beta-strand, providing an explanation for decreased binding of the BA.2 S protein to antibodies isolated from BA.1-convalescent patients. Our analysis reveals structural mechanisms underlying the antigenic drift in the rapidly evolving Omicron variant landscape.

-	~~Authors: Zhu, X~~., Saville~~, J.W.~~, Mannar, D., Berezuk~~, A.M.~~, Cholak, S., Tuttle~~, K.S.~~, Vahdatihassani, F., Subramaniam, S.	+	Structural analysis of receptor engagement and antigenic drift within the BA.2 spike protein.,Saville JW, Mannar D, Zhu X, Berezuk AM, Cholak S, Tuttle KS, Vahdatihassani F, Subramaniam S Cell Rep. 2023 Jan 4;42(1):111964. doi: 10.1016/j.celrep.2022.111964. PMID:36640338<ref>PMID:36640338</ref>

-	~~Description: Cryo-EM structure~~ of ~~SARS~~-~~CoV~~-~~2 Omicron BA.1 spike protein in complex with mouse ACE2~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased~~ Structures]]	+	</div>
-	[[Category: ~~Subramaniam, S~~]]	+	<div class="pdbe-citations 8dm9" style="background-color:#fffaf0;"></div>
-	[[Category: ~~Zhu, X~~]]	+	== References ==
-	[[Category: ~~Tuttle, K.~~S]]	+	<references/>
-	[[Category: Mannar, D]]	+	__TOC__
-	[[Category: Saville~~, J.W~~]]	+	</StructureSection>
-	[[Category: ~~Vahdatihassani, F~~]]	+	[[Category: Large Structures]]
-	[[Category: ~~Cholak, S~~]]	+	[[Category: Mus musculus]]
-	[[Category: ~~Berezuk, A.M~~]]	+	[[Category: Severe acute respiratory syndrome coronavirus 2]]
		+	[[Category: Berezuk AM]]
		+	[[Category: Cholak S]]
		+	[[Category: Mannar D]]
		+	[[Category: Saville JW]]
		+	[[Category: Subramaniam S]]
		+	[[Category: Tuttle KS]]
		+	[[Category: Vahdatihassani F]]
		+	[[Category: Zhu X]]

OCA at 04:01, 18 August 2022

OCA — Thu, 18 Aug 2022 04:01:38 GMT

←Older revision		Revision as of 04:01, 18 August 2022
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 8dm9 is ON HOLD	+	The entry 8dm9 is ON HOLD until Paper Publication

	Authors: Zhu, X., Saville, J.W., Mannar, D., Berezuk, A.M., Cholak, S., Tuttle, K.S., Vahdatihassani, F., Subramaniam, S.		Authors: Zhu, X., Saville, J.W., Mannar, D., Berezuk, A.M., Cholak, S., Tuttle, K.S., Vahdatihassani, F., Subramaniam, S.

OCA: Protected "8dm9" [edit=sysop:move=sysop]

OCA — Wed, 13 Jul 2022 04:20:43 GMT

Protected "8dm9" [edit=sysop:move=sysop]

←Older revision

Revision as of 04:20, 13 July 2022

OCA: New page: '''Unreleased structure''' The entry 8dm9 is ON HOLD Authors: Zhu, X., Saville, J.W., Mannar, D., Berezuk, A.M., Cholak, S., Tuttle, K.S., Vahdatihassani, F., Subramaniam, S. Descripti...

OCA — Wed, 13 Jul 2022 04:20:42 GMT

New page: '''Unreleased structure''' The entry 8dm9 is ON HOLD Authors: Zhu, X., Saville, J.W., Mannar, D., Berezuk, A.M., Cholak, S., Tuttle, K.S., Vahdatihassani, F., Subramaniam, S. Descripti...

New page

'''Unreleased structure'''

The entry 8dm9 is ON HOLD

Authors: Zhu, X., Saville, J.W., Mannar, D., Berezuk, A.M., Cholak, S., Tuttle, K.S., Vahdatihassani, F., Subramaniam, S.

Description: Cryo-EM structure of SARS-CoV-2 Omicron BA.1 spike protein in complex with mouse ACE2
[[Category: Unreleased Structures]]
[[Category: Subramaniam, S]]
[[Category: Zhu, X]]
[[Category: Tuttle, K.S]]
[[Category: Mannar, D]]
[[Category: Saville, J.W]]
[[Category: Vahdatihassani, F]]
[[Category: Cholak, S]]
[[Category: Berezuk, A.M]]

8dm9 - Revision history

OCA at 06:46, 8 February 2023

OCA at 04:01, 18 August 2022

OCA: Protected "8dm9" [edit=sysop:move=sysop]

OCA: New page: '''Unreleased structure''' The entry 8dm9 is ON HOLD Authors: Zhu, X., Saville, J.W., Mannar, D., Berezuk, A.M., Cholak, S., Tuttle, K.S., Vahdatihassani, F., Subramaniam, S. Descripti...