OCA at 07:23, 22 March 2023

OCA — Wed, 22 Mar 2023 07:23:35 GMT

←Older revision		Revision as of 07:23, 22 March 2023
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-	'''Unreleased structure'''

-	~~The entry~~ 8dna is ~~ON HOLD until Paper~~ Publication	+	==Acidipropionibacterium acidipropionici encapsulin in a closed state at pH 3.0==
		+	<StructureSection load='8dna' size='340' side='right'caption='[[8dna]], [[Resolution\|resolution]] 2.77Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[8dna]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Acidipropionibacterium_acidipropionici_ATCC_4875 Acidipropionibacterium acidipropionici ATCC 4875]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8DNA OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8DNA FirstGlance]. <br>
		+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8dna FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8dna OCA], [https://pdbe.org/8dna PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8dna RCSB], [https://www.ebi.ac.uk/pdbsum/8dna PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8dna ProSAT]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/K7RV67_ACIA4 K7RV67_ACIA4]
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Encapsulins are microbial protein nanocages capable of efficient self-assembly and cargo enzyme encapsulation. Due to their favorable properties, including high thermostability, protease resistance, and robust heterologous expression, encapsulins have become popular bioengineering tools for applications in medicine, catalysis, and nanotechnology. Resistance against physicochemical extremes like high temperature and low pH is a highly desirable feature for many biotechnological applications. However, no systematic search for acid-stable encapsulins has been carried out, while the influence of pH on encapsulin shells has so far not been thoroughly explored. Here, we report on a newly identified encapsulin nanocage from the acid-tolerant bacterium Acidipropionibacterium acidipropionici. Using transmission electron microscopy, dynamic light scattering, and proteolytic assays, we demonstrate its extreme acid tolerance and resilience against proteases. We structurally characterize the novel nanocage using cryo-electron microscopy, revealing a dynamic five-fold pore that displays distinct "closed" and "open" states at neutral pH but only a singular "closed" state under strongly acidic conditions. Further, the "open" state exhibits the largest pore in an encapsulin shell reported to date. Non-native protein encapsulation capabilities are demonstrated, and the influence of external pH on internalized cargo is explored. Our results expand the biotechnological application range of encapsulin nanocages toward potential uses under strongly acidic conditions and highlight pH-responsive encapsulin pore dynamics.

-	~~Authors~~:	+	Exploring the Extreme Acid Tolerance of a Dynamic Protein Nanocage.,Jones JA, Andreas MP, Giessen TW Biomacromolecules. 2023 Mar 13;24(3):1388-1399. doi: 10.1021/acs.biomac.2c01424. , Epub 2023 Feb 16. PMID:36796007<ref>PMID:36796007</ref>

-	~~Description~~:	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased~~ Structures]]	+	</div>
		+	<div class="pdbe-citations 8dna" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Acidipropionibacterium acidipropionici ATCC 4875]]
		+	[[Category: Large Structures]]
		+	[[Category: Andreas MP]]
		+	[[Category: Giessen TW]]
		+	[[Category: Jones JA]]

OCA at 04:01, 10 August 2022

OCA — Wed, 10 Aug 2022 04:01:50 GMT

←Older revision		Revision as of 04:01, 10 August 2022
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 8dna is ON HOLD	+	The entry 8dna is ON HOLD until Paper Publication

	Authors:		Authors:

OCA: Protected "8dna" [edit=sysop:move=sysop]

OCA — Wed, 20 Jul 2022 04:31:30 GMT

Protected "8dna" [edit=sysop:move=sysop]

←Older revision

Revision as of 04:31, 20 July 2022

OCA: New page: '''Unreleased structure''' The entry 8dna is ON HOLD Authors: Description: Category: Unreleased Structures

OCA — Wed, 20 Jul 2022 04:31:29 GMT

New page: '''Unreleased structure''' The entry 8dna is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 8dna is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]

8dna - Revision history

OCA at 07:23, 22 March 2023

OCA at 04:01, 10 August 2022

OCA: Protected "8dna" [edit=sysop:move=sysop]

OCA: New page: '''Unreleased structure''' The entry 8dna is ON HOLD Authors: Description: Category: Unreleased Structures