8dol - Revision history

OCA at 07:25, 3 April 2024

OCA — Wed, 03 Apr 2024 07:25:45 GMT

←Older revision		Revision as of 07:25, 3 April 2024
Line 4:		Line 4:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[8dol]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Helicobacter_pylori_26695 Helicobacter pylori 26695]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8DOL OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8DOL FirstGlance]. <br>		<table><tr><td colspan='2'>[[8dol]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Helicobacter_pylori_26695 Helicobacter pylori 26695]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8DOL OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8DOL FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.8Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8dol FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8dol OCA], [https://pdbe.org/8dol PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8dol RCSB], [https://www.ebi.ac.uk/pdbsum/8dol PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8dol ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8dol FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8dol OCA], [https://pdbe.org/8dol PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8dol RCSB], [https://www.ebi.ac.uk/pdbsum/8dol PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8dol ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
	[https://www.uniprot.org/uniprot/O25260_HELPY O25260_HELPY]		[https://www.uniprot.org/uniprot/O25260_HELPY O25260_HELPY]
-	<div style="background-color:#fffaf0;">
-	== Publication Abstract from PubMed ==
-	The transport of the CagA effector into gastric epithelial cells by the Cag Type IV secretion system (Cag T4SS) of Helicobacter pylori (H. pylori) is critical for pathogenesis. CagA is recruited to Cag T4SS by the Cagbeta ATPase. CagZ, a unique protein in H. pylori, regulates Cagbeta-mediated CagA transport, but the underlying mechanisms remain unclear. Here we report the crystal structure of the cytosolic region of Cagbeta, showing a typical ring-like hexameric assembly. The central channel of the ring is narrow, suggesting that CagA must unfold for transport through the channel. Our structure of CagZ in complex with the all-alpha domain (AAD) of Cagbeta shows that CagZ adopts an overall U-shape and tightly embraces Cagbeta. This binding mode of CagZ is incompatible with the formation of the Cagbeta hexamer essential for the ATPase activity. CagZ therefore inhibits Cagbeta by trapping it in the monomeric state. Based on these findings, we propose a refined model for the transport of CagA by Cagbeta.
-
-	Mechanism of regulation of the Helicobacter pylori Cagbeta ATPase by CagZ.,Wu X, Zhao Y, Zhang H, Yang W, Yang J, Sun L, Jiang M, Wang Q, Wang Q, Ye X, Zhang X, Wu Y Nat Commun. 2023 Jan 30;14(1):479. doi: 10.1038/s41467-023-36218-4. PMID:36717564<ref>PMID:36717564</ref>
-
-	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	</div>
-	<div class="pdbe-citations 8dol" style="background-color:#fffaf0;"></div>
-	== References ==
-	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>

OCA at 10:27, 15 February 2023

OCA — Wed, 15 Feb 2023 10:27:59 GMT

←Older revision		Revision as of 10:27, 15 February 2023
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 8dol is ~~ON HOLD until Paper~~ Publication	+	==Mechanism of regulation of the Helicobacter pylori Cagbeta ATPase by CagZ==
		+	<StructureSection load='8dol' size='340' side='right'caption='[[8dol]], [[Resolution\|resolution]] 2.80Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[8dol]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Helicobacter_pylori_26695 Helicobacter pylori 26695]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8DOL OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8DOL FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8dol FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8dol OCA], [https://pdbe.org/8dol PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8dol RCSB], [https://www.ebi.ac.uk/pdbsum/8dol PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8dol ProSAT]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/O25260_HELPY O25260_HELPY]
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The transport of the CagA effector into gastric epithelial cells by the Cag Type IV secretion system (Cag T4SS) of Helicobacter pylori (H. pylori) is critical for pathogenesis. CagA is recruited to Cag T4SS by the Cagbeta ATPase. CagZ, a unique protein in H. pylori, regulates Cagbeta-mediated CagA transport, but the underlying mechanisms remain unclear. Here we report the crystal structure of the cytosolic region of Cagbeta, showing a typical ring-like hexameric assembly. The central channel of the ring is narrow, suggesting that CagA must unfold for transport through the channel. Our structure of CagZ in complex with the all-alpha domain (AAD) of Cagbeta shows that CagZ adopts an overall U-shape and tightly embraces Cagbeta. This binding mode of CagZ is incompatible with the formation of the Cagbeta hexamer essential for the ATPase activity. CagZ therefore inhibits Cagbeta by trapping it in the monomeric state. Based on these findings, we propose a refined model for the transport of CagA by Cagbeta.

-	~~Authors:~~ Wu, X., Zhao, Y., Yang, W., Sun, L~~., Ye, X.~~, Jiang, M., Wang, Q., Wang, Q., ~~Zhang~~, X., Wu, Y.	+	Mechanism of regulation of the Helicobacter pylori Cagbeta ATPase by CagZ.,Wu X, Zhao Y, Zhang H, Yang W, Yang J, Sun L, Jiang M, Wang Q, Wang Q, Ye X, Zhang X, Wu Y Nat Commun. 2023 Jan 30;14(1):479. doi: 10.1038/s41467-023-36218-4. PMID:36717564<ref>PMID:36717564</ref>

-	~~Description: Mechanism of regulation~~ of the Helicobacter pylori ~~Cagbeta ATPase by CagZ~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased~~ Structures]]	+	</div>
-	[[Category: ~~Wu, X~~]]	+	<div class="pdbe-citations 8dol" style="background-color:#fffaf0;"></div>
-	[[Category: ~~Wang, Q~~]]	+	== References ==
-	[[Category: ~~Wu, Y~~]]	+	<references/>
-	[[Category: ~~Jiang, M~~]]	+	__TOC__
-	[[Category: ~~Zhao,~~ Y]]	+	</StructureSection>
-	[[Category: Yang, W]]	+	[[Category: Helicobacter pylori 26695]]
-	[[Category: ~~Zhang,~~ X]]	+	[[Category: Large Structures]]
-	[[Category: ~~Ye,~~ X]]	+	[[Category: Jiang M]]
-	[[Category: ~~Sun, L~~]]	+	[[Category: Sun L]]
		+	[[Category: Wang Q]]
		+	[[Category: Wu X]]
		+	[[Category: Wu Y]]
		+	[[Category: Yang W]]
		+	[[Category: Ye X]]
		+	[[Category: Zhang X]]
		+	[[Category: Zhao Y]]

OCA at 18:04, 16 November 2022

OCA — Wed, 16 Nov 2022 18:04:33 GMT

←Older revision		Revision as of 18:04, 16 November 2022
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 8dol is ON HOLD	+	The entry 8dol is ON HOLD until Paper Publication

	Authors: Wu, X., Zhao, Y., Yang, W., Sun, L., Ye, X., Jiang, M., Wang, Q., Wang, Q., Zhang, X., Wu, Y.		Authors: Wu, X., Zhao, Y., Yang, W., Sun, L., Ye, X., Jiang, M., Wang, Q., Wang, Q., Zhang, X., Wu, Y.
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	[[Category: Unreleased Structures]]		[[Category: Unreleased Structures]]
	[[Category: Wu, X]]		[[Category: Wu, X]]
		+	[[Category: Wang, Q]]
		+	[[Category: Wu, Y]]
	[[Category: Jiang, M]]		[[Category: Jiang, M]]
	[[Category: Zhao, Y]]		[[Category: Zhao, Y]]
		+	[[Category: Yang, W]]
	[[Category: Zhang, X]]		[[Category: Zhang, X]]
	[[Category: Ye, X]]		[[Category: Ye, X]]
-	[[Category: Wang, Q]]
-	[[Category: Wu, Y]]
-	[[Category: Yang, W]]
	[[Category: Sun, L]]		[[Category: Sun, L]]

OCA: Protected "8dol" [edit=sysop:move=sysop]

OCA — Wed, 20 Jul 2022 04:36:13 GMT

Protected "8dol" [edit=sysop:move=sysop]

←Older revision

Revision as of 04:36, 20 July 2022

OCA: New page: '''Unreleased structure''' The entry 8dol is ON HOLD Authors: Wu, X., Zhao, Y., Yang, W., Sun, L., Ye, X., Jiang, M., Wang, Q., Wang, Q., Zhang, X., Wu, Y. Description: Mechanism of re...

OCA — Wed, 20 Jul 2022 04:36:12 GMT

New page: '''Unreleased structure''' The entry 8dol is ON HOLD Authors: Wu, X., Zhao, Y., Yang, W., Sun, L., Ye, X., Jiang, M., Wang, Q., Wang, Q., Zhang, X., Wu, Y. Description: Mechanism of re...

New page

'''Unreleased structure'''

The entry 8dol is ON HOLD

Authors: Wu, X., Zhao, Y., Yang, W., Sun, L., Ye, X., Jiang, M., Wang, Q., Wang, Q., Zhang, X., Wu, Y.

Description: Mechanism of regulation of the Helicobacter pylori Cagbeta ATPase by CagZ
[[Category: Unreleased Structures]]
[[Category: Wu, X]]
[[Category: Jiang, M]]
[[Category: Zhao, Y]]
[[Category: Zhang, X]]
[[Category: Ye, X]]
[[Category: Wang, Q]]
[[Category: Wu, Y]]
[[Category: Yang, W]]
[[Category: Sun, L]]