OCA at 08:20, 7 December 2022

OCA — Wed, 07 Dec 2022 08:20:50 GMT

←Older revision		Revision as of 08:20, 7 December 2022
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-	'''Unreleased structure'''

-	~~The entry~~ 8dq1 is ~~ON HOLD~~	+	==Quorum-sensing receptor RhlR bound to PqsE==
		+	<StructureSection load='8dq1' size='340' side='right'caption='[[8dq1]], [[Resolution\|resolution]] 4.10Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[8dq1]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_aeruginosa Pseudomonas aeruginosa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8DQ1 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8DQ1 FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=K5G:4-(3-bromophenoxy)-N-[(3S)-2-oxothiolan-3-yl]butanamide'>K5G</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8dq1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8dq1 OCA], [https://pdbe.org/8dq1 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8dq1 RCSB], [https://www.ebi.ac.uk/pdbsum/8dq1 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8dq1 ProSAT]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/A9JPX4_PSEAI A9JPX4_PSEAI]
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Pseudomonas aeruginosa is an opportunistic pathogen that is responsible for thousands of deaths every year in the United States. P. aeruginosa virulence factor production is mediated by quorum sensing, a mechanism of bacterial cell-cell communication that relies on the production and detection of signal molecules called autoinducers. In P. aeruginosa, the transcription factor receptor RhlR is activated by a RhlI-synthesized autoinducer. We recently showed that RhlR-dependent transcription is enhanced by a physical interaction with the enzyme PqsE via increased affinity of RhlR for promoter DNA. However, the molecular basis for complex formation and how complex formation enhanced RhlR transcriptional activity remained unclear. Here, we report the structure of ligand-bound RhlR in complex with PqsE. Additionally, we determined the structure of the complex bound with DNA, revealing the mechanism by which RhlR-mediated transcription is enhanced by PqsE, thereby establishing the molecular basis for RhlR-dependent virulence factor production in P. aeruginosa.

-	~~Authors~~:	+	Structure of the RhlR-PqsE complex from Pseudomonas aeruginosa reveals mechanistic insights into quorum-sensing gene regulation.,Feathers JR, Richael EK, Simanek KA, Fromme JC, Paczkowski JE Structure. 2022 Dec 1;30(12):1626-1636.e4. doi: 10.1016/j.str.2022.10.008. Epub , 2022 Nov 14. PMID:36379213<ref>PMID:36379213</ref>

-	~~Description~~:	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased~~ Structures]]	+	</div>
		+	<div class="pdbe-citations 8dq1" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Pseudomonas aeruginosa]]
		+	[[Category: Feathers JR]]
		+	[[Category: Fromme JC]]
		+	[[Category: Paczkowski JE]]

OCA: Protected "8dq1" [edit=sysop:move=sysop]

OCA — Wed, 27 Jul 2022 16:45:51 GMT

Protected "8dq1" [edit=sysop:move=sysop]

←Older revision

Revision as of 16:45, 27 July 2022

OCA: New page: '''Unreleased structure''' The entry 8dq1 is ON HOLD Authors: Description: Category: Unreleased Structures

OCA — Wed, 27 Jul 2022 16:45:50 GMT

New page: '''Unreleased structure''' The entry 8dq1 is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 8dq1 is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]

8dq1 - Revision history

OCA at 08:20, 7 December 2022

OCA: Protected "8dq1" [edit=sysop:move=sysop]

OCA: New page: '''Unreleased structure''' The entry 8dq1 is ON HOLD Authors: Description: Category: Unreleased Structures