OCA at 21:16, 28 June 2023

OCA — Wed, 28 Jun 2023 21:16:59 GMT

←Older revision		Revision as of 21:16, 28 June 2023
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 8ds6 is ~~ON HOLD until Paper~~ Publication	+	==Structure of the PEAK3 pseudokinase homodimer==
		+	<StructureSection load='8ds6' size='340' side='right'caption='[[8ds6]], [[Resolution\|resolution]] 4.90Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[8ds6]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8DS6 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8DS6 FirstGlance]. <br>
		+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution\|Resolution]] 4.9Å</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8ds6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8ds6 OCA], [https://pdbe.org/8ds6 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8ds6 RCSB], [https://www.ebi.ac.uk/pdbsum/8ds6 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8ds6 ProSAT]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/PEAK3_HUMAN PEAK3_HUMAN] Probable catalytically inactive kinase (Probable). Interacts with CRK-II and antagonizes CRK-II-signaling. Prevents the formation of CRK-II-dependent membrane ruffling and lamellipodia-like extensions (PubMed:31311869).<ref>PMID:31311869</ref> <ref>PMID:31311869</ref>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	PEAK pseudokinases are molecular scaffolds which dimerize to regulate cell migration, morphology, and proliferation, as well as cancer progression. The mechanistic role dimerization plays in PEAK scaffolding remains unclear, as there are no structures of PEAKs in complex with their interactors. Here, we report the cryo-EM structure of dimeric PEAK3 in complex with an endogenous 14-3-3 heterodimer. Our structure reveals an asymmetric binding mode between PEAK3 and 14-3-3 stabilized by one pseudokinase domain and the SHED domain of the PEAK3 dimer. The binding interface contains a canonical phosphosite-dependent primary interaction and a unique secondary interaction not observed in previous structures of 14-3-3/client complexes. Additionally, we show that PKD regulates PEAK3/14-3-3 binding, which when prevented leads to PEAK3 nuclear enrichment and distinct protein-protein interactions. Altogether, our data demonstrate that PEAK3 dimerization forms an unusual secondary interface for 14-3-3 binding, facilitating 14-3-3 regulation of PEAK3 localization and interactome diversity.

-	~~Authors~~:	+	Structural insights into regulation of the PEAK3 pseudokinase scaffold by 14-3-3.,Torosyan H, Paul MD, Forget A, Lo M, Diwanji D, Pawlowski K, Krogan NJ, Jura N, Verba KA Nat Commun. 2023 Jun 19;14(1):3543. doi: 10.1038/s41467-023-38864-0. PMID:37336883<ref>PMID:37336883</ref>

-	~~Description~~:	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased~~ Structures]]	+	</div>
		+	<div class="pdbe-citations 8ds6" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Homo sapiens]]
		+	[[Category: Large Structures]]
		+	[[Category: Jura N]]
		+	[[Category: Paul M]]
		+	[[Category: Torosyan H]]
		+	[[Category: Verba KA]]

OCA at 03:54, 3 August 2022

OCA — Wed, 03 Aug 2022 03:54:44 GMT

←Older revision		Revision as of 03:54, 3 August 2022
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 8ds6 is ON HOLD	+	The entry 8ds6 is ON HOLD until Paper Publication

	Authors:		Authors:

OCA: Protected "8ds6" [edit=sysop:move=sysop]

OCA — Wed, 27 Jul 2022 16:49:27 GMT

Protected "8ds6" [edit=sysop:move=sysop]

←Older revision

Revision as of 16:49, 27 July 2022

OCA: New page: '''Unreleased structure''' The entry 8ds6 is ON HOLD Authors: Description: Category: Unreleased Structures

OCA — Wed, 27 Jul 2022 16:49:26 GMT

New page: '''Unreleased structure''' The entry 8ds6 is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 8ds6 is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]

8ds6 - Revision history

OCA at 21:16, 28 June 2023

OCA at 03:54, 3 August 2022

OCA: Protected "8ds6" [edit=sysop:move=sysop]

OCA: New page: '''Unreleased structure''' The entry 8ds6 is ON HOLD Authors: Description: Category: Unreleased Structures