8dvq - Revision history

OCA at 10:45, 22 May 2024

OCA — Wed, 22 May 2024 10:45:56 GMT

←Older revision		Revision as of 10:45, 22 May 2024
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	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[8dvq]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Enterococcus Enterococcus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8DVQ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8DVQ FirstGlance]. <br>		<table><tr><td colspan='2'>[[8dvq]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Enterococcus Enterococcus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8DVQ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8DVQ FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CD:CADMIUM+ION'>CD</scene></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.19Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CD:CADMIUM+ION'>CD</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8dvq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8dvq OCA], [https://pdbe.org/8dvq PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8dvq RCSB], [https://www.ebi.ac.uk/pdbsum/8dvq PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8dvq ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8dvq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8dvq OCA], [https://pdbe.org/8dvq PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8dvq RCSB], [https://www.ebi.ac.uk/pdbsum/8dvq PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8dvq ProSAT]</span></td></tr>
	</table>		</table>
Line 13:		Line 14:
	The VanRS two-component system regulates the resistance phenotype of vancomycin-resistant enterococci. VanS is a sensor histidine kinase that responds to the presence of vancomycin by autophosphorylating and subsequently transferring the phosphoryl group to the response regulator, VanR. The phosphotransfer activates VanR as a transcription factor, which initiates the expression of resistance genes. Structural information about VanS proteins has remained elusive, hindering the molecular-level understanding of their function. Here, we present X-ray crystal structures for the catalytic and ATP-binding (CA) domains of two VanS proteins, derived from vancomycin-resistant enterococci types A and C. Both proteins adopt the canonical Bergerat fold that has been observed for CA domains of other prokaryotic histidine kinases. We attempted to determine structures for the nucleotide-bound forms of both proteins; however, despite repeated efforts, these forms could not be crystallized, prompting us to measure the proteins' binding affinities for ATP. Unexpectedly, both CA domains displayed low affinities for the nucleotide, with K(D) values in the low millimolar range. Since these K(D) values are comparable to intracellular ATP concentrations, this weak substrate binding could reflect a way of regulating expression of the resistance phenotype.		The VanRS two-component system regulates the resistance phenotype of vancomycin-resistant enterococci. VanS is a sensor histidine kinase that responds to the presence of vancomycin by autophosphorylating and subsequently transferring the phosphoryl group to the response regulator, VanR. The phosphotransfer activates VanR as a transcription factor, which initiates the expression of resistance genes. Structural information about VanS proteins has remained elusive, hindering the molecular-level understanding of their function. Here, we present X-ray crystal structures for the catalytic and ATP-binding (CA) domains of two VanS proteins, derived from vancomycin-resistant enterococci types A and C. Both proteins adopt the canonical Bergerat fold that has been observed for CA domains of other prokaryotic histidine kinases. We attempted to determine structures for the nucleotide-bound forms of both proteins; however, despite repeated efforts, these forms could not be crystallized, prompting us to measure the proteins' binding affinities for ATP. Unexpectedly, both CA domains displayed low affinities for the nucleotide, with K(D) values in the low millimolar range. Since these K(D) values are comparable to intracellular ATP concentrations, this weak substrate binding could reflect a way of regulating expression of the resistance phenotype.

-	Structure of VanS from vancomycin-resistant enterococci: A sensor kinase with weak ATP binding.,Grasty KC, Guzik C, D'Lauro EJ, Padrick SB, Beld J, Loll PJ J Biol Chem. 2023 ~~Feb 9~~;299(3):103001. doi: 10.1016/j.jbc.2023.103001. PMID:36764524<ref>PMID:36764524</ref>	+	Structure of VanS from vancomycin-resistant enterococci: A sensor kinase with weak ATP binding.,Grasty KC, Guzik C, D'Lauro EJ, Padrick SB, Beld J, Loll PJ J Biol Chem. 2023 Mar;299(3):103001. doi: 10.1016/j.jbc.2023.103001. Epub 2023 , Feb 9. PMID:36764524<ref>PMID:36764524</ref>

	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>

OCA at 07:24, 22 March 2023

OCA — Wed, 22 Mar 2023 07:24:09 GMT

←Older revision		Revision as of 07:24, 22 March 2023
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-	'''Unreleased structure'''

-	~~The entry~~ 8dvq is ~~ON HOLD until Paper~~ Publication	+	==CA domain of VanSA histidine kinase==
		+	<StructureSection load='8dvq' size='340' side='right'caption='[[8dvq]], [[Resolution\|resolution]] 2.19Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[8dvq]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Enterococcus Enterococcus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8DVQ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8DVQ FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CD:CADMIUM+ION'>CD</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8dvq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8dvq OCA], [https://pdbe.org/8dvq PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8dvq RCSB], [https://www.ebi.ac.uk/pdbsum/8dvq PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8dvq ProSAT]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/VANS_ENTFC VANS_ENTFC] Member of the two-component regulatory system VanS/VanR. Activates the transcription of vanH, vanA and vanX in response to vancomycin which results in vancomycin resistance. VanS activates VanR by phosphorylation.
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The VanRS two-component system regulates the resistance phenotype of vancomycin-resistant enterococci. VanS is a sensor histidine kinase that responds to the presence of vancomycin by autophosphorylating and subsequently transferring the phosphoryl group to the response regulator, VanR. The phosphotransfer activates VanR as a transcription factor, which initiates the expression of resistance genes. Structural information about VanS proteins has remained elusive, hindering the molecular-level understanding of their function. Here, we present X-ray crystal structures for the catalytic and ATP-binding (CA) domains of two VanS proteins, derived from vancomycin-resistant enterococci types A and C. Both proteins adopt the canonical Bergerat fold that has been observed for CA domains of other prokaryotic histidine kinases. We attempted to determine structures for the nucleotide-bound forms of both proteins; however, despite repeated efforts, these forms could not be crystallized, prompting us to measure the proteins' binding affinities for ATP. Unexpectedly, both CA domains displayed low affinities for the nucleotide, with K(D) values in the low millimolar range. Since these K(D) values are comparable to intracellular ATP concentrations, this weak substrate binding could reflect a way of regulating expression of the resistance phenotype.

-	~~Authors~~: ~~Loll, P~~.J.	+	Structure of VanS from vancomycin-resistant enterococci: A sensor kinase with weak ATP binding.,Grasty KC, Guzik C, D'Lauro EJ, Padrick SB, Beld J, Loll PJ J Biol Chem. 2023 Feb 9;299(3):103001. doi: 10.1016/j.jbc.2023.103001. PMID:36764524<ref>PMID:36764524</ref>

-	~~Description: CA domain~~ of ~~VanSA histidine kinase~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased~~ Structures]]	+	</div>
-	[[Category: Loll~~, P.J~~]]	+	<div class="pdbe-citations 8dvq" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Enterococcus]]
		+	[[Category: Large Structures]]
		+	[[Category: Loll PJ]]

OCA: Protected "8dvq" [edit=sysop:move=sysop]

OCA — Wed, 10 Aug 2022 04:03:41 GMT

Protected "8dvq" [edit=sysop:move=sysop]

←Older revision

Revision as of 04:03, 10 August 2022

OCA: New page: '''Unreleased structure''' The entry 8dvq is ON HOLD until Paper Publication Authors: Loll, P.J. Description: CA domain of VanSA histidine kinase Category: Unreleased Structures [[...

OCA — Wed, 10 Aug 2022 04:03:41 GMT

New page: '''Unreleased structure''' The entry 8dvq is ON HOLD until Paper Publication Authors: Loll, P.J. Description: CA domain of VanSA histidine kinase Category: Unreleased Structures [[...

New page

'''Unreleased structure'''

The entry 8dvq is ON HOLD until Paper Publication

Authors: Loll, P.J.

Description: CA domain of VanSA histidine kinase
[[Category: Unreleased Structures]]
[[Category: Loll, P.J]]