8e0u - Revision history

OCA at 17:32, 18 October 2023

OCA — Wed, 18 Oct 2023 17:32:46 GMT

←Older revision		Revision as of 17:32, 18 October 2023
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	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[8e0u]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_K-12 Escherichia coli K-12]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8E0U OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8E0U FirstGlance]. <br>		<table><tr><td colspan='2'>[[8e0u]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_K-12 Escherichia coli K-12]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8E0U OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8E0U FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.42Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8e0u FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8e0u OCA], [https://pdbe.org/8e0u PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8e0u RCSB], [https://www.ebi.ac.uk/pdbsum/8e0u PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8e0u ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8e0u FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8e0u OCA], [https://pdbe.org/8e0u PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8e0u RCSB], [https://www.ebi.ac.uk/pdbsum/8e0u PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8e0u ProSAT]</span></td></tr>
	</table>		</table>
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	</div>		</div>
	<div class="pdbe-citations 8e0u" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 8e0u" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[DAHP synthase 3D structures\|DAHP synthase 3D structures]]
	== References ==		== References ==
	<references/>		<references/>

OCA at 07:36, 3 November 2022

OCA — Thu, 03 Nov 2022 07:36:57 GMT

←Older revision		Revision as of 07:36, 3 November 2022
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 8e0u is ~~ON HOLD until Paper~~ Publication	+	==DAHP (3-deoxy-D-arabinoheptulosonate-7-phosphate) Synthase complexed with Sulfate in unbound:(bound)2:other conformations==
		+	<StructureSection load='8e0u' size='340' side='right'caption='[[8e0u]], [[Resolution\|resolution]] 2.42Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[8e0u]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_K-12 Escherichia coli K-12]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8E0U OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8E0U FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8e0u FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8e0u OCA], [https://pdbe.org/8e0u PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8e0u RCSB], [https://www.ebi.ac.uk/pdbsum/8e0u PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8e0u ProSAT]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/AROG_ECOLI AROG_ECOLI] Stereospecific condensation of phosphoenolpyruvate (PEP) and D-erythrose-4-phosphate (E4P) giving rise to 3-deoxy-D-arabino-heptulosonate-7-phosphate (DAHP).
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	alpha-Carboxyketose synthases, including 3-deoxy-d-arabinoheptulosonate 7-phosphate synthase (DAHPS), are long-standing targets for inhibition. They are challenging targets to create tight-binding inhibitors against, and inhibitors often display half-of-sites binding and partial inhibition. Half-of-sites inhibition demonstrates the existence of inter-subunit communication in DAHPS. We used X-ray crystallography and spatially resolved hydrogen-deuterium exchange (HDX) to reveal the structural and dynamic bases for inter-subunit communication in Escherichia coli DAHPS(Phe), the isozyme that is feedback-inhibited by phenylalanine. Crystal structures of this homotetrameric (dimer-of-dimers) enzyme are invariant over 91% of its sequence. Three variable loops make up 8% of the sequence and are all involved in inter-subunit contacts across the tight-dimer interface. The structures have pseudo-twofold symmetry indicative of inter-subunit communication across the loose-dimer interface, with the diagonal subunits B and C always having the same conformation as each other, while subunits A and D are variable. Spatially resolved HDX reveals contrasting responses to ligand binding, which, in turn, affect binding of the second substrate, erythrose-4-phosphate (E4P). The N-terminal peptide, M1-E12, and the active site loop that binds E4P, F95-K105, are key parts of the communication network. Inter-subunit communication appears to have a catalytic role in all alpha-carboxyketose synthase families and a regulatory role in some members.

-	~~Authors: Berti~~, P~~.J.~~, Junop, M.S., ~~Grainger, R~~.	+	Role of Half-of-Sites Reactivity and Inter-Subunit Communications in DAHP Synthase Catalysis and Regulation.,Balachandran N, Grainger RA, Rob T, Liuni P, Wilson DJ, Junop MS, Berti PJ Biochemistry. 2022 Oct 18;61(20):2229-2240. doi: 10.1021/acs.biochem.2c00465., Epub 2022 Oct 5. PMID:36197914<ref>PMID:36197914</ref>

-	~~Description: DAHP (3-deoxy-D-arabinoheptulosonate~~-7-~~phosphate) Synthase complexed with Sulfate in unbound~~:~~(bound)2~~:~~other conformations~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased~~ Structures]]	+	</div>
-	[[Category: ~~Grainger, R~~]]	+	<div class="pdbe-citations 8e0u" style="background-color:#fffaf0;"></div>
-	[[Category: ~~Berti, P.J~~]]	+	== References ==
-	[[Category: Junop~~, M.S~~]]	+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Escherichia coli K-12]]
		+	[[Category: Large Structures]]
		+	[[Category: Berti PJ]]
		+	[[Category: Grainger R]]
		+	[[Category: Junop MS]]

OCA at 05:45, 14 September 2022

OCA — Wed, 14 Sep 2022 05:45:05 GMT

←Older revision		Revision as of 05:45, 14 September 2022
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 8e0u is ON HOLD	+	The entry 8e0u is ON HOLD until Paper Publication

	Authors: Berti, P.J., Junop, M.S., Grainger, R.		Authors: Berti, P.J., Junop, M.S., Grainger, R.
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	Description: DAHP (3-deoxy-D-arabinoheptulosonate-7-phosphate) Synthase complexed with Sulfate in unbound:(bound)2:other conformations		Description: DAHP (3-deoxy-D-arabinoheptulosonate-7-phosphate) Synthase complexed with Sulfate in unbound:(bound)2:other conformations
	[[Category: Unreleased Structures]]		[[Category: Unreleased Structures]]
-	[[Category: Berti, P.J]]
	[[Category: Grainger, R]]		[[Category: Grainger, R]]
		+	[[Category: Berti, P.J]]
	[[Category: Junop, M.S]]		[[Category: Junop, M.S]]

OCA: Protected "8e0u" [edit=sysop:move=sysop]

OCA — Thu, 18 Aug 2022 04:11:36 GMT

Protected "8e0u" [edit=sysop:move=sysop]

←Older revision

Revision as of 04:11, 18 August 2022

OCA: New page: '''Unreleased structure''' The entry 8e0u is ON HOLD Authors: Berti, P.J., Junop, M.S., Grainger, R. Description: DAHP (3-deoxy-D-arabinoheptulosonate-7-phosphate) Synthase complexed w...

OCA — Thu, 18 Aug 2022 04:11:34 GMT

New page: '''Unreleased structure''' The entry 8e0u is ON HOLD Authors: Berti, P.J., Junop, M.S., Grainger, R. Description: DAHP (3-deoxy-D-arabinoheptulosonate-7-phosphate) Synthase complexed w...

New page

'''Unreleased structure'''

The entry 8e0u is ON HOLD

Authors: Berti, P.J., Junop, M.S., Grainger, R.

Description: DAHP (3-deoxy-D-arabinoheptulosonate-7-phosphate) Synthase complexed with Sulfate in unbound:(bound)2:other conformations
[[Category: Unreleased Structures]]
[[Category: Berti, P.J]]
[[Category: Grainger, R]]
[[Category: Junop, M.S]]