8e7x - Revision history

OCA at 10:19, 25 October 2023

OCA — Wed, 25 Oct 2023 10:19:11 GMT

←Older revision		Revision as of 10:19, 25 October 2023
Line 4:		Line 4:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[8e7x]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Cereibacter_sphaeroides Cereibacter sphaeroides]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8E7X OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8E7X FirstGlance]. <br>		<table><tr><td colspan='2'>[[8e7x]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Cereibacter_sphaeroides Cereibacter sphaeroides]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8E7X OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8E7X FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene>, <scene name='pdbligand=OLC:(2R)-2,3-DIHYDROXYPROPYL+(9Z)-OCTADEC-9-ENOATE'>OLC</scene></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.1Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene>, <scene name='pdbligand=OLC:(2R)-2,3-DIHYDROXYPROPYL+(9Z)-OCTADEC-9-ENOATE'>OLC</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8e7x FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8e7x OCA], [https://pdbe.org/8e7x PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8e7x RCSB], [https://www.ebi.ac.uk/pdbsum/8e7x PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8e7x ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8e7x FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8e7x OCA], [https://pdbe.org/8e7x PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8e7x RCSB], [https://www.ebi.ac.uk/pdbsum/8e7x PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8e7x ProSAT]</span></td></tr>
	</table>		</table>

OCA at 06:51, 29 March 2023

OCA — Wed, 29 Mar 2023 06:51:15 GMT

←Older revision		Revision as of 06:51, 29 March 2023
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-	'''Unreleased structure'''

-	~~The entry~~ 8e7x is ~~ON HOLD~~	+	==RsTSPO A138F with one Heme bound==
		+	<StructureSection load='8e7x' size='340' side='right'caption='[[8e7x]], [[Resolution\|resolution]] 2.10Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[8e7x]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Cereibacter_sphaeroides Cereibacter sphaeroides]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8E7X OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8E7X FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene>, <scene name='pdbligand=OLC:(2R)-2,3-DIHYDROXYPROPYL+(9Z)-OCTADEC-9-ENOATE'>OLC</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8e7x FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8e7x OCA], [https://pdbe.org/8e7x PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8e7x RCSB], [https://www.ebi.ac.uk/pdbsum/8e7x PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8e7x ProSAT]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The ancient protein TSPO (translocator protein 18kD) is found in all kingdoms and was originally identified as a binding site of benzodiazepine drugs. Its physiological function remains unclear, although porphyrins are conserved ligands. Several crystal structures of bacterial TSPO and nuclear magnetic resonance structures of a mouse form have revealed monomer and dimer configurations, but there have been no reports of structures with a physiological ligand. Here, we present the first X-ray structures of Rhodobacter sphaeroides TSPO with a physiological ligand bound. Two different variants (substituting threonine for alanine at position 139 (A139T) and phenylalanine for alanine at position 138 (A138F)) yielded well-diffracting crystals giving structures of both apo- and heme-containing forms. Both variants have wild-type micromolar affinity for heme and protoporphyrin IX, but A139T has very low ability to accelerate the breakdown of porphyrin in the presence of light and oxygen. The binding of heme to one protomer of the dimer of either mutant induces a more rigid structure, both in the heme-binding protomer and the protomer without heme bound, demonstrating an allosteric response. Ensemble refinement of the X-ray data reveals distinct regions of altered flexibility in response to single heme binding to the dimer. The A139T variant shows a more rigid structure overall, which may relate to extra hydrogen bonding of waters captured in the heme crevice. As TSPO has been suggested to have a role in heme delivery from mitochondria to the cytoplasm, the new structures provide potential clues regarding the structural basis of such activity.

-	~~Authors~~:	+	New TSPO Crystal Structures of Mutant and Heme-Bound Forms with Altered Flexibility, Ligand Binding, and Porphyrin Degradation Activity.,Liu J, Hiser C, Li F, Hall R, Garavito RM, Ferguson-Miller S Biochemistry. 2023 Mar 22. doi: 10.1021/acs.biochem.2c00612. PMID:36947867<ref>PMID:36947867</ref>

-	~~Description~~:	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased~~ Structures]]	+	</div>
		+	<div class="pdbe-citations 8e7x" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Cereibacter sphaeroides]]
		+	[[Category: Large Structures]]
		+	[[Category: Ferguson-Miller S]]
		+	[[Category: Garavito R]]
		+	[[Category: Hiser C]]
		+	[[Category: Li F]]
		+	[[Category: Liu J]]

OCA: Protected "8e7x" [edit=sysop:move=sysop]

OCA — Wed, 31 Aug 2022 05:39:01 GMT

Protected "8e7x" [edit=sysop:move=sysop]

←Older revision

Revision as of 05:39, 31 August 2022

OCA: New page: '''Unreleased structure''' The entry 8e7x is ON HOLD Authors: Description: Category: Unreleased Structures

OCA — Wed, 31 Aug 2022 05:38:59 GMT

New page: '''Unreleased structure''' The entry 8e7x is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 8e7x is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]