8ebi - Revision history

OCA at 18:14, 20 September 2023

OCA — Wed, 20 Sep 2023 18:14:26 GMT

←Older revision		Revision as of 18:14, 20 September 2023
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	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[8ebi]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Mycobacterium_tuberculosis Mycobacterium tuberculosis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8EBI OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8EBI FirstGlance]. <br>		<table><tr><td colspan='2'>[[8ebi]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Mycobacterium_tuberculosis Mycobacterium tuberculosis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8EBI OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8EBI FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene>, <scene name='pdbligand=TSL:TRANS-ENAMINE+INTERMEDIATE+OF+SULBACTAM'>TSL</scene></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.7Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene>, <scene name='pdbligand=TSL:TRANS-ENAMINE+INTERMEDIATE+OF+SULBACTAM'>TSL</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8ebi FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8ebi OCA], [https://pdbe.org/8ebi PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8ebi RCSB], [https://www.ebi.ac.uk/pdbsum/8ebi PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8ebi ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8ebi FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8ebi OCA], [https://pdbe.org/8ebi PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8ebi RCSB], [https://www.ebi.ac.uk/pdbsum/8ebi PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8ebi ProSAT]</span></td></tr>
	</table>		</table>

OCA at 07:35, 8 March 2023

OCA — Wed, 08 Mar 2023 07:35:39 GMT

←Older revision		Revision as of 07:35, 8 March 2023
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-	'''Unreleased structure'''

-	~~The entry~~ 8ebi is ~~ON HOLD until Paper~~ Publication	+	==XFEL structure of beta lactamase microcrystals mixed with sulbactam solution for 15ms==
		+	<StructureSection load='8ebi' size='340' side='right'caption='[[8ebi]], [[Resolution\|resolution]] 2.70Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[8ebi]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Mycobacterium_tuberculosis Mycobacterium tuberculosis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8EBI OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8EBI FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene>, <scene name='pdbligand=TSL:TRANS-ENAMINE+INTERMEDIATE+OF+SULBACTAM'>TSL</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8ebi FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8ebi OCA], [https://pdbe.org/8ebi PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8ebi RCSB], [https://www.ebi.ac.uk/pdbsum/8ebi PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8ebi ProSAT]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/A0A655AHQ9_MYCTX A0A655AHQ9_MYCTX]
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	For decades, researchers have been determined to elucidate essential enzymatic functions on the atomic lengths scale by tracing atomic positions in real time. Our work builds on new possibilities unleashed by mix-and-inject serial crystallography (MISC) (1-5) at X-ray free electron laser facilities. In this approach, enzymatic reactions are triggered by mixing substrate or ligand solutions with enzyme microcrystals (6) . Here, we report in atomic detail and with millisecond time-resolution how the Mycobacterium tuberculosis enzyme BlaC is inhibited by sulbactam (SUB). Our results reveal ligand binding heterogeneity, ligand gating (7-9) , cooperativity, induced fit (10,11) and conformational selection (11-13) all from the same set of MISC data, detailing how SUB approaches the catalytic clefts and binds to the enzyme non-covalently before reacting to a trans- enamine. This was made possible in part by the application of the singular value decomposition (14) to the MISC data using a newly developed program that remains functional even if unit cell parameters change during the reaction.

-	~~Authors~~:	+	Heterogeneity in the M. tuberculosis beta-Lactamase Inhibition by Sulbactam.,Schmidt M, Malla TN, Zielinski K, Aldama L, Bajt S, Feliz D, Hayes B, Hunter M, Kupitz C, Lisova S, Knoska J, Martin-Garcia J, Mariani V, Pandey S, Poudyal I, Sierra R, Tolstikova A, Yefanov O, Yoon CH, Ourmazd A, Fromme P, Schwander P, Barty A, Chapman H, Stojkovic E, Batyuk A, Boutet S, Phillips G, Pollack L Res Sq. 2023 Jan 10:rs.3.rs-2334665. doi: 10.21203/rs.3.rs-2334665/v1. Preprint. PMID:36712138<ref>PMID:36712138</ref>

-	~~Description~~:	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased~~ Structures]]	+	</div>
		+	<div class="pdbe-citations 8ebi" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Mycobacterium tuberculosis]]
		+	[[Category: Malla TN]]
		+	[[Category: Schmidt M]]

OCA at 03:57, 26 October 2022

OCA — Wed, 26 Oct 2022 03:57:36 GMT

←Older revision		Revision as of 03:57, 26 October 2022
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 8ebi is ON HOLD	+	The entry 8ebi is ON HOLD until Paper Publication

	Authors:		Authors:

OCA: Protected "8ebi" [edit=sysop:move=sysop]

OCA — Wed, 07 Sep 2022 15:37:32 GMT

Protected "8ebi" [edit=sysop:move=sysop]

←Older revision

Revision as of 15:37, 7 September 2022

OCA: New page: '''Unreleased structure''' The entry 8ebi is ON HOLD Authors: Description: Category: Unreleased Structures

OCA — Wed, 07 Sep 2022 15:37:32 GMT

New page: '''Unreleased structure''' The entry 8ebi is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 8ebi is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]