OCA at 05:53, 23 August 2023

OCA — Wed, 23 Aug 2023 05:53:31 GMT

←Older revision		Revision as of 05:53, 23 August 2023
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 8ehw is ~~ON HOLD until Paper~~ Publication	+	==cryo-EM structure of TMEM63A in nanodisc==
		+	<StructureSection load='8ehw' size='340' side='right'caption='[[8ehw]], [[Resolution\|resolution]] 3.80Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[8ehw]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8EHW OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8EHW FirstGlance]. <br>
		+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution\|Resolution]] 3.8Å</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8ehw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8ehw OCA], [https://pdbe.org/8ehw PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8ehw RCSB], [https://www.ebi.ac.uk/pdbsum/8ehw PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8ehw ProSAT]</span></td></tr>
		+	</table>
		+	== Disease ==
		+	[https://www.uniprot.org/uniprot/CSCL1_HUMAN CSCL1_HUMAN] The disease is caused by variants affecting the gene represented in this entry.
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/CSCL1_HUMAN CSCL1_HUMAN] Acts as an osmosensitive calcium-permeable cation channel (PubMed:30382938, PubMed:31587869). Mechanosensitive ion channel that converts mechanical stimuli into a flow of ion (PubMed:30382938, PubMed:31587869).[UniProtKB:Q91YT8]<ref>PMID:30382938</ref> <ref>PMID:31587869</ref>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	OSCA/TMEM63s form mechanically activated (MA) ion channels in plants and animals, respectively. OSCAs and related TMEM16s and transmembrane channel-like (TMC) proteins form homodimers with two pores. Here, we uncover an unanticipated monomeric configuration of TMEM63 proteins. Structures of TMEM63A and TMEM63B (referred to as TMEM63s) revealed a single highly restricted pore. Functional analyses demonstrated that TMEM63s are bona fide mechanosensitive ion channels, characterized by small conductance and high thresholds. TMEM63s possess evolutionary variations in the intracellular linker IL2, which mediates dimerization in OSCAs. Replacement of OSCA1.2 IL2 with TMEM63A IL2 or mutations to key variable residues resulted in monomeric OSCA1.2 and MA currents with significantly higher thresholds. Structural analyses revealed substantial conformational differences in the mechano-sensing domain IL2 and gating helix TM6 between TMEM63s and OSCA1.2. Our studies reveal that mechanosensitivity in OSCA/TMEM63 channels is affected by oligomerization and suggest gating mechanisms that may be shared by OSCA/TMEM63, TMEM16, and TMC channels.

-	~~Authors: Zheng~~, W., Fu, ~~T.M.~~, ~~Holt~~, J.R.	+	TMEM63 proteins function as monomeric high-threshold mechanosensitive ion channels.,Zheng W, Rawson S, Shen Z, Tamilselvan E, Smith HE, Halford J, Shen C, Murthy SE, Ulbrich MH, Sotomayor M, Fu TM, Holt JR Neuron. 2023 Jul 28:S0896-6273(23)00512-3. doi: 10.1016/j.neuron.2023.07.006. PMID:37543036<ref>PMID:37543036</ref>

-	~~Description: cryo-EM structure~~ of ~~TMEM63A in nanodisc~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased~~ Structures]]	+	</div>
-	[[Category: Fu~~, T.M~~]]	+	<div class="pdbe-citations 8ehw" style="background-color:#fffaf0;"></div>
-	[[Category: Holt~~, J.R~~]]	+	== References ==
-	[[Category: Zheng, W]]	+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Homo sapiens]]
		+	[[Category: Large Structures]]
		+	[[Category: Fu TM]]
		+	[[Category: Holt JR]]
		+	[[Category: Zheng W]]

OCA at 17:13, 19 October 2022

OCA — Wed, 19 Oct 2022 17:13:57 GMT

←Older revision		Revision as of 17:13, 19 October 2022
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 8ehw is ON HOLD	+	The entry 8ehw is ON HOLD until Paper Publication

	Authors: Zheng, W., Fu, T.M., Holt, J.R.		Authors: Zheng, W., Fu, T.M., Holt, J.R.

OCA: Protected "8ehw" [edit=sysop:move=sysop]

OCA — Wed, 21 Sep 2022 06:38:31 GMT

Protected "8ehw" [edit=sysop:move=sysop]

←Older revision

Revision as of 06:38, 21 September 2022

OCA: New page: '''Unreleased structure''' The entry 8ehw is ON HOLD Authors: Zheng, W., Fu, T.M., Holt, J.R. Description: cryo-EM structure of TMEM63A in nanodisc Category: Unreleased Structures ...

OCA — Wed, 21 Sep 2022 06:38:30 GMT

New page: '''Unreleased structure''' The entry 8ehw is ON HOLD Authors: Zheng, W., Fu, T.M., Holt, J.R. Description: cryo-EM structure of TMEM63A in nanodisc Category: Unreleased Structures ...

New page

'''Unreleased structure'''

The entry 8ehw is ON HOLD

Authors: Zheng, W., Fu, T.M., Holt, J.R.

Description: cryo-EM structure of TMEM63A in nanodisc
[[Category: Unreleased Structures]]
[[Category: Fu, T.M]]
[[Category: Holt, J.R]]
[[Category: Zheng, W]]

8ehw - Revision history

OCA at 05:53, 23 August 2023

OCA at 17:13, 19 October 2022

OCA: Protected "8ehw" [edit=sysop:move=sysop]

OCA: New page: '''Unreleased structure''' The entry 8ehw is ON HOLD Authors: Zheng, W., Fu, T.M., Holt, J.R. Description: cryo-EM structure of TMEM63A in nanodisc Category: Unreleased Structures ...