8ejm - Revision history

OCA at 10:21, 25 October 2023

OCA — Wed, 25 Oct 2023 10:21:48 GMT

←Older revision		Revision as of 10:21, 25 October 2023
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	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[8ejm]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8EJM OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8EJM FirstGlance]. <br>		<table><tr><td colspan='2'>[[8ejm]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8EJM OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8EJM FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.8Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8ejm FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8ejm OCA], [https://pdbe.org/8ejm PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8ejm RCSB], [https://www.ebi.ac.uk/pdbsum/8ejm PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8ejm ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8ejm FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8ejm OCA], [https://pdbe.org/8ejm PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8ejm RCSB], [https://www.ebi.ac.uk/pdbsum/8ejm PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8ejm ProSAT]</span></td></tr>
	</table>		</table>
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	</div>		</div>
	<div class="pdbe-citations 8ejm" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 8ejm" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Helicase 3D structures\|Helicase 3D structures]]
	== References ==		== References ==
	<references/>		<references/>

OCA at 10:11, 14 December 2022

OCA — Wed, 14 Dec 2022 10:11:41 GMT

←Older revision		Revision as of 10:11, 14 December 2022
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-	'''Unreleased structure'''

-	~~The entry~~ 8ejm is ~~ON HOLD~~	+	==Crystal structure of human DEAH-box helicase DHX15 in complex with SUGP1 G-patch==
		+	<StructureSection load='8ejm' size='340' side='right'caption='[[8ejm]], [[Resolution\|resolution]] 1.80Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[8ejm]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8EJM OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8EJM FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8ejm FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8ejm OCA], [https://pdbe.org/8ejm PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8ejm RCSB], [https://www.ebi.ac.uk/pdbsum/8ejm PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8ejm ProSAT]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/DHX15_HUMAN DHX15_HUMAN] Pre-mRNA processing factor involved in disassembly of spliceosomes after the release of mature mRNA. In cooperation with TFIP11 seem to be involved in the transition of the U2, U5 and U6 snRNP-containing IL complex to the snRNP-free IS complex leading to efficient debranching and turnover of excised introns.<ref>PMID:19103666</ref>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	SF3B1 is the most frequently mutated spliceosomal gene in cancer. Several hotspot mutations are known to disrupt the interaction of SF3B1 with another splicing factor, SUGP1, resulting in the RNA missplicing that characterizes mutant SF3B1 cancers. Properties of SUGP1, especially the presence of a G-patch motif, a structure known to function by activating DEAH-box RNA helicases, suggest the requirement of such an enzyme in SUGP1 function in splicing. However, the identity of this putative helicase has remained an important unanswered question. Here, using a variety of protein-protein interaction assays, we identify DHX15 as the critical helicase. We further show that depletion of DHX15 or expression of any of several DHX15 mutants, including one implicated in acute myeloid leukemia, partially recapitulates the splicing defects of mutant SF3B1. Moreover, a DHX15-SUGP1 G-patch fusion protein is able to incorporate into the spliceosome to rescue the splicing defects of mutant SF3B1. We also present the crystal structure of the human DHX15-SUGP1 G-patch complex, which reveals the molecular basis of their direct interaction. Our data thus demonstrate that DHX15 is the RNA helicase that functions with SUGP1 and additionally provide important insight into how mutant SF3B1 disrupts splicing in cancer.

-	~~Authors~~:	+	DHX15 is involved in SUGP1-mediated RNA missplicing by mutant SF3B1 in cancer.,Zhang J, Huang J, Xu K, Xing P, Huang Y, Liu Z, Tong L, Manley JL Proc Natl Acad Sci U S A. 2022 Dec 6;119(49):e2216712119. doi: , 10.1073/pnas.2216712119. Epub 2022 Dec 2. PMID:36459648<ref>PMID:36459648</ref>

-	~~Description~~:	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased~~ Structures]]	+	</div>
		+	<div class="pdbe-citations 8ejm" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Homo sapiens]]
		+	[[Category: Large Structures]]
		+	[[Category: Huang J]]
		+	[[Category: Tong L]]

OCA: Protected "8ejm" [edit=sysop:move=sysop]

OCA — Wed, 28 Sep 2022 04:45:34 GMT

Protected "8ejm" [edit=sysop:move=sysop]

←Older revision

Revision as of 04:45, 28 September 2022

OCA: New page: '''Unreleased structure''' The entry 8ejm is ON HOLD Authors: Description: Category: Unreleased Structures

OCA — Wed, 28 Sep 2022 04:45:33 GMT

New page: '''Unreleased structure''' The entry 8ejm is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 8ejm is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]