OCA: Protected "8ema" [edit=sysop:move=sysop]

OCA — Wed, 16 Nov 2022 20:17:55 GMT

Protected "8ema" [edit=sysop:move=sysop]

←Older revision

Revision as of 20:17, 16 November 2022

OCA: New page: ==mouse full length B cell receptor== == Structural highlights == ...

OCA — Wed, 16 Nov 2022 20:17:53 GMT

New page: ==mouse full length B cell receptor== <StructureSection load='8ema' size='340' side='right'caption='8ema, resolution 8.20Å' scene=''> == Structural highlights == ...

New page

==mouse full length B cell receptor==
<StructureSection load='8ema' size='340' side='right'caption='[[8ema]], [[Resolution|resolution]] 8.20Å' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[8ema]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Aliivibrio_fischeri Aliivibrio fischeri] and [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8EMA OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8EMA FirstGlance]. <br>
</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8ema FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8ema OCA], [https://pdbe.org/8ema PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8ema RCSB], [https://www.ebi.ac.uk/pdbsum/8ema PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8ema ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/HVM49_MOUSE HVM49_MOUSE] [https://www.uniprot.org/uniprot/IGHM_MOUSE IGHM_MOUSE]
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
The B-cell antigen receptor (BCR) is composed of a membrane-bound immunoglobulin (mIg) of class M, D, G, E or A for antigen recognition(1-3) and a disulfide-linked Igalpha and Igbeta heterodimer (Igalpha/beta) that functions as the signalling entity through their intracellular immunoreceptor tyrosine-based activation motifs (ITAMs)(4,5). The organizing principle of the BCR remains elusive. Here we report cryogenic electron microscopy structures of mouse full-length IgM BCR at 8.2 A resolution and its Fab-deleted form at 3.3 A resolution. At the ectodomain (ECD), the Igalpha/beta heterodimer mainly uses Igalpha to associate with Cmicro3-Cmicro4 domains of one heavy chain (microHC) while leaving the other heavy chain (microHC') empty. The transmembrane domain (TMD) helices of the two microHCs interact with those of the Igalpha/beta heterodimer to form a tight 4-helix bundle. The asymmetry at the TMD prevents the recruitment of two Igalpha/beta heterodimers. Surprisingly, the connecting peptide between the ECD and TMD of microHC intervenes in between those of Igalpha and Igbeta to guide the TMD assembly through striking charge complementarity. Weaker but distinct density for the Igbeta ITAMs nestles next to the TMD, suggesting potential autoinhibition of ITAM phosphorylation. Interfacial analyses suggest that all BCR classes utilize a general organizational architecture. Our studies provide a structural platform for understanding B-cell signalling and designing rational therapies against BCR-mediated diseases.

Structural principles of B-cell antigen receptor assembly.,Dong Y, Pi X, Bartels-Burgahn F, Saltukoglu D, Liang Z, Yang J, Alt FW, Reth M, Wu H Nature. 2022 Oct 13. pii: 10.1038/s41586-022-05412-7. doi:, 10.1038/s41586-022-05412-7. PMID:36228656<ref>PMID:36228656</ref>

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 8ema" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Aliivibrio fischeri]]
[[Category: Large Structures]]
[[Category: Mus musculus]]
[[Category: Michael R]]
[[Category: Xiong P]]
[[Category: Ying D]]

8ema - Revision history

OCA: Protected "8ema" [edit=sysop:move=sysop]

OCA: New page: ==mouse full length B cell receptor== == Structural highlights == ...