OCA at 12:37, 26 July 2023

OCA — Wed, 26 Jul 2023 12:37:45 GMT

←Older revision		Revision as of 12:37, 26 July 2023
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 8eon is ~~ON HOLD~~	+	==Pseudomonas phage E217 baseplate complex==
		+	<StructureSection load='8eon' size='340' side='right'caption='[[8eon]], [[Resolution\|resolution]] 3.60Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[8eon]] is a 48 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_phage_vB_PaeM_E217 Pseudomonas phage vB_PaeM_E217]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8EON OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8EON FirstGlance]. <br>
		+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution\|Resolution]] 3.6Å</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8eon FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8eon OCA], [https://pdbe.org/8eon PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8eon RCSB], [https://www.ebi.ac.uk/pdbsum/8eon PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8eon ProSAT]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/A0A6G9LFR0_9CAUD A0A6G9LFR0_9CAUD]
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	E217 is a Pseudomonas phage used in an experimental cocktail to eradicate cystic fibrosis-associated Pseudomonas aeruginosa. Here, we describe the structure of the whole E217 virion before and after DNA ejection at 3.1 A and 4.5 A resolution, respectively, determined using cryogenic electron microscopy (cryo-EM). We identify and build de novo structures for 19 unique E217 gene products, resolve the tail genome-ejection machine in both extended and contracted states, and decipher the complete architecture of the baseplate formed by 66 polypeptide chains. We also determine that E217 recognizes the host O-antigen as a receptor, and we resolve the N-terminal portion of the O-antigen-binding tail fiber. We propose that E217 design principles presented in this paper are conserved across PB1-like Myoviridae phages of the Pbunavirus genus that encode a ~1.4 MDa baseplate, dramatically smaller than the coliphage T4.

-	~~Authors:~~ Li, F., ~~Cingolani~~, G.~~, Hou, C~~.	+	High-resolution cryo-EM structure of the Pseudomonas bacteriophage E217.,Li F, Hou CD, Lokareddy RK, Yang R, Forti F, Briani F, Cingolani G Nat Commun. 2023 Jul 8;14(1):4052. doi: 10.1038/s41467-023-39756-z. PMID:37422479<ref>PMID:37422479</ref>

-	~~Description~~: ~~Pseudomonas phage E217 baseplate complex~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased~~ Structures]]	+	</div>
-	[[Category: ~~Cingolani, G~~]]	+	<div class="pdbe-citations 8eon" style="background-color:#fffaf0;"></div>
-	[[Category: ~~Li, F~~]]	+	== References ==
-	[[Category: Hou, C]]	+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Pseudomonas phage vB_PaeM_E217]]
		+	[[Category: Cingolani G]]
		+	[[Category: Hou C]]
		+	[[Category: Li F]]

OCA at 09:21, 1 February 2023

OCA — Wed, 01 Feb 2023 09:21:04 GMT

←Older revision		Revision as of 09:21, 1 February 2023
Line 5:		Line 5:
	Authors: Li, F., Cingolani, G., Hou, C.		Authors: Li, F., Cingolani, G., Hou, C.

-	Description: ~~In situ cryo-EM structure of~~ Pseudomonas phage E217 ~~tail~~ baseplate ~~in C3 map~~	+	Description: Pseudomonas phage E217 baseplate complex
	[[Category: Unreleased Structures]]		[[Category: Unreleased Structures]]
	[[Category: Cingolani, G]]		[[Category: Cingolani, G]]
	[[Category: Li, F]]		[[Category: Li, F]]
	[[Category: Hou, C]]		[[Category: Hou, C]]

OCA: Protected "8eon" [edit=sysop:move=sysop]

OCA — Wed, 19 Oct 2022 17:19:56 GMT

Protected "8eon" [edit=sysop:move=sysop]

←Older revision

Revision as of 17:19, 19 October 2022

OCA: New page: '''Unreleased structure''' The entry 8eon is ON HOLD Authors: Li, F., Cingolani, G., Hou, C. Description: In situ cryo-EM structure of Pseudomonas phage E217 tail baseplate in C3 map [...

OCA — Wed, 19 Oct 2022 17:19:55 GMT

New page: '''Unreleased structure''' The entry 8eon is ON HOLD Authors: Li, F., Cingolani, G., Hou, C. Description: In situ cryo-EM structure of Pseudomonas phage E217 tail baseplate in C3 map [...

New page

'''Unreleased structure'''

The entry 8eon is ON HOLD

Authors: Li, F., Cingolani, G., Hou, C.

Description: In situ cryo-EM structure of Pseudomonas phage E217 tail baseplate in C3 map
[[Category: Unreleased Structures]]
[[Category: Cingolani, G]]
[[Category: Li, F]]
[[Category: Hou, C]]

8eon - Revision history

OCA at 12:37, 26 July 2023

OCA at 09:21, 1 February 2023

OCA: Protected "8eon" [edit=sysop:move=sysop]

OCA: New page: '''Unreleased structure''' The entry 8eon is ON HOLD Authors: Li, F., Cingolani, G., Hou, C. Description: In situ cryo-EM structure of Pseudomonas phage E217 tail baseplate in C3 map [...