8et9 - Revision history

OCA at 07:35, 12 July 2023

OCA — Wed, 12 Jul 2023 07:35:46 GMT

←Older revision		Revision as of 07:35, 12 July 2023
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	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[8et9]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8ET9 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8ET9 FirstGlance]. <br>		<table><tr><td colspan='2'>[[8et9]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8ET9 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8ET9 FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=WRF:1-methyl-4-phenylpyridin-1-ium'>WRF</scene></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution\|Resolution]] 3.61Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=WRF:1-methyl-4-phenylpyridin-1-ium'>WRF</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8et9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8et9 OCA], [https://pdbe.org/8et9 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8et9 RCSB], [https://www.ebi.ac.uk/pdbsum/8et9 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8et9 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8et9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8et9 OCA], [https://pdbe.org/8et9 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8et9 RCSB], [https://www.ebi.ac.uk/pdbsum/8et9 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8et9 ProSAT]</span></td></tr>
	</table>		</table>

OCA at 05:42, 31 May 2023

OCA — Wed, 31 May 2023 05:42:51 GMT

←Older revision		Revision as of 05:42, 31 May 2023
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-	'''Unreleased structure'''

-	~~The entry~~ 8et9 is ~~ON HOLD~~	+	==Cryo-EM structure of the organic cation transporter 2 in complex with 1-methyl-4-phenylpyridinium==
		+	<StructureSection load='8et9' size='340' side='right'caption='[[8et9]], [[Resolution\|resolution]] 3.61Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[8et9]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8ET9 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8ET9 FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=WRF:1-methyl-4-phenylpyridin-1-ium'>WRF</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8et9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8et9 OCA], [https://pdbe.org/8et9 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8et9 RCSB], [https://www.ebi.ac.uk/pdbsum/8et9 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8et9 ProSAT]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	A wide range of endogenous and xenobiotic organic ions require facilitated transport systems to cross the plasma membrane for their disposition (1, 2) . In mammals, organic cation transporter subtypes 1 and 2 (OCT1 and OCT2, also known as SLC22A1 and SLC22A2, respectively) are polyspecific transporters responsible for the uptake and clearance of structurally diverse cationic compounds in the liver and kidneys, respectively (3, 4) . Notably, it is well established that human OCT1 and OCT2 play central roles in the pharmacokinetics, pharmacodynamics, and drug-drug interactions (DDI) of many prescription medications, including metformin (5, 6) . Despite their importance, the basis of polyspecific cationic drug recognition and the alternating access mechanism for OCTs have remained a mystery. Here, we present four cryo-EM structures of apo, substrate-bound, and drug-bound OCT1 and OCT2 in outward-facing and outward-occluded states. Together with functional experiments, in silico docking, and molecular dynamics simulations, these structures uncover general principles of organic cation recognition by OCTs and illuminate unexpected features of the OCT alternating access mechanism. Our findings set the stage for a comprehensive structure-based understanding of OCT-mediated DDI, which will prove critical in the preclinical evaluation of emerging therapeutics.

-	~~Authors~~:	+	Molecular basis of polyspecific drug binding and transport by OCT1 and OCT2.,Suo Y, Wright NJ, Guterres H, Fedor JG, Butay KJ, Borgnia MJ, Im W, Lee SY bioRxiv. 2023 Mar 16:2023.03.15.532610. doi: 10.1101/2023.03.15.532610. Preprint. PMID:36993738<ref>PMID:36993738</ref>

-	~~Description~~:	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased~~ Structures]]	+	</div>
		+	<div class="pdbe-citations 8et9" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Homo sapiens]]
		+	[[Category: Large Structures]]
		+	[[Category: Lee S-Y]]
		+	[[Category: Suo Y]]
		+	[[Category: Wright NJ]]

OCA at 05:21, 3 November 2022

OCA — Thu, 03 Nov 2022 05:21:51 GMT

←Older revision		Revision as of 05:21, 3 November 2022
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	The entry 8et9 is ON HOLD		The entry 8et9 is ON HOLD

-	Authors: ~~Suo, Y., Wright, N.J., Lee, S.-Y.~~	+	Authors:

-	Description: ~~Cryo-EM structure of the organic cation transporter 2 in complex with 1-methyl-4-phenylpyridinium~~	+	Description:
	[[Category: Unreleased Structures]]		[[Category: Unreleased Structures]]
-	[[Category: Suo, Y]]
-	[[Category: Lee, S.-Y]]
-	[[Category: Wright, N.J]]

OCA: Protected "8et9" [edit=sysop:move=sysop]

OCA — Wed, 26 Oct 2022 04:03:06 GMT

Protected "8et9" [edit=sysop:move=sysop]

←Older revision

Revision as of 04:03, 26 October 2022

OCA: New page: '''Unreleased structure''' The entry 8et9 is ON HOLD Authors: Suo, Y., Wright, N.J., Lee, S.-Y. Description: Cryo-EM structure of the organic cation transporter 2 in complex with 1-met...

OCA — Wed, 26 Oct 2022 04:03:05 GMT

New page: '''Unreleased structure''' The entry 8et9 is ON HOLD Authors: Suo, Y., Wright, N.J., Lee, S.-Y. Description: Cryo-EM structure of the organic cation transporter 2 in complex with 1-met...

New page

'''Unreleased structure'''

The entry 8et9 is ON HOLD

Authors: Suo, Y., Wright, N.J., Lee, S.-Y.

Description: Cryo-EM structure of the organic cation transporter 2 in complex with 1-methyl-4-phenylpyridinium
[[Category: Unreleased Structures]]
[[Category: Suo, Y]]
[[Category: Lee, S.-Y]]
[[Category: Wright, N.J]]