8euh - Revision history

OCA at 10:23, 25 October 2023

OCA — Wed, 25 Oct 2023 10:23:56 GMT

←Older revision		Revision as of 10:23, 25 October 2023
Line 4:		Line 4:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[8euh]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_sp. Pseudomonas sp.]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8EUH OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8EUH FirstGlance]. <br>		<table><tr><td colspan='2'>[[8euh]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_sp. Pseudomonas sp.]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8EUH OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8EUH FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene>, <scene name='pdbligand=XGE:alpha-TERPINEOL'>XGE</scene></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene>, <scene name='pdbligand=XGE:alpha-TERPINEOL'>XGE</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8euh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8euh OCA], [https://pdbe.org/8euh PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8euh RCSB], [https://www.ebi.ac.uk/pdbsum/8euh PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8euh ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8euh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8euh OCA], [https://pdbe.org/8euh PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8euh RCSB], [https://www.ebi.ac.uk/pdbsum/8euh PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8euh ProSAT]</span></td></tr>
	</table>		</table>

OCA at 12:44, 22 February 2023

OCA — Wed, 22 Feb 2023 12:44:29 GMT

←Older revision		Revision as of 12:44, 22 February 2023
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 8euh is ~~ON HOLD until Paper~~ Publication	+	==cytochrome P450terp (cyp108A1) bound to alpha-terpineol==
		+	<StructureSection load='8euh' size='340' side='right'caption='[[8euh]], [[Resolution\|resolution]] 2.00Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[8euh]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_sp. Pseudomonas sp.]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8EUH OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8EUH FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene>, <scene name='pdbligand=XGE:alpha-TERPINEOL'>XGE</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8euh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8euh OCA], [https://pdbe.org/8euh PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8euh RCSB], [https://www.ebi.ac.uk/pdbsum/8euh PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8euh ProSAT]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/CPXL_PSESP CPXL_PSESP] Catalyzes the hydroxylation of alpha-terpineol.
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Despite being one of the most well-studied aspects of cytochrome P450 chemistry, important questions remain regarding the nature and ubiquity of allosteric regulation of catalysis. The crystal structure of a bacterial P450, P450terp, in the presence of substrate reveals two binding sites, one above the heme in position for regioselective hydroxylation and another in the substrate access channel. Unlike many bacterial P450s, P450terp does not exhibit an open to closed conformational change when substrate binds; instead, P450terp uses the second substrate molecule to hold the first substrate molecule in position for catalysis. Spectral titrations clearly show that substrate binding to P450terp is cooperative with a Hill coefficient of 1.4 and is supported by isothermal titration calorimetry. The importance of the allosteric site was explored by a series of mutations that weaken the second site and that help hold the first substrate in position for proper catalysis. We further measured the coupling efficiency of both the wild-type (WT) enzyme and the mutant enzymes. While the WT enzyme exhibits 97% efficiency, each of the variants showed lower catalytic efficiency. Additionally, the variants show decreased spin shifts upon binding of substrate. These results are the first clear example of positive homotropic allostery in a class 1 bacterial P450 with its natural substrate. Combined with our recent results from P450cam showing complex substrate allostery and conformational dynamics, our present study with P450terp indicates that bacterial P450s may not be as simple as once thought and share complex substrate binding properties usually associated with only mammalian P450s.

-	~~Authors~~:	+	Cooperative Substrate Binding Controls Catalysis in Bacterial Cytochrome P450terp (CYP108A1).,Gable JA, Poulos TL, Follmer AH J Am Chem Soc. 2023 Feb 13. doi: 10.1021/jacs.2c12388. PMID:36779970<ref>PMID:36779970</ref>

-	~~Description~~:	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased~~ Structures]]	+	</div>
		+	<div class="pdbe-citations 8euh" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Pseudomonas sp]]
		+	[[Category: Follmer AH]]
		+	[[Category: Gable JA]]
		+	[[Category: Poulos TL]]

OCA at 08:32, 30 November 2022

OCA — Wed, 30 Nov 2022 08:32:31 GMT

←Older revision		Revision as of 08:32, 30 November 2022
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 8euh is ON HOLD	+	The entry 8euh is ON HOLD until Paper Publication

	Authors:		Authors:

OCA: Protected "8euh" [edit=sysop:move=sysop]

OCA — Wed, 26 Oct 2022 04:05:37 GMT

Protected "8euh" [edit=sysop:move=sysop]

←Older revision

Revision as of 04:05, 26 October 2022

OCA: New page: '''Unreleased structure''' The entry 8euh is ON HOLD Authors: Description: Category: Unreleased Structures

OCA — Wed, 26 Oct 2022 04:05:36 GMT

New page: '''Unreleased structure''' The entry 8euh is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 8euh is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]