8ev9 - Revision history

OCA at 05:26, 9 August 2023

OCA — Wed, 09 Aug 2023 05:26:57 GMT

←Older revision		Revision as of 05:26, 9 August 2023
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-	'''Unreleased structure'''

-	~~The entry~~ 8ev9 is ~~ON HOLD~~ ~~until Paper~~ Publication	+	==Cryo-EM structure of cGMP bound truncated human CNGA3/CNGB3 channel in lipid nanodisc, transition state 1==
		+	<StructureSection load='8ev9' size='340' side='right'caption='[[8ev9]], [[Resolution\|resolution]] 3.33Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[8ev9]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8EV9 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8EV9 FirstGlance]. <br>
		+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution\|Resolution]] 3.33Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=PCG:CYCLIC+GUANOSINE+MONOPHOSPHATE'>PCG</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8ev9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8ev9 OCA], [https://pdbe.org/8ev9 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8ev9 RCSB], [https://www.ebi.ac.uk/pdbsum/8ev9 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8ev9 ProSAT]</span></td></tr>
		+	</table>
		+	== Disease ==
		+	[https://www.uniprot.org/uniprot/CNGB3_HUMAN CNGB3_HUMAN] Stargardt disease;Progressive cone dystrophy;Achromatopsia. The disease is caused by variants affecting the gene represented in this entry. The disease is caused by variants affecting the gene represented in this entry.
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/CNGB3_HUMAN CNGB3_HUMAN] Visual signal transduction is mediated by a G-protein coupled cascade using cGMP as second messenger. This protein can be activated by cGMP which leads to an opening of the cation channel and thereby causing a depolarization of rod photoreceptors. Induced a flickering channel gating, weakened the outward rectification in the presence of extracellular calcium, increased sensitivity for L-cis diltiazem and enhanced the cAMP efficiency of the channel when coexpressed with CNGA3 (By similarity). Essential for the generation of light-evoked electrical responses in the red-, green- and blue sensitive cones.<ref>PMID:10888875</ref>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Cyclic nucleotide-gated (CNG) channels transduce chemical signals into electrical signals in sensory receptors and neurons. They are activated by cGMP or cAMP, which bind to the cyclic nucleotide-binding domain (CNBD) to open a gate located 50-60 A away in the central cavity. Structures of closed and open vertebrate CNG channels have been solved, but the conformational landscape of this allosteric gating remains to be elucidated and enriched. Here, we report structures of the cGMP-activated human cone photoreceptor CNGA3/CNGB3 channel in closed, intermediate, pre-open and open states in detergent or lipid nanodisc, all with fully bound cGMP. The pre-open and open states are obtained only in the lipid nanodisc, suggesting a critical role of lipids in tuning the energetic landscape of CNGA3/CNGB3 activation. The different states exhibit subunit-unique, incremental and distinct conformational rearrangements that originate in the CNBD, propagate through the gating ring to the transmembrane domain, and gradually open the S6 cavity gate. Our work illustrates a spatial conformational-change wave of allosteric gating of a vertebrate CNG channel by its natural ligand and provides an expanded framework for studying CNG properties and channelopathy.

-	~~Authors: Hu~~, Z., ~~Yang~~, J.	+	Conformational trajectory of allosteric gating of the human cone photoreceptor cyclic nucleotide-gated channel.,Hu Z, Zheng X, Yang J Nat Commun. 2023 Jul 18;14(1):4284. doi: 10.1038/s41467-023-39971-8. PMID:37463923<ref>PMID:37463923</ref>

-	~~Description: Cryo-EM structure of cGMP bound truncated human CNGA3~~/~~CNGB3 channel in lipid nanodisc~~, ~~transition state 1~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased Structures~~]]	+	</div>
-	[[Category: ~~Yang, J~~]]	+	<div class="pdbe-citations 8ev9" style="background-color:#fffaf0;"></div>
-	[[Category: Hu, Z]]	+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Homo sapiens]]
		+	[[Category: Large Structures]]
		+	[[Category: Hu Z]]
		+	[[Category: Yang J]]

OCA at 07:46, 19 April 2023

OCA — Wed, 19 Apr 2023 07:46:59 GMT

←Older revision		Revision as of 07:46, 19 April 2023
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 8ev9 is ON HOLD until ~~2024-04-20~~	+	The entry 8ev9 is ON HOLD until Paper Publication

	Authors: Hu, Z., Yang, J.		Authors: Hu, Z., Yang, J.
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	Description: Cryo-EM structure of cGMP bound truncated human CNGA3/CNGB3 channel in lipid nanodisc, transition state 1		Description: Cryo-EM structure of cGMP bound truncated human CNGA3/CNGB3 channel in lipid nanodisc, transition state 1
	[[Category: Unreleased Structures]]		[[Category: Unreleased Structures]]
-	[[Category: Hu, Z]]
	[[Category: Yang, J]]		[[Category: Yang, J]]
		+	[[Category: Hu, Z]]

OCA at 04:55, 25 January 2023

OCA — Wed, 25 Jan 2023 04:55:45 GMT

←Older revision		Revision as of 04:55, 25 January 2023
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 8ev9 is ON HOLD	+	The entry 8ev9 is ON HOLD until 2024-04-20

	Authors: Hu, Z., Yang, J.		Authors: Hu, Z., Yang, J.

OCA at 07:33, 14 December 2022

OCA — Wed, 14 Dec 2022 07:33:39 GMT

←Older revision		Revision as of 07:33, 14 December 2022
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 8ev9 is ON HOLD ~~until 2024-04-20~~	+	The entry 8ev9 is ON HOLD

	Authors: Hu, Z., Yang, J.		Authors: Hu, Z., Yang, J.
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	Description: Cryo-EM structure of cGMP bound truncated human CNGA3/CNGB3 channel in lipid nanodisc, transition state 1		Description: Cryo-EM structure of cGMP bound truncated human CNGA3/CNGB3 channel in lipid nanodisc, transition state 1
	[[Category: Unreleased Structures]]		[[Category: Unreleased Structures]]
-	[[Category: Yang, J]]
	[[Category: Hu, Z]]		[[Category: Hu, Z]]
		+	[[Category: Yang, J]]

OCA at 08:08, 24 November 2022

OCA — Thu, 24 Nov 2022 08:08:30 GMT

←Older revision		Revision as of 08:08, 24 November 2022
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 8ev9 is ON HOLD	+	The entry 8ev9 is ON HOLD until 2024-04-20

	Authors: Hu, Z., Yang, J.		Authors: Hu, Z., Yang, J.

OCA: Protected "8ev9" [edit=sysop:move=sysop]

OCA — Wed, 26 Oct 2022 04:07:06 GMT

Protected "8ev9" [edit=sysop:move=sysop]

←Older revision

Revision as of 04:07, 26 October 2022

OCA: New page: '''Unreleased structure''' The entry 8ev9 is ON HOLD Authors: Hu, Z., Yang, J. Description: Cryo-EM structure of cGMP bound truncated human CNGA3/CNGB3 channel in lipid nanodisc, trans...

OCA — Wed, 26 Oct 2022 04:07:05 GMT

New page: '''Unreleased structure''' The entry 8ev9 is ON HOLD Authors: Hu, Z., Yang, J. Description: Cryo-EM structure of cGMP bound truncated human CNGA3/CNGB3 channel in lipid nanodisc, trans...

New page

'''Unreleased structure'''

The entry 8ev9 is ON HOLD

Authors: Hu, Z., Yang, J.

Description: Cryo-EM structure of cGMP bound truncated human CNGA3/CNGB3 channel in lipid nanodisc, transition state 1
[[Category: Unreleased Structures]]
[[Category: Yang, J]]
[[Category: Hu, Z]]