OCA at 09:53, 15 March 2023

OCA — Wed, 15 Mar 2023 09:53:04 GMT

←Older revision		Revision as of 09:53, 15 March 2023
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-	'''Unreleased structure'''

-	~~The entry~~ 8f01 is ~~ON HOLD until Paper~~ Publication	+	==Thaumatin Anomalous Dataset at 293 K and 7.1 keV==
		+	<StructureSection load='8f01' size='340' side='right'caption='[[8f01]], [[Resolution\|resolution]] 1.80Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[8f01]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Thaumatococcus_daniellii Thaumatococcus daniellii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8F01 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8F01 FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=K:POTASSIUM+ION'>K</scene>, <scene name='pdbligand=TLA:L(+)-TARTARIC+ACID'>TLA</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8f01 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8f01 OCA], [https://pdbe.org/8f01 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8f01 RCSB], [https://www.ebi.ac.uk/pdbsum/8f01 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8f01 ProSAT]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/THM1_THADA THM1_THADA] Taste-modifying protein; intensely sweet-tasting. It is 100000 times sweeter than sucrose on a molar basis.
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	X-ray crystallography has been invaluable in delivering structural information about proteins. Previously, an approach has been developed that allows high-quality X-ray diffraction data to be obtained from protein crystals at and above room temperature. Here, this previous work is built on and extended by showing that high-quality anomalous signal can be obtained from single protein crystals using diffraction data collected at 220 K up to physiological temperatures. The anomalous signal can be used to directly determine the structure of a protein, i.e. to phase the data, as is routinely performed under cryoconditions. This ability is demonstrated by obtaining diffraction data from model lysozyme, thaumatin and proteinase K crystals, the anomalous signal from which allowed their structures to be solved experimentally at 7.1 keV X-ray energy and at room temperature with relatively low data redundancy. It is also demonstrated that the anomalous signal from diffraction data obtained at 310 K (37 degrees C) can be used to solve the structure of proteinase K and to identify ordered ions. The method provides useful anomalous signal at temperatures down to 220 K, resulting in an extended crystal lifetime and increased data redundancy. Finally, we show that useful anomalous signal can be obtained at room temperature using X-rays of 12 keV energy as typically used for routine data collection, allowing this type of experiment to be carried out at widely accessible synchrotron beamline energies and enabling the simultaneous extraction of high-resolution data and anomalous signal. With the recent emphasis on obtaining conformational ensemble information for proteins, the high resolution of the data allows such ensembles to be built, while the anomalous signal allows the structure to be experimentally solved, ions to be identified, and water molecules and ions to be differentiated. Because bound metal-, phosphorus- and sulfur-containing ions all have anomalous signal, obtaining anomalous signal across temperatures and up to physiological temperatures will provide a more complete description of protein conformational ensembles, function and energetics.

-	~~Authors: Doukov~~, T., ~~Yabukarski~~, F., ~~Herschlag, D~~.	+	Obtaining anomalous and ensemble information from protein crystals from 220 K up to physiological temperatures.,Doukov T, Herschlag D, Yabukarski F Acta Crystallogr D Struct Biol. 2023 Mar 1;79(Pt 3):212-223. doi: , 10.1107/S205979832300089X. Epub 2023 Feb 27. PMID:36876431<ref>PMID:36876431</ref>

-	~~Description: Thaumatin Anomalous Dataset at 293 K and 7~~.~~1 keV~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased~~ Structures]]	+	</div>
-	[[Category: ~~Doukov, T~~]]	+	<div class="pdbe-citations 8f01" style="background-color:#fffaf0;"></div>
-	[[Category: ~~Yabukarski, F~~]]	+	== References ==
-	[[Category: Herschlag, D]]	+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Thaumatococcus daniellii]]
		+	[[Category: Doukov T]]
		+	[[Category: Herschlag D]]
		+	[[Category: Yabukarski F]]

OCA at 07:35, 14 December 2022

OCA — Wed, 14 Dec 2022 07:35:08 GMT

←Older revision		Revision as of 07:35, 14 December 2022
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 8f01 is ON HOLD	+	The entry 8f01 is ON HOLD until Paper Publication

	Authors: Doukov, T., Yabukarski, F., Herschlag, D.		Authors: Doukov, T., Yabukarski, F., Herschlag, D.
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	[[Category: Unreleased Structures]]		[[Category: Unreleased Structures]]
	[[Category: Doukov, T]]		[[Category: Doukov, T]]
-	[[Category: Herschlag, D]]
	[[Category: Yabukarski, F]]		[[Category: Yabukarski, F]]
		+	[[Category: Herschlag, D]]

OCA: Protected "8f01" [edit=sysop:move=sysop]

OCA — Wed, 09 Nov 2022 05:08:33 GMT

Protected "8f01" [edit=sysop:move=sysop]

←Older revision

Revision as of 05:08, 9 November 2022

OCA: New page: '''Unreleased structure''' The entry 8f01 is ON HOLD Authors: Doukov, T., Yabukarski, F., Herschlag, D. Description: Thaumatin Anomalous Dataset at 293 K and 7.1 keV [[Category: Unrele...

OCA — Wed, 09 Nov 2022 05:08:32 GMT

New page: '''Unreleased structure''' The entry 8f01 is ON HOLD Authors: Doukov, T., Yabukarski, F., Herschlag, D. Description: Thaumatin Anomalous Dataset at 293 K and 7.1 keV [[Category: Unrele...

New page

'''Unreleased structure'''

The entry 8f01 is ON HOLD

Authors: Doukov, T., Yabukarski, F., Herschlag, D.

Description: Thaumatin Anomalous Dataset at 293 K and 7.1 keV
[[Category: Unreleased Structures]]
[[Category: Doukov, T]]
[[Category: Herschlag, D]]
[[Category: Yabukarski, F]]

8f01 - Revision history

OCA at 09:53, 15 March 2023

OCA at 07:35, 14 December 2022

OCA: Protected "8f01" [edit=sysop:move=sysop]

OCA: New page: '''Unreleased structure''' The entry 8f01 is ON HOLD Authors: Doukov, T., Yabukarski, F., Herschlag, D. Description: Thaumatin Anomalous Dataset at 293 K and 7.1 keV [[Category: Unrele...