8f5v - Revision history

OCA at 08:28, 11 October 2023

OCA — Wed, 11 Oct 2023 08:28:18 GMT

←Older revision		Revision as of 08:28, 11 October 2023
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	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[8f5v]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Mycobacterium_tuberculosis Mycobacterium tuberculosis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8F5V OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8F5V FirstGlance]. <br>		<table><tr><td colspan='2'>[[8f5v]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Mycobacterium_tuberculosis Mycobacterium tuberculosis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8F5V OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8F5V FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=XGZ:Mycothiol'>XGZ</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.45Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=XGZ:Mycothiol'>XGZ</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8f5v FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8f5v OCA], [https://pdbe.org/8f5v PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8f5v RCSB], [https://www.ebi.ac.uk/pdbsum/8f5v PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8f5v ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8f5v FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8f5v OCA], [https://pdbe.org/8f5v PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8f5v RCSB], [https://www.ebi.ac.uk/pdbsum/8f5v PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8f5v ProSAT]</span></td></tr>
	</table>		</table>

OCA at 06:30, 7 April 2023

OCA — Fri, 07 Apr 2023 06:30:50 GMT

←Older revision		Revision as of 06:30, 7 April 2023
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-	'''Unreleased structure'''

-	~~The entry~~ 8f5v is ~~ON HOLD until Paper~~ Publication	+	==Crystal structure of Mycobacterium tuberculosis Mycothiol S-transferase enzyme in complex with mycothiol and Zn2+==
		+	<StructureSection load='8f5v' size='340' side='right'caption='[[8f5v]], [[Resolution\|resolution]] 1.45Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[8f5v]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Mycobacterium_tuberculosis Mycobacterium tuberculosis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8F5V OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8F5V FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=XGZ:Mycothiol'>XGZ</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8f5v FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8f5v OCA], [https://pdbe.org/8f5v PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8f5v RCSB], [https://www.ebi.ac.uk/pdbsum/8f5v PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8f5v ProSAT]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/O53728_MYCTU O53728_MYCTU]
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Mycothiol S-transferase (MST) (encoded by the rv0443 gene) was previously identified as the enzyme responsible for the transfer of Mycothiol (MSH) to xenobiotic acceptors in Mycobacterium tuberculosis (M.tb) during xenobiotic stress. To further characterize the functionality of MST in vitro and the possible roles in vivo, X-ray crystallographic, metal-dependent enzyme kinetics, thermal denaturation studies, and antibiotic MIC determination in rv0433 knockout strain were performed. The binding of MSH and Zn(2+) increases the melting temperature by 12.9 degrees C as a consequence of the cooperative stabilization of MST by both MSH and metal. The co-crystal structure of MST in complex with MSH and Zn(2+) to 1.45 A resolution supports the specific utilization of MSH as a substrate as well as affording insights into the structural requirements of MSH binding and the metal-assisted catalytic mechanism of MST. Contrary to the well-defined role of MSH in mycobacterial xenobiotic responses and the ability of MST to bind MSH, cell-based studies with an M.tb rv0443 knockout strain failed to provide evidence for a role of MST in processing of rifampicin or isoniazid. These studies suggest the necessity of a new direction to identify acceptors of the enzyme and better define the biological role of MST in mycobacteria.

-	~~Authors:~~ Jayasinghe, ~~Y.P.~~, ~~Ronning~~, D.R.	+	The Mycobacterium tuberculosis mycothiol S-transferase is divalent metal-dependent for mycothiol binding and transfer.,Jayasinghe YP, Banco MT, Lindenberger JJ, Favrot L, Palcekova Z, Jackson M, Manabe S, Ronning DR RSC Med Chem. 2023 Jan 26;14(3):491-500. doi: 10.1039/d2md00401a. eCollection , 2023 Mar 22. PMID:36970142<ref>PMID:36970142</ref>

-	~~Description: Crystal structure~~ of ~~Mycobacterium tuberculosis Mycothiol~~ S-~~transferase enzyme in complex with mycothiol and Zn2+~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased~~ Structures]]	+	</div>
-	[[Category: Jayasinghe~~, Y.P~~]]	+	<div class="pdbe-citations 8f5v" style="background-color:#fffaf0;"></div>
-	[[Category: Ronning~~, D.R~~]]	+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Mycobacterium tuberculosis]]
		+	[[Category: Jayasinghe YP]]
		+	[[Category: Ronning DR]]

OCA at 04:55, 25 January 2023

OCA — Wed, 25 Jan 2023 04:55:53 GMT

←Older revision		Revision as of 04:55, 25 January 2023
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 8f5v is ON HOLD	+	The entry 8f5v is ON HOLD until Paper Publication

	Authors: Jayasinghe, Y.P., Ronning, D.R.		Authors: Jayasinghe, Y.P., Ronning, D.R.
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	Description: Crystal structure of Mycobacterium tuberculosis Mycothiol S-transferase enzyme in complex with mycothiol and Zn2+		Description: Crystal structure of Mycobacterium tuberculosis Mycothiol S-transferase enzyme in complex with mycothiol and Zn2+
	[[Category: Unreleased Structures]]		[[Category: Unreleased Structures]]
-	[[Category: Ronning, D.R]]
	[[Category: Jayasinghe, Y.P]]		[[Category: Jayasinghe, Y.P]]
		+	[[Category: Ronning, D.R]]

OCA: Protected "8f5v" [edit=sysop:move=sysop]

OCA — Thu, 24 Nov 2022 08:14:15 GMT

Protected "8f5v" [edit=sysop:move=sysop]

←Older revision

Revision as of 08:14, 24 November 2022

OCA: New page: '''Unreleased structure''' The entry 8f5v is ON HOLD Authors: Jayasinghe, Y.P., Ronning, D.R. Description: Crystal structure of Mycobacterium tuberculosis Mycothiol S-transferase enzym...

OCA — Thu, 24 Nov 2022 08:14:13 GMT

New page: '''Unreleased structure''' The entry 8f5v is ON HOLD Authors: Jayasinghe, Y.P., Ronning, D.R. Description: Crystal structure of Mycobacterium tuberculosis Mycothiol S-transferase enzym...

New page

'''Unreleased structure'''

The entry 8f5v is ON HOLD

Authors: Jayasinghe, Y.P., Ronning, D.R.

Description: Crystal structure of Mycobacterium tuberculosis Mycothiol S-transferase enzyme in complex with mycothiol and Zn2+
[[Category: Unreleased Structures]]
[[Category: Ronning, D.R]]
[[Category: Jayasinghe, Y.P]]