OCA at 05:44, 5 July 2023

OCA — Wed, 05 Jul 2023 05:44:51 GMT

←Older revision		Revision as of 05:44, 5 July 2023
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-	'''Unreleased structure'''

-	~~The entry~~ 8fid is ~~ON HOLD until Paper~~ Publication	+	==Crystal Structure of Erwinia tracheiphila CYP114 in complex with ent-kaurenoic acid (Crystal Form 2)==
		+	<StructureSection load='8fid' size='340' side='right'caption='[[8fid]], [[Resolution\|resolution]] 1.83Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[8fid]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Erwinia_tracheiphila Erwinia tracheiphila]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8FID OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8FID FirstGlance]. <br>
		+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.83Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene>, <scene name='pdbligand=NE4:(8alpha,9beta,10alpha,13alpha)-kaur-16-en-18-oic+acid'>NE4</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8fid FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8fid OCA], [https://pdbe.org/8fid PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8fid RCSB], [https://www.ebi.ac.uk/pdbsum/8fid PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8fid ProSAT]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/A0A0M2KDV0_9GAMM A0A0M2KDV0_9GAMM]
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Cytochromes P450 (CYPs) are heme-thiolate monooxygenases that prototypically catalyze the insertion of oxygen into unactivated C-H bonds but are capable of mediating more complex reactions. One of the most remarked-upon alternative reactions occurs during biosynthesis of the gibberellin A (GA) phytohormones, involving hydrocarbon ring contraction with coupled aldehyde extrusion of ent-kaurenoic acid to form the first gibberellin intermediate. While the unusual nature of this reaction has long been noted, its mechanistic basis has remained opaque. Building on identification of the relevant CYP114 from bacterial GA biosynthesis, detailed structure-function studies are reported here, including development of in vitro assays as well as crystallographic analyses both in the absence and presence of substrate. These structures provided insight into enzymatic catalysis of this unusual reaction, as exemplified by identification of a key role for the "missing" acid from an otherwise highly conserved acid-alcohol pair of residues. Notably, the results demonstrate that ring contraction requires dual factors, both the use of a dedicated ferredoxin and absence of the otherwise conserved acidic residue, with exclusion of either limiting turnover to just the initiating and more straightforward hydroxylation. The results provide detailed insight into the enzymatic structure-function relationships underlying this fascinating reaction and support the use of a semipinacol mechanism for the unusual ring contraction reaction.

-	~~Authors~~:	+	Dual factors required for cytochrome-P450-mediated hydrocarbon ring contraction in bacterial gibberellin phytohormone biosynthesis.,Nagel R, Alexander LE, Stewart CE Jr, Peters RJ Proc Natl Acad Sci U S A. 2023 Jun 27;120(26):e2221549120. doi: , 10.1073/pnas.2221549120. Epub 2023 Jun 20. PMID:37339230<ref>PMID:37339230</ref>

-	~~Description~~:	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased~~ Structures]]	+	</div>
		+	<div class="pdbe-citations 8fid" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Erwinia tracheiphila]]
		+	[[Category: Large Structures]]
		+	[[Category: Nagel R]]
		+	[[Category: Stewart Jr CE]]

OCA at 05:05, 18 January 2023

OCA — Wed, 18 Jan 2023 05:05:38 GMT

←Older revision		Revision as of 05:05, 18 January 2023
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 8fid is ON HOLD	+	The entry 8fid is ON HOLD until Paper Publication

	Authors:		Authors:

OCA: Protected "8fid" [edit=sysop:move=sysop]

OCA — Wed, 21 Dec 2022 06:53:28 GMT

Protected "8fid" [edit=sysop:move=sysop]

←Older revision

Revision as of 06:53, 21 December 2022

OCA: New page: '''Unreleased structure''' The entry 8fid is ON HOLD Authors: Description: Category: Unreleased Structures

OCA — Wed, 21 Dec 2022 06:53:23 GMT

New page: '''Unreleased structure''' The entry 8fid is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 8fid is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]

8fid - Revision history

OCA at 05:44, 5 July 2023

OCA at 05:05, 18 January 2023

OCA: Protected "8fid" [edit=sysop:move=sysop]

OCA: New page: '''Unreleased structure''' The entry 8fid is ON HOLD Authors: Description: Category: Unreleased Structures