8fiz - Revision history

OCA at 06:53, 29 March 2023

OCA — Wed, 29 Mar 2023 06:53:07 GMT

←Older revision		Revision as of 06:53, 29 March 2023
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-	'''Unreleased structure'''

-	~~The entry~~ 8fiz is ~~ON HOLD until Paper~~ Publication	+	==Cryo-EM structure of E. coli 70S Ribosome containing mRNA and tRNA (in the transcription-translation complex)==
		+	<StructureSection load='8fiz' size='340' side='right'caption='[[8fiz]], [[Resolution\|resolution]] 3.80Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[8fiz]] is a 10 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8FIZ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8FIZ FirstGlance]. <br>
		+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8fiz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8fiz OCA], [https://pdbe.org/8fiz PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8fiz RCSB], [https://www.ebi.ac.uk/pdbsum/8fiz PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8fiz ProSAT]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	In prokaryotes, translation can occur on mRNA that is being transcribed in a process called coupling. How the ribosome affects the RNA polymerase (RNAP) during coupling is not well understood. Here, we reconstituted the E. coli coupling system and demonstrated that the ribosome can prevent pausing and termination of RNAP and double the overall transcription rate at the expense of fidelity. Moreover, we monitored single RNAPs coupled to ribosomes and show that coupling increases the pause-free velocity of the polymerase and that a mechanical assisting force is sufficient to explain the majority of the effects of coupling. Also, by cryo-EM, we observed that RNAPs with a terminal mismatch adopt a backtracked conformation, while a coupled ribosome allosterically induces these polymerases toward a catalytically active anti-swiveled state. Finally, we demonstrate that prolonged RNAP pausing is detrimental to cell viability, which could be prevented by polymerase reactivation through a coupled ribosome.

-	~~Authors: Florez Ariza,~~ A., Wee~~, L.~~, Tong, A., Canari, C., Grob, P., Nogales, E., Bustamante~~, C~~.	+	A trailing ribosome speeds up RNA polymerase at the expense of transcript fidelity via force and allostery.,Wee LM, Tong AB, Florez Ariza AJ, Canari-Chumpitaz C, Grob P, Nogales E, Bustamante CJ Cell. 2023 Mar 16;186(6):1244-1262.e34. doi: 10.1016/j.cell.2023.02.008. PMID:36931247<ref>PMID:36931247</ref>

-	~~Description: Cryo-EM structure~~ of ~~E. coli 70S Ribosome containing mRNA and tRNA (in~~ the ~~transcription~~-~~translation complex)~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased Structures~~]]	+	</div>
-	[[Category: ~~Nogales, E~~]]	+	<div class="pdbe-citations 8fiz" style="background-color:#fffaf0;"></div>
-	[[Category: ~~Grob, P~~]]	+	== References ==
-	[[Category: ~~Wee, L~~]]	+	<references/>
-	[[Category: ~~Tong,~~ A]]	+	__TOC__
-	[[Category: ~~Canari, C~~]]	+	</StructureSection>
-	[[Category: ~~Florez Ariza,~~ A]]	+	[[Category: Escherichia coli]]
-	[[Category: ~~Bustamante, C~~]]	+	[[Category: Large Structures]]
		+	[[Category: Bustamante C]]
		+	[[Category: Canari C]]
		+	[[Category: Florez Ariza A]]
		+	[[Category: Grob P]]
		+	[[Category: Nogales E]]
		+	[[Category: Tong A]]
		+	[[Category: Wee L]]

OCA at 07:39, 15 March 2023

OCA — Wed, 15 Mar 2023 07:39:53 GMT

←Older revision		Revision as of 07:39, 15 March 2023
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 8fiz is ON HOLD	+	The entry 8fiz is ON HOLD until Paper Publication

	Authors: Florez Ariza, A., Wee, L., Tong, A., Canari, C., Grob, P., Nogales, E., Bustamante, C.		Authors: Florez Ariza, A., Wee, L., Tong, A., Canari, C., Grob, P., Nogales, E., Bustamante, C.
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	[[Category: Grob, P]]		[[Category: Grob, P]]
	[[Category: Wee, L]]		[[Category: Wee, L]]
-	[[Category: Florez Ariza, A]]
-	[[Category: Canari, C]]
	[[Category: Tong, A]]		[[Category: Tong, A]]
		+	[[Category: Canari, C]]
		+	[[Category: Florez Ariza, A]]
	[[Category: Bustamante, C]]		[[Category: Bustamante, C]]

OCA: Protected "8fiz" [edit=sysop:move=sysop]

OCA — Wed, 28 Dec 2022 05:57:26 GMT

Protected "8fiz" [edit=sysop:move=sysop]

←Older revision

Revision as of 05:57, 28 December 2022

OCA: New page: '''Unreleased structure''' The entry 8fiz is ON HOLD Authors: Florez Ariza, A., Wee, L., Tong, A., Canari, C., Grob, P., Nogales, E., Bustamante, C. Description: Cryo-EM structure of E...

OCA — Wed, 28 Dec 2022 05:57:23 GMT

New page: '''Unreleased structure''' The entry 8fiz is ON HOLD Authors: Florez Ariza, A., Wee, L., Tong, A., Canari, C., Grob, P., Nogales, E., Bustamante, C. Description: Cryo-EM structure of E...

New page

'''Unreleased structure'''

The entry 8fiz is ON HOLD

Authors: Florez Ariza, A., Wee, L., Tong, A., Canari, C., Grob, P., Nogales, E., Bustamante, C.

Description: Cryo-EM structure of E. coli 70S Ribosome containing mRNA and tRNA (in the transcription-translation complex)
[[Category: Unreleased Structures]]
[[Category: Nogales, E]]
[[Category: Grob, P]]
[[Category: Wee, L]]
[[Category: Florez Ariza, A]]
[[Category: Canari, C]]
[[Category: Tong, A]]
[[Category: Bustamante, C]]