8flo - Revision history

OCA at 10:48, 22 May 2024

OCA — Wed, 22 May 2024 10:48:07 GMT

←Older revision		Revision as of 10:48, 22 May 2024
Line 4:		Line 4:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[8flo]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Mycobacterium_marinum_ATCC_BAA-535 Mycobacterium marinum ATCC BAA-535]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8FLO OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8FLO FirstGlance]. <br>		<table><tr><td colspan='2'>[[8flo]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Mycobacterium_marinum_ATCC_BAA-535 Mycobacterium marinum ATCC BAA-535]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8FLO OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8FLO FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.71Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8flo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8flo OCA], [https://pdbe.org/8flo PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8flo RCSB], [https://www.ebi.ac.uk/pdbsum/8flo PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8flo ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8flo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8flo OCA], [https://pdbe.org/8flo PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8flo RCSB], [https://www.ebi.ac.uk/pdbsum/8flo PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8flo ProSAT]</span></td></tr>
	</table>		</table>

OCA at 06:53, 29 March 2023

OCA — Wed, 29 Mar 2023 06:53:48 GMT

←Older revision		Revision as of 06:53, 29 March 2023
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 8flo is ~~ON HOLD until Paper~~ Publication	+	==X-ray crystal structure of substrate free CYP124A1 from Mycobacterium Marinum==
		+	<StructureSection load='8flo' size='340' side='right'caption='[[8flo]], [[Resolution\|resolution]] 1.71Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[8flo]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Mycobacterium_marinum_ATCC_BAA-535 Mycobacterium marinum ATCC BAA-535]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8FLO OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8FLO FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8flo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8flo OCA], [https://pdbe.org/8flo PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8flo RCSB], [https://www.ebi.ac.uk/pdbsum/8flo PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8flo ProSAT]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The CYP124 family of cytochrome P450 enzymes, as exemplified by CYP124A1 from Mycobacterium tuberculosis, is involved in the metabolism of methyl branched lipids and cholesterol derivatives. The equivalent enzyme from Mycobacterium marinum was investigated to compare the degree of functional conservation between members of this CYP family from closely related bacteria. We compared substrate binding of each CYP124 enzyme using UV-vis spectroscopy and the catalytic oxidation of methyl branched lipids, terpenes and cholesterol derivatives was investigated. The CYP124 enzyme from M. tuberculosis displayed a larger shift to the ferric high-spin state on binding cholesterol derivatives compared to the equivalent enzyme from M. marinum. The biggest difference was observed with cholesteryl sulfate which induced distinct UV-vis spectra in each CYP124 enzyme. The selectivity for oxidation at the omega-carbon of a branched chain was maintained for all substrates, except cholesteryl sulfate which was not oxidized by either enzyme. The CYP124A1 enzyme from M. marinum, in combination with farnesol and farnesyl acetate, was structurally characterized by X-ray crystallography. These ligand-bound structures of the CYP124 enzyme revealed that the polar component of the substrates bound in a different manner to that of phytanic acid in the structure of CYP124A1 from M. tuberculosis. However, closer to the heme the structures were similar providing an explanation for the high selectivity of the enzyme for terminal methyl C-H bond oxidation. The work here demonstrates that there were differences in the biochemistry of the CYP124 enzymes from these closely related bacteria.

-	~~Authors: Ghith~~, A., Bruning, J.B.~~, Bell, S~~.G.	+	The catalytic activity and structure of the lipid metabolizing CYP124 cytochrome P450 enzyme from Mycobacterium marinum.,Ghith A, Bruning JB, Bell SG Arch Biochem Biophys. 2023 Mar 15;737:109554. doi: 10.1016/j.abb.2023.109554. , Epub 2023 Feb 25. PMID:36842492<ref>PMID:36842492</ref>

-	~~Description: X-ray crystal structure~~ of ~~substrate free CYP124A1 from Mycobacterium Marinum~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased~~ Structures]]	+	</div>
-	[[Category: ~~Bruning, J.B~~]]	+	<div class="pdbe-citations 8flo" style="background-color:#fffaf0;"></div>
-	[[Category: ~~Ghith, A~~]]	+	== References ==
-	[[Category: ~~Bell, S.G~~]]	+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Mycobacterium marinum ATCC BAA-535]]
		+	[[Category: Bell SG]]
		+	[[Category: Bruning JB]]
		+	[[Category: Ghith A]]

OCA at 08:41, 15 February 2023

OCA — Wed, 15 Feb 2023 08:41:28 GMT

←Older revision		Revision as of 08:41, 15 February 2023
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 8flo is ON HOLD	+	The entry 8flo is ON HOLD until Paper Publication

	Authors: Ghith, A., Bruning, J.B., Bell, S.G.		Authors: Ghith, A., Bruning, J.B., Bell, S.G.

OCA: Protected "8flo" [edit=sysop:move=sysop]

OCA — Wed, 28 Dec 2022 06:12:20 GMT

Protected "8flo" [edit=sysop:move=sysop]

←Older revision

Revision as of 06:12, 28 December 2022

OCA: New page: '''Unreleased structure''' The entry 8flo is ON HOLD Authors: Ghith, A., Bruning, J.B., Bell, S.G. Description: X-ray crystal structure of substrate free CYP124A1 from Mycobacterium Ma...

OCA — Wed, 28 Dec 2022 06:12:15 GMT

New page: '''Unreleased structure''' The entry 8flo is ON HOLD Authors: Ghith, A., Bruning, J.B., Bell, S.G. Description: X-ray crystal structure of substrate free CYP124A1 from Mycobacterium Ma...

New page

'''Unreleased structure'''

The entry 8flo is ON HOLD

Authors: Ghith, A., Bruning, J.B., Bell, S.G.

Description: X-ray crystal structure of substrate free CYP124A1 from Mycobacterium Marinum
[[Category: Unreleased Structures]]
[[Category: Bruning, J.B]]
[[Category: Ghith, A]]
[[Category: Bell, S.G]]