OCA at 10:04, 17 January 2024

OCA — Wed, 17 Jan 2024 10:04:42 GMT

←Older revision		Revision as of 10:04, 17 January 2024
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-	'''Unreleased structure'''

-	~~The entry~~ 8ftl is ~~ON HOLD until Paper Publication~~	+	==Crystal structure of the SARS-CoV-2 (COVID-19) main protease (Mpro) in complex with inhibitor Jun89-3-C1==
-		+	<StructureSection load='8ftl' size='340' side='right'caption='[[8ftl]], [[Resolution\|resolution]] 2.08Å' scene=''>
-	~~Authors~~: ~~Lewandowski~~, E.M., ~~Butler, S~~.G., Hu, Y., ~~Tan~~, H., ~~Wang~~, J., ~~Chen~~, Y.	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>[[8ftl]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Severe_acute_respiratory_syndrome_coronavirus_2 Severe acute respiratory syndrome coronavirus 2]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8FTL OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8FTL FirstGlance]. <br>
-	~~Description~~: ~~Crystal structure~~ of the ~~SARS~~-~~CoV~~-2 (~~COVID~~-19) ~~main protease~~ (~~Mpro~~) in ~~complex~~ with ~~inhibitor Jun89~~-3-C1	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.08Å</td></tr>
-	[[Category: ~~Unreleased~~ Structures]]	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=5ZF:N-([1,1-biphenyl]-4-yl)-2-chloro-N-[(1R)-2-oxo-2-{[(1S)-1-phenylethyl]amino}-1-(pyridin-3-yl)ethyl]acetamide'>5ZF</scene></td></tr>
-	[[Category: ~~Hu, Y~~]]	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8ftl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8ftl OCA], [https://pdbe.org/8ftl PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8ftl RCSB], [https://www.ebi.ac.uk/pdbsum/8ftl PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8ftl ProSAT]</span></td></tr>
-	[[Category: ~~Lewandowski, E.M~~]]	+	</table>
-	[[Category: ~~Tan, H~~]]	+	== Function ==
-	[[Category: ~~Chen,~~ Y]]	+	[https://www.uniprot.org/uniprot/R1AB_SARS2 R1AB_SARS2] Multifunctional protein involved in the transcription and replication of viral RNAs. Contains the proteinases responsible for the cleavages of the polyprotein.[UniProtKB:P0C6X7] Inhibits host translation by interacting with the 40S ribosomal subunit. The nsp1-40S ribosome complex further induces an endonucleolytic cleavage near the 5'UTR of host mRNAs, targeting them for degradation. Viral mRNAs are not susceptible to nsp1-mediated endonucleolytic RNA cleavage thanks to the presence of a 5'-end leader sequence and are therefore protected from degradation. By suppressing host gene expression, nsp1 facilitates efficient viral gene expression in infected cells and evasion from host immune response.[UniProtKB:P0C6X7] May play a role in the modulation of host cell survival signaling pathway by interacting with host PHB and PHB2. Indeed, these two proteins play a role in maintaining the functional integrity of the mitochondria and protecting cells from various stresses.[UniProtKB:P0C6X7] Responsible for the cleavages located at the N-terminus of the replicase polyprotein. In addition, PL-PRO possesses a deubiquitinating/deISGylating activity and processes both 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains from cellular substrates. Participates together with nsp4 in the assembly of virally-induced cytoplasmic double-membrane vesicles necessary for viral replication. Antagonizes innate immune induction of type I interferon by blocking the phosphorylation, dimerization and subsequent nuclear translocation of host IRF3. Prevents also host NF-kappa-B signaling.[UniProtKB:P0C6X7] Participates in the assembly of virally-induced cytoplasmic double-membrane vesicles necessary for viral replication.[UniProtKB:P0C6X7] Cleaves the C-terminus of replicase polyprotein at 11 sites. Recognizes substrates containing the core sequence [ILMVF]-Q-\|-[SGACN] (PubMed:32198291). Also able to bind an ADP-ribose-1''-phosphate (ADRP).[UniProtKB:P0C6X7]<ref>PMID:32198291</ref> Plays a role in the initial induction of autophagosomes from host reticulum endoplasmic. Later, limits the expansion of these phagosomes that are no longer able to deliver viral components to lysosomes.[UniProtKB:P0C6X7] Forms a hexadecamer with nsp8 (8 subunits of each) that may participate in viral replication by acting as a primase. Alternatively, may synthesize substantially longer products than oligonucleotide primers.[UniProtKB:P0C6X7] Forms a hexadecamer with nsp7 (8 subunits of each) that may participate in viral replication by acting as a primase. Alternatively, may synthesize substantially longer products than oligonucleotide primers.[UniProtKB:P0C6X7] May participate in viral replication by acting as a ssRNA-binding protein.[UniProtKB:P0C6X7] Plays a pivotal role in viral transcription by stimulating both nsp14 3'-5' exoribonuclease and nsp16 2'-O-methyltransferase activities. Therefore plays an essential role in viral mRNAs cap methylation.[UniProtKB:P0C6X7] Responsible for replication and transcription of the viral RNA genome.[UniProtKB:P0C6X7] Multi-functional protein with a zinc-binding domain in N-terminus displaying RNA and DNA duplex-unwinding activities with 5' to 3' polarity. Activity of helicase is dependent on magnesium.[UniProtKB:P0C6X7] Enzyme possessing two different activities: an exoribonuclease activity acting on both ssRNA and dsRNA in a 3' to 5' direction and a N7-guanine methyltransferase activity. Acts as a proofreading exoribonuclease for RNA replication, thereby lowering The sensitivity of the virus to RNA mutagens.[UniProtKB:P0C6X7] Mn(2+)-dependent, uridylate-specific enzyme, which leaves 2'-3'-cyclic phosphates 5' to the cleaved bond.[UniProtKB:P0C6X7] Methyltransferase that mediates mRNA cap 2'-O-ribose methylation to the 5'-cap structure of viral mRNAs. N7-methyl guanosine cap is a prerequisite for binding of nsp16. Therefore plays an essential role in viral mRNAs cap methylation which is essential to evade immune system.[UniProtKB:P0C6X7]
-	[[Category: ~~Wang, J~~]]	+	== References ==
-	[[Category: ~~Butler, S.G~~]]	+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Severe acute respiratory syndrome coronavirus 2]]
		+	[[Category: Butler SG]]
		+	[[Category: Chen Y]]
		+	[[Category: Hu Y]]
		+	[[Category: Lewandowski EM]]
		+	[[Category: Tan H]]
		+	[[Category: Wang J]]

OCA at 10:01, 3 January 2024

OCA — Wed, 03 Jan 2024 10:01:46 GMT

←Older revision		Revision as of 10:01, 3 January 2024
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 8ftl is ON HOLD	+	The entry 8ftl is ON HOLD until Paper Publication

	Authors: Lewandowski, E.M., Butler, S.G., Hu, Y., Tan, H., Wang, J., Chen, Y.		Authors: Lewandowski, E.M., Butler, S.G., Hu, Y., Tan, H., Wang, J., Chen, Y.
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	Description: Crystal structure of the SARS-CoV-2 (COVID-19) main protease (Mpro) in complex with inhibitor Jun89-3-C1		Description: Crystal structure of the SARS-CoV-2 (COVID-19) main protease (Mpro) in complex with inhibitor Jun89-3-C1
	[[Category: Unreleased Structures]]		[[Category: Unreleased Structures]]
		+	[[Category: Hu, Y]]
	[[Category: Lewandowski, E.M]]		[[Category: Lewandowski, E.M]]
	[[Category: Tan, H]]		[[Category: Tan, H]]
-	[[Category: Butler, S.G]]
-	[[Category: Hu, Y]]
	[[Category: Chen, Y]]		[[Category: Chen, Y]]
	[[Category: Wang, J]]		[[Category: Wang, J]]
		+	[[Category: Butler, S.G]]

OCA: Protected "8ftl" [edit=sysop:move=sysop]

OCA — Wed, 18 Jan 2023 05:16:36 GMT

Protected "8ftl" [edit=sysop:move=sysop]

←Older revision

Revision as of 05:16, 18 January 2023

OCA: New page: '''Unreleased structure''' The entry 8ftl is ON HOLD Authors: Lewandowski, E.M., Butler, S.G., Hu, Y., Tan, H., Wang, J., Chen, Y. Description: Crystal structure of the SARS-CoV-2 (COV...

OCA — Wed, 18 Jan 2023 05:16:35 GMT

New page: '''Unreleased structure''' The entry 8ftl is ON HOLD Authors: Lewandowski, E.M., Butler, S.G., Hu, Y., Tan, H., Wang, J., Chen, Y. Description: Crystal structure of the SARS-CoV-2 (COV...

New page

'''Unreleased structure'''

The entry 8ftl is ON HOLD

Authors: Lewandowski, E.M., Butler, S.G., Hu, Y., Tan, H., Wang, J., Chen, Y.

Description: Crystal structure of the SARS-CoV-2 (COVID-19) main protease (Mpro) in complex with inhibitor Jun89-3-C1
[[Category: Unreleased Structures]]
[[Category: Lewandowski, E.M]]
[[Category: Tan, H]]
[[Category: Butler, S.G]]
[[Category: Hu, Y]]
[[Category: Chen, Y]]
[[Category: Wang, J]]

8ftl - Revision history

OCA at 10:04, 17 January 2024

OCA at 10:01, 3 January 2024

OCA: Protected "8ftl" [edit=sysop:move=sysop]

OCA: New page: '''Unreleased structure''' The entry 8ftl is ON HOLD Authors: Lewandowski, E.M., Butler, S.G., Hu, Y., Tan, H., Wang, J., Chen, Y. Description: Crystal structure of the SARS-CoV-2 (COV...