OCA at 12:38, 26 July 2023

OCA — Wed, 26 Jul 2023 12:38:15 GMT

←Older revision		Revision as of 12:38, 26 July 2023
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-	'''Unreleased structure'''

-	~~The entry~~ 8fw5 is ~~ON HOLD~~ ~~until Paper~~ Publication	+	==Chimeric HsGATOR1-SpGtr-SpLam complex==
		+	<StructureSection load='8fw5' size='340' side='right'caption='[[8fw5]], [[Resolution\|resolution]] 3.08Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[8fw5]] is a 9 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] and [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8FW5 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8FW5 FirstGlance]. <br>
		+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution\|Resolution]] 3.08Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=AF3:ALUMINUM+FLUORIDE'>AF3</scene>, <scene name='pdbligand=GDP:GUANOSINE-5-DIPHOSPHATE'>GDP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8fw5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8fw5 OCA], [https://pdbe.org/8fw5 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8fw5 RCSB], [https://www.ebi.ac.uk/pdbsum/8fw5 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8fw5 ProSAT]</span></td></tr>
		+	</table>
		+	== Disease ==
		+	[https://www.uniprot.org/uniprot/DEPD5_HUMAN DEPD5_HUMAN] Rolandic epilepsy;Autosomal dominant nocturnal frontal lobe epilepsy;Autosomal dominant epilepsy with auditory features;Familial focal epilepsy with variable foci. The disease is caused by mutations affecting the gene represented in this entry. Inactivating mutations and truncating deletions in the genes encoding GATOR1 proteins, including DEPDC5, are detected in glioblastoma and ovarian tumors and are associated with loss of heterozygosity events. Inactivation of GATOR1 proteins promotes constitutive localization of mTORC1 to the lysosomal membrane and blocks mTORC1 inactivation following amino acid withdrawal (PubMed:23723238).<ref>PMID:23723238</ref>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/DEPD5_HUMAN DEPD5_HUMAN] As a component of the GATOR1 complex functions as an inhibitor of the amino acid-sensing branch of the TORC1 pathway. The GATOR1 complex strongly increases GTP hydrolysis by RRAGA and RRAGB within RRAGC-containing heterodimers, thereby deactivating RRAGs, releasing mTORC1 from lysosomal surface and inhibiting mTORC1 signaling. The GATOR1 complex is negatively regulated by GATOR2 the other GATOR subcomplex in this amino acid-sensing branch of the TORC1 pathway.<ref>PMID:23723238</ref> <ref>PMID:25457612</ref>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	mTORC1 is a protein kinase complex that controls cellular growth in response to nutrient availability. Amino acid signals are transmitted toward mTORC1 via the Rag/Gtr GTPases and their upstream regulators. An important regulator is LAMTOR, which localizes Rag/Gtr on the lysosomal/vacuole membrane. In human cells, LAMTOR consists of five subunits, but in yeast, only three or four. Currently, it is not known how variation of the subunit stoichiometry may affect its structural organization and biochemical properties. Here, we report a 3.1 A-resolution structural model of the Gtr-Lam complex in Schizosaccharomyces pombe. We found that SpGtr shares conserved architecture as HsRag, but the intersubunit communication that coordinates nucleotide loading on the two subunits differs. In contrast, SpLam contains distinctive structural features, but its GTP-specific GEF activity toward SpGtr is evolutionarily conserved. Our results revealed unique evolutionary paths of the protein components of the mTORC1 pathway.

-	~~Authors~~:	+	Structure of the Schizosaccharomyces pombe Gtr-Lam complex reveals evolutionary divergence of mTORC1-dependent amino acid sensing.,Tettoni SD, Egri SB, Doxsey DD, Veinotte K, Ouch C, Chang JY, Song K, Xu C, Shen K Structure. 2023 Jul 4:S0969-2126(23)00208-3. doi: 10.1016/j.str.2023.06.012. PMID:37453417<ref>PMID:37453417</ref>

-	~~Description~~:	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased~~ Structures]]	+	</div>
		+	<div class="pdbe-citations 8fw5" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Escherichia coli]]
		+	[[Category: Homo sapiens]]
		+	[[Category: Large Structures]]
		+	[[Category: Chang J]]
		+	[[Category: Doxsey DD]]
		+	[[Category: Egri SB]]
		+	[[Category: Ouch C]]
		+	[[Category: Shen K]]
		+	[[Category: Song K]]
		+	[[Category: Tettoni SD]]
		+	[[Category: Xu C]]

OCA at 04:25, 2 March 2023

OCA — Thu, 02 Mar 2023 04:25:21 GMT

←Older revision		Revision as of 04:25, 2 March 2023
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 8fw5 is ON HOLD	+	The entry 8fw5 is ON HOLD until Paper Publication

	Authors:		Authors:

OCA: Protected "8fw5" [edit=sysop:move=sysop]

OCA — Wed, 01 Feb 2023 09:24:44 GMT

Protected "8fw5" [edit=sysop:move=sysop]

←Older revision

Revision as of 09:24, 1 February 2023

OCA: New page: '''Unreleased structure''' The entry 8fw5 is ON HOLD Authors: Description: Category: Unreleased Structures

OCA — Wed, 01 Feb 2023 09:24:43 GMT

New page: '''Unreleased structure''' The entry 8fw5 is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 8fw5 is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]

8fw5 - Revision history

OCA at 12:38, 26 July 2023

OCA at 04:25, 2 March 2023

OCA: Protected "8fw5" [edit=sysop:move=sysop]

OCA: New page: '''Unreleased structure''' The entry 8fw5 is ON HOLD Authors: Description: Category: Unreleased Structures