OCA at 14:05, 18 October 2023

OCA — Wed, 18 Oct 2023 14:05:53 GMT

←Older revision		Revision as of 14:05, 18 October 2023
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-	'''Unreleased structure'''

-	~~The entry~~ 8fzo is ~~ON HOLD until Paper~~ Publication	+	==Crystal structure of polyreactive 338E6 mouse Fab==
		+	<StructureSection load='8fzo' size='340' side='right'caption='[[8fzo]], [[Resolution\|resolution]] 1.83Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[8fzo]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8FZO OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8FZO FirstGlance]. <br>
		+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.83Å</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8fzo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8fzo OCA], [https://pdbe.org/8fzo PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8fzo RCSB], [https://www.ebi.ac.uk/pdbsum/8fzo PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8fzo ProSAT]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	To become specialized binders, antibodies undergo a process called affinity maturation to maximize their binding affinity. Despite this process, some antibodies retain low-affinity binding to diverse epitopes in a phenomenon called polyreactivity. Here we seek to understand the molecular basis of this polyreactivity in antibodies. Our results highlight that polyreactive antigen-binding fragments (Fabs) bind their targets with low affinities, comparable to T cell receptor recognition of autologous classical major histocompatibility complex. Extensive mutagenic studies find no singular amino acid residue or biochemical property responsible for polyreactive interaction, suggesting that polyreactive antibodies use multiple strategies for engagement. Finally, our crystal structures and all-atom molecular dynamics simulations of polyreactive Fabs show increased rigidity compared to their monoreactive relatives, forming a neutral and accessible platform for diverse antigens to bind. Together, these data support a cooperative strategy of rigid neutrality in establishing the polyreactive status of an antibody molecule.

-	~~Authors:~~ Borowska, ~~M.T.~~, ~~Adams~~, E.J.	+	Biochemical and biophysical characterization of natural polyreactivity in antibodies.,Borowska MT, Boughter CT, Bunker JJ, Guthmiller JJ, Wilson PC, Roux B, Bendelac A, Adams EJ Cell Rep. 2023 Oct 5;42(10):113190. doi: 10.1016/j.celrep.2023.113190. PMID:37804505<ref>PMID:37804505</ref>

-	~~Description: Crystal structure~~ of ~~polyreactive 338E6 mouse Fab~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased~~ Structures]]	+	</div>
-	[[Category: ~~Borowska, M.T~~]]	+	<div class="pdbe-citations 8fzo" style="background-color:#fffaf0;"></div>
-	[[Category: Adams~~, E.J~~]]	+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Mus musculus]]
		+	[[Category: Adams EJ]]
		+	[[Category: Borowska MT]]

OCA at 05:43, 8 March 2023

OCA — Wed, 08 Mar 2023 05:43:29 GMT

←Older revision		Revision as of 05:43, 8 March 2023
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 8fzo is ON HOLD	+	The entry 8fzo is ON HOLD until Paper Publication

	Authors: Borowska, M.T., Adams, E.J.		Authors: Borowska, M.T., Adams, E.J.

OCA: Protected "8fzo" [edit=sysop:move=sysop]

OCA — Wed, 08 Feb 2023 05:13:42 GMT

Protected "8fzo" [edit=sysop:move=sysop]

←Older revision

Revision as of 05:13, 8 February 2023

OCA: New page: '''Unreleased structure''' The entry 8fzo is ON HOLD Authors: Borowska, M.T., Adams, E.J. Description: Crystal structure of polyreactive 338E6 mouse Fab [[Category: Unreleased Structur...

OCA — Wed, 08 Feb 2023 05:13:41 GMT

New page: '''Unreleased structure''' The entry 8fzo is ON HOLD Authors: Borowska, M.T., Adams, E.J. Description: Crystal structure of polyreactive 338E6 mouse Fab [[Category: Unreleased Structur...

New page

'''Unreleased structure'''

The entry 8fzo is ON HOLD

Authors: Borowska, M.T., Adams, E.J.

Description: Crystal structure of polyreactive 338E6 mouse Fab
[[Category: Unreleased Structures]]
[[Category: Borowska, M.T]]
[[Category: Adams, E.J]]

8fzo - Revision history

OCA at 14:05, 18 October 2023

OCA at 05:43, 8 March 2023

OCA: Protected "8fzo" [edit=sysop:move=sysop]

OCA: New page: '''Unreleased structure''' The entry 8fzo is ON HOLD Authors: Borowska, M.T., Adams, E.J. Description: Crystal structure of polyreactive 338E6 mouse Fab [[Category: Unreleased Structur...