OCA at 14:05, 18 October 2023

OCA — Wed, 18 Oct 2023 14:05:58 GMT

←Older revision		Revision as of 14:05, 18 October 2023
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 8fzw is ~~ON HOLD until Paper~~ Publication	+	==Thaumatin crystallized in cyclic olefin copolymer-based microfluidic chips==
		+	<StructureSection load='8fzw' size='340' side='right'caption='[[8fzw]], [[Resolution\|resolution]] 1.48Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[8fzw]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Thaumatococcus_daniellii Thaumatococcus daniellii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8FZW OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8FZW FirstGlance]. <br>
		+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.48Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=TLA:L(+)-TARTARIC+ACID'>TLA</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8fzw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8fzw OCA], [https://pdbe.org/8fzw PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8fzw RCSB], [https://www.ebi.ac.uk/pdbsum/8fzw PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8fzw ProSAT]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/THM1_THADA THM1_THADA] Taste-modifying protein; intensely sweet-tasting. It is 100000 times sweeter than sucrose on a molar basis.
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Over the past two decades, serial X-ray crystallography has enabled the structure determination of a wide range of proteins. With the advent of X-ray free-electron lasers (XFELs), ever-smaller crystals have yielded high-resolution diffraction and structure determination. A crucial need to continue advancement is the efficient delivery of fragile and micrometre-sized crystals to the X-ray beam intersection. This paper presents an improved design of an all-polymer microfluidic ;chip' for room-temperature fixed-target serial crystallography that can be tailored to broadly meet the needs of users at either synchrotron or XFEL light sources. The chips are designed to be customized around different types of crystals and offer users a friendly, quick, convenient, ultra-low-cost and robust sample-delivery platform. Compared with the previous iteration of the chip [Gilbile et al. (2021), Lab Chip, 21, 4831-4845], the new design eliminates cleanroom fabrication. It has a larger imaging area to volume, while maintaining crystal hydration stability for both in situ crystallization or direct crystal slurry loading. Crystals of two model proteins, lysozyme and thaumatin, were used to validate the effectiveness of the design at both synchrotron (lysozyme and thaumatin) and XFEL (lysozyme only) facilities, yielding complete data sets with resolutions of 1.42, 1.48 and 1.70 A, respectively. Overall, the improved chip design, ease of fabrication and high modifiability create a powerful, all-around sample-delivery tool that structural biologists can quickly adopt, especially in cases of limited sample volume and small, fragile crystals.

-	~~Authors: Liu~~, Z., Gu~~, K.~~, Shelby~~, M.L.~~, Gilbile, D., Lyubimov~~, A.Y.~~, Russi, S., Cohen, A~~.E.~~, Coleman~~, M.A.~~, Frank, M., Kuhl, T.L.	+	A user-friendly plug-and-play cyclic olefin copolymer-based microfluidic chip for room-temperature, fixed-target serial crystallography.,Liu Z, Gu KK, Shelby ML, Gilbile D, Lyubimov AY, Russi S, Cohen AE, Narayanasamy SR, Botha S, Kupitz C, Sierra RG, Poitevin F, Gilardi A, Lisova S, Coleman MA, Frank M, Kuhl TL Acta Crystallogr D Struct Biol. 2023 Oct 1;79(Pt 10):944-952. doi: , 10.1107/S2059798323007027. Epub 2023 Sep 25. PMID:37747292<ref>PMID:37747292</ref>

-	~~Description: Thaumatin crystallized in cyclic olefin copolymer~~-~~based microfluidic chips~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased~~ Structures]]	+	</div>
-	[[Category: ~~Cohen, A.E~~]]	+	<div class="pdbe-citations 8fzw" style="background-color:#fffaf0;"></div>
-	[[Category: ~~Kuhl, T.L~~]]	+	== References ==
-	[[Category: ~~Russi, S~~]]	+	<references/>
-	[[Category: ~~Lyubimov, A.Y~~]]	+	__TOC__
-	[[Category: ~~Frank, M~~]]	+	</StructureSection>
-	[[Category: ~~Coleman, M.A~~]]	+	[[Category: Large Structures]]
-	[[Category: ~~Gilbile, D~~]]	+	[[Category: Thaumatococcus daniellii]]
-	[[Category: Liu, Z]]	+	[[Category: Cohen AE]]
-	[[Category: ~~Shelby, M.L~~]]	+	[[Category: Coleman MA]]
-	[[Category: ~~Gu, K~~]]	+	[[Category: Frank M]]
		+	[[Category: Gilbile D]]
		+	[[Category: Gu K]]
		+	[[Category: Kuhl TL]]
		+	[[Category: Liu Z]]
		+	[[Category: Lyubimov AY]]
		+	[[Category: Russi S]]
		+	[[Category: Shelby ML]]

OCA at 04:53, 7 April 2023

OCA — Fri, 07 Apr 2023 04:53:07 GMT

←Older revision		Revision as of 04:53, 7 April 2023
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 8fzw is ON HOLD	+	The entry 8fzw is ON HOLD until Paper Publication

-	Authors: Liu, Z., Kuhl, T.L.	+	Authors: Liu, Z., Gu, K., Shelby, M.L., Gilbile, D., Lyubimov, A.Y., Russi, S., Cohen, A.E., Coleman, M.A., Frank, M., Kuhl, T.L.

	Description: Thaumatin crystallized in cyclic olefin copolymer-based microfluidic chips		Description: Thaumatin crystallized in cyclic olefin copolymer-based microfluidic chips
	[[Category: Unreleased Structures]]		[[Category: Unreleased Structures]]
		+	[[Category: Cohen, A.E]]
	[[Category: Kuhl, T.L]]		[[Category: Kuhl, T.L]]
		+	[[Category: Russi, S]]
		+	[[Category: Lyubimov, A.Y]]
		+	[[Category: Frank, M]]
		+	[[Category: Coleman, M.A]]
		+	[[Category: Gilbile, D]]
	[[Category: Liu, Z]]		[[Category: Liu, Z]]
		+	[[Category: Shelby, M.L]]
		+	[[Category: Gu, K]]

OCA: Protected "8fzw" [edit=sysop:move=sysop]

OCA — Wed, 08 Feb 2023 05:14:16 GMT

Protected "8fzw" [edit=sysop:move=sysop]

←Older revision

Revision as of 05:14, 8 February 2023

OCA: New page: '''Unreleased structure''' The entry 8fzw is ON HOLD Authors: Liu, Z., Kuhl, T.L. Description: Thaumatin crystallized in cyclic olefin copolymer-based microfluidic chips [[Category: Un...

OCA — Wed, 08 Feb 2023 05:14:15 GMT

New page: '''Unreleased structure''' The entry 8fzw is ON HOLD Authors: Liu, Z., Kuhl, T.L. Description: Thaumatin crystallized in cyclic olefin copolymer-based microfluidic chips [[Category: Un...

New page

'''Unreleased structure'''

The entry 8fzw is ON HOLD

Authors: Liu, Z., Kuhl, T.L.

Description: Thaumatin crystallized in cyclic olefin copolymer-based microfluidic chips
[[Category: Unreleased Structures]]
[[Category: Kuhl, T.L]]
[[Category: Liu, Z]]

8fzw - Revision history

OCA at 14:05, 18 October 2023

OCA at 04:53, 7 April 2023

OCA: Protected "8fzw" [edit=sysop:move=sysop]

OCA: New page: '''Unreleased structure''' The entry 8fzw is ON HOLD Authors: Liu, Z., Kuhl, T.L. Description: Thaumatin crystallized in cyclic olefin copolymer-based microfluidic chips [[Category: Un...