OCA at 12:38, 26 July 2023

OCA — Wed, 26 Jul 2023 12:38:21 GMT

←Older revision		Revision as of 12:38, 26 July 2023
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-	'''Unreleased structure'''

-	~~The entry~~ 8g01 is ~~ON HOLD until Paper~~ Publication	+	==YES Complex - E. coli MraY, Protein E ID21, E. coli SlyD==
		+	<StructureSection load='8g01' size='340' side='right'caption='[[8g01]], [[Resolution\|resolution]] 3.40Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[8g01]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_K-12 Escherichia coli K-12] and [https://en.wikipedia.org/wiki/Escherichia_phage_ID21 Escherichia phage ID21]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8G01 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8G01 FirstGlance]. <br>
		+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution\|Resolution]] 3.4Å</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8g01 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8g01 OCA], [https://pdbe.org/8g01 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8g01 RCSB], [https://www.ebi.ac.uk/pdbsum/8g01 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8g01 ProSAT]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/MRAY_ECOLI MRAY_ECOLI] Catalyzes the initial step of the lipid cycle reactions in the biosynthesis of the cell wall peptidoglycan: transfers peptidoglycan precursor phospho-MurNAc-pentapeptide from UDP-MurNAc-pentapeptide onto the lipid carrier undecaprenyl phosphate, yielding undecaprenyl-pyrophosphoryl-MurNAc-pentapeptide, known as lipid I.[HAMAP-Rule:MF_00038]<ref>PMID:1846850</ref> <ref>PMID:215212</ref>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The historically important phage PhiX174 kills its host bacteria by encoding a 91-residue protein antibiotic called protein E. Using single-particle electron cryo-microscopy, we demonstrate that protein E bridges two bacterial proteins to form the transmembrane YES complex [MraY, protein E, sensitivity to lysis D (SlyD)]. Protein E inhibits peptidoglycan biosynthesis by obstructing the MraY active site leading to loss of lipid I production. We experimentally validate this result for two different viral species, providing a clear model for bacterial lysis and unifying previous experimental data. Additionally, we characterize the Escherichia coli MraY structure-revealing features of this essential enzyme-and the structure of the chaperone SlyD bound to a protein. Our structures provide insights into the mechanism of phage-mediated lysis and for structure-based design of phage therapeutics.

-	~~Authors~~:	+	The mechanism of the phage-encoded protein antibiotic from PhiX174.,Orta AK, Riera N, Li YE, Tanaka S, Yun HG, Klaic L, Clemons WM Jr Science. 2023 Jul 14;381(6654):eadg9091. doi: 10.1126/science.adg9091. Epub 2023 , Jul 14. PMID:37440661<ref>PMID:37440661</ref>

-	~~Description~~:	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased~~ Structures]]	+	</div>
		+	<div class="pdbe-citations 8g01" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Escherichia coli K-12]]
		+	[[Category: Escherichia phage ID21]]
		+	[[Category: Large Structures]]
		+	[[Category: Clemons WM]]
		+	[[Category: Orta AK]]
		+	[[Category: Riera N]]

OCA at 05:43, 8 March 2023

OCA — Wed, 08 Mar 2023 05:43:44 GMT

←Older revision		Revision as of 05:43, 8 March 2023
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 8g01 is ON HOLD	+	The entry 8g01 is ON HOLD until Paper Publication

	Authors:		Authors:

OCA: Protected "8g01" [edit=sysop:move=sysop]

OCA — Wed, 08 Feb 2023 05:14:33 GMT

Protected "8g01" [edit=sysop:move=sysop]

←Older revision

Revision as of 05:14, 8 February 2023

OCA: New page: '''Unreleased structure''' The entry 8g01 is ON HOLD Authors: Description: Category: Unreleased Structures

OCA — Wed, 08 Feb 2023 05:14:32 GMT

New page: '''Unreleased structure''' The entry 8g01 is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 8g01 is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]

8g01 - Revision history

OCA at 12:38, 26 July 2023

OCA at 05:43, 8 March 2023

OCA: Protected "8g01" [edit=sysop:move=sysop]

OCA: New page: '''Unreleased structure''' The entry 8g01 is ON HOLD Authors: Description: Category: Unreleased Structures