OCA at 10:01, 20 December 2023

OCA — Wed, 20 Dec 2023 10:01:04 GMT

←Older revision		Revision as of 10:01, 20 December 2023
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-	'''Unreleased structure'''

-	~~The entry~~ 8g4y is ~~ON HOLD until Paper~~ Publication	+	==Structure of ZNRF3 ECD bound to peptide MK1-3.6.10==
		+	<StructureSection load='8g4y' size='340' side='right'caption='[[8g4y]], [[Resolution\|resolution]] 1.41Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[8g4y]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8G4Y OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8G4Y FirstGlance]. <br>
		+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.41Å</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8g4y FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8g4y OCA], [https://pdbe.org/8g4y PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8g4y RCSB], [https://www.ebi.ac.uk/pdbsum/8g4y PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8g4y ProSAT]</span></td></tr>
		+	</table>
		+	== Disease ==
		+	[https://www.uniprot.org/uniprot/ZNRF3_HUMAN ZNRF3_HUMAN] Adrenocortical carcinoma.
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/ZNRF3_HUMAN ZNRF3_HUMAN] E3 ubiquitin-protein ligase that acts as a negative regulator of the Wnt signaling pathway by mediating the ubiquitination and subsequent degradation of Wnt receptor complex components Frizzled and LRP6. Acts on both canonical and non-canonical Wnt signaling pathway. Acts as a tumor suppressor in the intestinal stem cell zone by inhibiting the Wnt signaling pathway, thereby restricting the size of the intestinal stem cell zone (PubMed:22575959). Along with RSPO2 and RNF43, constitutes a master switch that governs limb specification (By similarity).[UniProtKB:Q08D68]<ref>PMID:22575959</ref>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Selective and precise activation of signaling transduction cascades is key for cellular reprogramming and tissue regeneration. However, the development of small- or large-molecule agonists for many signaling pathways has remained elusive and is rate limiting to realize the full clinical potential of regenerative medicine. Focusing on the Wnt pathway, here we describe a series of disulfide-constrained peptides (DCPs) that promote Wnt signaling activity by modulating the cell surface levels of ZNRF3, an E3 ubiquitin ligase that controls the abundance of the Wnt receptor complex FZD/LRP at the plasma membrane. Mechanistically, monomeric DCPs induce ZNRF3 ubiquitination, leading to its cell surface clearance, ultimately resulting in FZD stabilization. Furthermore, we engineered multimeric DCPs that induce expansive growth of human intestinal organoids, revealing a dependence between valency and ZNRF3 clearance. Our work highlights a strategy for the development of potent, biologically active Wnt signaling pathway agonists via targeting of ZNRF3.

-	~~Authors~~:	+	Potent and selective binders of the E3 ubiquitin ligase ZNRF3 stimulate Wnt signaling and intestinal organoid growth.,Kschonsak YT, Gao X, Miller SE, Hwang S, Marei H, Wu P, Li Y, Ruiz K, Dorighi K, Holokai L, Perampalam P, Tsai WK, Kee YS, Agard NJ, Harris SF, Hannoush RN, de Sousa E Melo F Cell Chem Biol. 2023 Nov 30:S2451-9456(23)00421-X. doi: , 10.1016/j.chembiol.2023.11.006. PMID:38056465<ref>PMID:38056465</ref>

-	~~Description~~:	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased~~ Structures]]	+	</div>
		+	<div class="pdbe-citations 8g4y" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Homo sapiens]]
		+	[[Category: Large Structures]]
		+	[[Category: Synthetic construct]]
		+	[[Category: Harris SF]]
		+	[[Category: Wu P]]

OCA at 05:38, 22 March 2023

OCA — Wed, 22 Mar 2023 05:38:23 GMT

←Older revision		Revision as of 05:38, 22 March 2023
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 8g4y is ON HOLD	+	The entry 8g4y is ON HOLD until Paper Publication

	Authors:		Authors:

OCA: Protected "8g4y" [edit=sysop:move=sysop]

OCA — Wed, 22 Feb 2023 10:19:15 GMT

Protected "8g4y" [edit=sysop:move=sysop]

←Older revision

Revision as of 10:19, 22 February 2023

OCA: New page: '''Unreleased structure''' The entry 8g4y is ON HOLD Authors: Description: Category: Unreleased Structures

OCA — Wed, 22 Feb 2023 10:19:12 GMT

New page: '''Unreleased structure''' The entry 8g4y is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 8g4y is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]

8g4y - Revision history

OCA at 10:01, 20 December 2023

OCA at 05:38, 22 March 2023

OCA: Protected "8g4y" [edit=sysop:move=sysop]

OCA: New page: '''Unreleased structure''' The entry 8g4y is ON HOLD Authors: Description: Category: Unreleased Structures