8gr6 - Revision history

OCA at 21:26, 28 June 2023

OCA — Wed, 28 Jun 2023 21:26:49 GMT

←Older revision		Revision as of 21:26, 28 June 2023
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	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[8gr6]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptococcus_sanguinis_SK36 Streptococcus sanguinis SK36]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8GR6 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8GR6 FirstGlance]. <br>		<table><tr><td colspan='2'>[[8gr6]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptococcus_sanguinis_SK36 Streptococcus sanguinis SK36]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8GR6 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8GR6 FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=MHS:N1-METHYLATED+HISTIDINE'>MHS</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.06Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=MHS:N1-METHYLATED+HISTIDINE'>MHS</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8gr6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8gr6 OCA], [https://pdbe.org/8gr6 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8gr6 RCSB], [https://www.ebi.ac.uk/pdbsum/8gr6 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8gr6 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8gr6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8gr6 OCA], [https://pdbe.org/8gr6 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8gr6 RCSB], [https://www.ebi.ac.uk/pdbsum/8gr6 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8gr6 ProSAT]</span></td></tr>
	</table>		</table>

OCA at 09:39, 21 June 2023

OCA — Wed, 21 Jun 2023 09:39:57 GMT

←Older revision		Revision as of 09:39, 21 June 2023
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-	'''Unreleased structure'''

-	~~The entry~~ 8gr6 is ~~ON HOLD until Paper~~ Publication	+	==Crystal Structure of pilus-specific Sortase C from Streptococcus sanguinis==
		+	<StructureSection load='8gr6' size='340' side='right'caption='[[8gr6]], [[Resolution\|resolution]] 2.06Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[8gr6]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptococcus_sanguinis_SK36 Streptococcus sanguinis SK36]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8GR6 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8GR6 FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=MHS:N1-METHYLATED+HISTIDINE'>MHS</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8gr6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8gr6 OCA], [https://pdbe.org/8gr6 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8gr6 RCSB], [https://www.ebi.ac.uk/pdbsum/8gr6 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8gr6 ProSAT]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/A3CPB4_STRSV A3CPB4_STRSV]
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Dental plaque is a complex microbial biofilm community of many species and a major cause of oral infections and infectious endocarditis. Plaque development begins when primary colonizers attach to oral tissues and undergo coaggregation. Primary colonizers facilitate cellular attachment and inter-bacterial interactions through sortase-dependent pili (or fimbriae) extending out from their cell surface. Consequently, the sortase enzyme is viewed as a potential drug target for controlling biofilm formation and avoiding infection. Streptococcus sanguinis is a primary colonizing bacterium whose pili consist of three different pilin subunits that are assembled together by the pilus-specific (C-type) SsaSrtC sortase. Here, we report on the crystal structure determination of the recombinant wild-type and active-site mutant forms of SsaSrtC. Interestingly, the SsaSrtC structure exhibits an open-lid conformation, although a conserved DPX motif is lacking in the lid. Based on molecular docking and structural analysis, we identified the substrate-binding residues essential for pilin recognition and pilus assembly. We also demonstrated that while recombinant SsaSrtC is enzymatically active toward the five-residue LPNTG sorting motif peptide of the pilins, this activity is significantly reduced by the presence of zinc. We further showed that rutin and alpha-crocin are potential candidate inhibitors of the SsaSrtC sortase via structure-based virtual screening and inhibition assays. The structural knowledge gained from our study will provide the means to develop new approaches that target pilus-mediated attachment, thereby preventing oral biofilm growth and infection.

-	~~Authors~~:	+	Crystal structure of the pilus-specific sortase from early colonizing oral Streptococcus sanguinis captures an active open-lid conformation.,Yadav S, Parijat P, Krishnan V Int J Biol Macromol. 2023 Jul 15;243:125183. doi: 10.1016/j.ijbiomac.2023.125183. , Epub 2023 Jun 3. PMID:37276901<ref>PMID:37276901</ref>

-	~~Description~~:	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased~~ Structures]]	+	</div>
		+	<div class="pdbe-citations 8gr6" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Streptococcus sanguinis SK36]]
		+	[[Category: Krishnan V]]
		+	[[Category: Parijat P]]
		+	[[Category: Yadav S]]

OCA at 04:08, 26 October 2022

OCA — Wed, 26 Oct 2022 04:08:16 GMT

←Older revision		Revision as of 04:08, 26 October 2022
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 8gr6 is ON HOLD	+	The entry 8gr6 is ON HOLD until Paper Publication

	Authors:		Authors:

OCA: Protected "8gr6" [edit=sysop:move=sysop]

OCA — Wed, 07 Sep 2022 15:42:03 GMT

Protected "8gr6" [edit=sysop:move=sysop]

←Older revision

Revision as of 15:42, 7 September 2022

OCA: New page: '''Unreleased structure''' The entry 8gr6 is ON HOLD Authors: Description: Category: Unreleased Structures

OCA — Wed, 07 Sep 2022 15:42:02 GMT

New page: '''Unreleased structure''' The entry 8gr6 is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 8gr6 is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]